Figure 4 - full size

Figure 4.
Fig. 4. Differential scanning calorimetry data for the unfolding of wild-type arginase (solid line), R308A (dashed and dotted line), R308E (dotted line), and R308K (dashed line) shown as excess molar heat capacity versus temperature. All scans are normalized per mole of arginase subunit. Conditions were as follows: 20 mM NaPO[4], 150 mM NaCl, 0.1 mM MnCl[2], pH 7.4, and 0.4 mg/ml arginase.