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Figure 3.
Fig. 3. The overall structure of the GRIP1 PDZ12 tandem in
complex with the Fras1 peptide. (a) Ribbon diagram
representation showing the stereoview of the backbone structure
of the GRIP1 PDZ12 tandem in complex with the Fras1 peptide.
PDZ1 (residues 48–135), PDZ2 (residues 145–240), and
Loop[1–2] (residues 136–144) are colored blue, green, and
yellow, respectively. (b) A semitransparent surface
representation of the PDZ12 tandem showing that the two PDZ
domains interact with each other in a front-to-back fashion to
form a structurally intact supramodule. The figure also
illustrates that the hypothetical target-binding groove of PDZ2
(highlighted with a red oval) is occupied by residues from the
βA-, αA-, and the αA/βD-loop regions of PDZ1 and, therefore,
inaccessible to peptide ligands. (c) Overlay plot of the
backbone structures of PDZ1 and PDZ2 showing the similarity of
their overall conformation. The different conformation of the
βB/βC-loop between the two PDZ domains is highlighted by a
purple oval.
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