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Figure 3.
Figure 3. Amino acid sequence alignment of PYRIN domains,
PYRIN domain structure and distribution of charged residues. A,
Amino acid sequence alignment of 35 PYRIN domains from human and
mouse and two from zebrafish. The PYRIN domain of ASC is shown
at the top, together with its sequence numbering. The amino acid
sequence of the FADD DED domain is shown at the bottom for
comparison. The location of the helices and buried side-chains
with less than 5% solvent exposure is indicated for the ASC
PYRIN domain and the FADD DED domain by bars and filled squares,
respectively. Arrows identify positions of significant
differences between the PYRIN domains and the DED consensus. The
consensus sequence of PYRIN domains is indicated below the PYRIN
sequences, where upper case letters indicate conservation of
distinct amino acids and lower case letters indicate
conservation of (h)ydrophobic, (c)harged, (p)olar, a(l)iphatic,
and (t)iny amino acid side-chains. Consensus characters were
assigned when >80% of the residues of a column belong to the
same amino acid class. hs, Homo sapiens; mm, Mus musculus;
PYRIN, pyrin protein; z, zebrafish; ASC, apoptosis-associated
speck-like protein containing a CARD; POP1, pyrin-only protein
1; CIAS1, cold autoinflammatory syndrome 1 (also called PYPAF1
or cryopyrin); CRYOPYR_11, cryopyrin-like protein 1; NALP2,
NACHT-, LRR-, and PYD-containing protein 2 (also called PYPAF2);
NLP2_l1, NALP2-like protein 1; NLP3_l1, NALP3-like protein 1;
PYPAF1-7: PYRIN-containing APAF1-like protein 1-7; PYPAF4_l1-3,
PYPAF4 like proteins 1, 2 and 3; DEFCAP, death effector
filament-forming Ced-4-like apoptosis protein; IFI16, interferon
gamma-inducible protein 16; IFI16_l1-2, IFI16-like proteins 1
and 2; IFI203 and IFI204, interferon-activatable proteins 203
and 204; MNDA, myeloid cell nuclear differentiation antigen;
AIM2, absent in melanoma 2; MATER,
maternal-antigen-that-embryos-require; ANGIN2_l1-3, angiogenin
inhibitor 2-like proteins 1-3; MCMI1, mast cell
maturation-inducible protein-like protein; DKFZ, predicted
protein from DKFZ institute transcript; ENSMUSP00000038294 and
ENSMUSP00000048095: hypothetical proteins predicted by the
ENSEMBL genome annotation project. hs_POP1 is also called
ASC2.[30. and 46.]
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