Figure 3. Fig. 3. Calorimetric titration of 12.5 μM Eps8L1 SH3 with 0.25 mM CD3ε peptide (PPVPNPDYEPIR) in 10 mM Tris–HCl buffer (pH 7.5), 50 mM NaCl, and 25 μM DTT at 25 °C.