Figure 2.
FIGURE 2. Structural details of intermolecular contacts
with ribbon representation of the protein (blue) and peptide
(pink) backbone. A, the top view of the binding surface displays
the interactions between the side chains of Phe-3 and Tyr-10
from the peptide and helix I and II of mCA-CTD. B, the side view
of the complex displays the interactions that anchor Leu-6 and
Tyr-9 from the peptide using Leu-211 and Met-215 from helix IV.
C, the top view of the x-ray structure of CAI in complex with
CA-CTD (2BUO). D, superposition of the backbone C atoms of
CA-CTD (pink) and mCA-CTD (green) based on alignment generated
from residues in helix I, helix III, and helix IV (r.m.s.d. =
0.8 Å). When helix II is included, the r.m.s.d. increases
to 1.3 Å. Residues that are important for binding the
target peptide and rearranged through the helix movement are
indicated in the figure. The PDB code for CA-CTD structure used
in the alignment is 1A8O. The figures were generated in MOLMOL
2.1 (30).
atoms of
CA-CTD (pink) and mCA-CTD (green) based on alignment generated
from residues in helix I, helix III, and helix IV (r.m.s.d. =
0.8 Å). When helix II is included, the r.m.s.d. increases
to 1.3 Å. Residues that are important for binding the
target peptide and rearranged through the helix movement are
indicated in the figure. The PDB code for CA-CTD structure used
in the alignment is 1A8O. The figures were generated in MOLMOL
2.1 (30).