|
Figure 2.
Figure 2. Crystal structures of the ROR  LBD
in complex with ATRA and ALRT. (a) Stereo view of the
backbone superposition of the structures of the ROR  LBD
(yellow) in complex with stearate (orange) and the SRC-1 peptide
(red) (PDB entry 1K4W) and of the ROR LBD
(green) in complex with ATRA (dark blue) and the SRC-1 peptide.
(b) Electron density omit maps of the bound ligands. The maps
were calculated at 2.1 Å for ATRA (left) and 1.5 Å for ALRT
(right). Contour levels are 2.0  and
2.5 ,
respectively; labels refer to the canonical helix numbering. (c)
Anchoring of the carboxylate of ATRA in two different LBDs.
Left, ATRA (yellow) and stearate (orange) in ROR ;
the superposition was made on the protein atoms. Right, ATRA in
hRAR  (PDB
entry 2LBD). In ROR ,
ATRA forms two water mediated hydrogen bonds to Arg306 and
Arg309 whereas in RAR it forms one direct hydrogen bond to
Arg278. (d) Stereo view of the superposition of stearate
(orange), ATRA (yellow) and ALRT (green) in the ROR ligand-binding
pocket. The protein atoms follow the standard color code.
|
