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Figure 3.
Figure 3. Cyro-EM Reconstruction of Rat Liver Cytosol p97
at 18 Å(A) Side view of p97, perpendicular to the 6-fold
molecular axis, shows a truncated barrel-like structure with
side holes. The main body of the molecule shows pseudo 2-fold
symmetry along an axis perpendicular to the 6-fold, although
there are protruding densities at one end (top). The overall
molecular dimensions are indicated. The 145 Å diameter of
the EM map is less than the 160 Å observed in the N D1
crystal structure, which we attribute to the ill-defined N
domain in the EM reconstruction.(B) Cut-open view of (A) reveals
a cage-like structure formed from the two-ring layers
corresponding to the N D1 (blue) and D2 hexamers (green).(C) Top
view, down the 6-fold axis, corresponding to the N D1 hexamer
(blue), reveals a large annulus, surface pockets, and
disconnected densities, and is different in appearance to the D2
layer. The black arrow indicates the pocket that coincides with
the nucleotide binding pocket indicated by the arrow in Figure
1C.(D) Bottom view, corresponding to the D2 hexamer (green),
shows a closed annulus and several distinctive surface features.
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