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Figure 3.
Mutational analysis of Pak1 binding sequence. Analytical SEC
of WT-LC8 (A) and individual point mutants (B-K) in
SMT3-Pak1-(204-226) was used to qualitatively assess the role of
each residue in the LC8-binding region of Pak1. SDS-PAGE of
fractions eluting between 10.1 and 10.5 ml is shown for each
experiment. The expected position of LC8 through SDS-PAGE is
indicated with an asterisk. Each experiment was performed three
times.
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