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Figure 3.
Fig. 3. Stereo figure showing conformational changes cased
by ATP binding. (A) Overlay of subdomains 1 and 2 of Arp3 from
the apo-(red) and ATP (blue) complexes. (B) Overlay of Arp3
based on the superposition of the entire Arp3 subunits from
Apo-containing (red), ADP-containing (blue), and ATP-containing
(cyan) complexes. ATP is shown in yellow. DYNDOM-defined
rotation axes required to bring the apo-Arp3 in alignment with
each of the nucleotide-containing structures are blue for
ADP-Arp3 and green for ATP-Arp3. The dotted black lines indicate
distances measured to compare cleft closure. Lengths in Å
of A-C are as follows: apo-Arp3, 9.84, 8.47, and 16.08;
ADP-Arp3, 8.28, 8.84, and 15.02; ATP-Arp3, 8.35, 6.75, and
13.10; actin (PDB entry 1ATN [PDB]
), 8.35, 5.49, and 10.36. The coordinate error estimate by
Luzzati plot is 0.36 Å for the ADP complex and 0.38
Å for the ATP complex (44).
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