|
Figure 2.
Figure 2. The conserved pocket of DIAP1-BIR1 is involved in
DrICE interaction and inhibition. (a) Identification of
mutations on BIR1 that result in loss of DrICE inhibition.
Various BIR1 proteins were examined for their ability to inhibit
DrICE activity. The double mutants K77D K79D and D94K E99K did
not inhibit DrICE activity. (b) Inhibition of DrICE correlates
with its interaction with DIAP1-BIR1. In this gel filtration
assay, various BIR1 proteins were individually incubated with
the active DrICE protein. The mixture was applied to a gel
filtration analysis from which contiguous fractions were
visualized on SDS-PAGE by Coomassie blue staining. (c) The
structure of DIAP1-BIR2 (ref. 32) showing the predicted surface
location of the mutated residues in BIR1. The residues whose
corresponding mutation in BIR1 resulted in loss of interaction
with DrICE are red. The corresponding BIR1 residues are
indicated in parentheses. The conserved surface pocket among
most BIR domains is indicated by a purple oval circle. This
panel was generated using MolScript41.
|
