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Figure 2.
Figure 2. (a) The 2F[o] -F[c] omit map contoured at 1s for
the trithymine oligonucleotide. (b) Protein-DNA interactions.
The trinucleotide binds at the entrance to a channel in the
interface between the b-hairpin loop and residues comprising
domains 1a and 1b. The majority of contacts involve hydrogen
bonds between the phosphate groups of the thymine nucleotides
(5'-T1 to T3-3') and residues Thr481 in domain 3, Lys67 in
domain 1a, Ser 91 at the N terminus of the b-hairpin loop and
Ser141 (also in domain 1a). The furanose sugar and base of T3
stack against the aryl moieties of Tyr146 and Tyr96,
respectively.
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