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Figure 4.
Figure 4. NLVS inhibitor. (a) F[o] -F[c] electron density
map contoured at 2.6s, computed using calculated phases from
model without inhibitor, showing that inhibitor is present in
upper hexamer only. The two pairs of crystallographically
independent HslV subunits of the HslU(DI)[6]HslV[12] complex
that is aligned along the 3-fold axis are shown; subunits from
HslU(DI)-complexed HslV are red and yellow; from uncomplexed
HslV, green and cyan. Thr1, with which the inhibitor reacts,
shown in magenta in all subunits. (b) Ribbon drawing of
superposition of HslU(DI)-complexed and uncomplexed HslV, with
NLVS inhibitor. Where HslV protomers are similar in
conformation, as originally defined by Sousa et al.,[15.]
structure is green; where they differ, HslU(DI)-complexed HslV
is blue; uncomplexed HslV is yellow. Inhibitor model is red. (c)
Stereo view of superposition of complexed and uncomplexed HslV
near inhibitor. Selected amino acid side-chains and segments of
polypeptide backbone that hydrogen-bond the inhibitor are shown.
For a detailed description of interactions with inhibitor, see
Sousa et al.[23.] C^a backbone trace of HslU(DI)-complexed
subunits, cyan; backbone trace of uncomplexed subunits, yellow;
carbon atoms of inhibitor, magenta; carbon atoms of amino acid
side-chains, green; oxygen atoms, red; nitrogen atoms, blue;
sulfur atoms, yellow; iodine atoms, purple.
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