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Figure 2.
Figure 2. Biochemical characterization of wild-type (WT) Ubc9
and mutants. (a) Initial rates of reaction versus p53
substrate concentration, fit to rectangular hyperbolas
(Methods), are shown for wild-type Ubc9, Ubc9-N85Q, Ubc9-Y87A
and Ubc9-D127A. Left and right y-axes indicate rates for
wild-type and mutant enzymes, respectively. (b) Bar charts
depicting the kinetic constants apparent K[d] and k[2] for
wild-type and mutant Ubc9, plotted on a log scale (see
Supplementary Table 1). Error bars in a and b are  s.d.
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