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Figure 3.
FIG. 3. Overview of the ternary
Oct1·Sox2·Hoxb1-DNA complex. A, ribbon diagram of
the ternary complex (Sox2, green; POU[S], red; POU[HD], gold;
and Hoxb1-DNA, blue). The side chain of Met-13 that intercalates
between base pairs 6 and 7 located at the center of the
Sox2-induced DNA bend is also shown. A dashed line represents
the disordered 32-residue linker that connects POU[S] and
POU[HD]. (This region is disordered in solution in both the
binary and ternary complexes, as well as in the binary crystal
structures, Refs. 7 and 26). B, diagrammatic representation of
POU[S]/Sox2 interface in the ternary complex formed by two
anti-parallel helices (Sox2, green and residues denoted in
italic type; POU[S], red). Solid and dashed lines indicate van
der Waals contacts and electrostatic interactions, respectively.
C, detailed view of the POU[S]/Sox2 interface. The side chain
atoms are color coded according to atom type (oxygen, red;
nitrogen, blue; carbon, cyan; sulfur, yellow). The backbone of
Sox2, POU[S], and Hoxb1-DNA are depicted as tubes in green, red,
and blue, respectively.
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