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Figure 2.
Co-structure of the PUL domain with the C-terminal p97
peptide. A, electrostatic surface representation was generated
with a gradient from −10 (red) to 10 (blue) kT/e of the PUL
domain only. The bound p97 peptide is show in green stick
format. B, surface representation of the PUL domain is shown in
light gray with conserved residues in purple. C, stereoscopic
view of the terminal p97 peptide residues LYG^806 bound to the
PUL domain shown with helices in schematic format and labeled.
PUL residues within proximity are shown in stick format and
labeled. The hydrogen bond is shown as a black dashed line;
water molecules are shown as red cross-hairs. D, schematic
representation of p97 (black) interactions with PLAA (blue). E,
three-dimensional alignment of the PLAA (green) and PNGase
(cyan)-bound p97 peptides.
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