Figure 3 - full size

Figure 3.
FIG. 3. The electron density maps of the substrates and cofactor. A, the omit F[O]-F[C] electron density map of the UDP molecule and Mn2+ ion, contoured at 1.6 (gray) and 6.0 (blue), respectively, superimposed with a ball-and-stick model colored according to atom types (nitrogen, blue; carbon, black; oxygen, red; phosphorous, purple; manganese, orange). B, the omit F[O]-F[C] electron density map of the N-acetyllactosamine, contoured at 1.6 (gray), superimposed with a ball-and-stick model. C, the interactions between Mn2+, UDP, and Asp197 side chain of GlcAT-P. The Mn2+ interactions are shown in blue dashed lines.