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Figure 1.
Figure 1. Solution NMR Structures of Eaf3 in the Free State
and Linked to Histone H3K[C]36me2
(A) Left: Stereo view of
the 20 lowest-energy structures of Eaf3 chromo barrel domain
(1–113 aa) showing only backbone N, C^α, and C′ after
superposition of residues 8–41 and 55–111. The rmsd is 0.54
and 1.16 Å for the backbone atoms N, C^α, and C′ and
for all heavy atoms of residues 8–41 and 55–111,
respectively. Right: Ribbon representation of the lowest-energy
structure of Eaf3 chromo barrel domain. The helices (α) and β
strands (β) are colored green and blue, respectively. N and C
termini are also indicated.
(B) Left: Stereo view of the 20
lowest-energy structures of Eaf3-H3K[C]36me2 (1–134 aa)
showing only backbone N, C^α, and C′ after superposition of
residues 8–41, 55–111, and 127–130. The Eaf3 chromo barrel
domain (1–115 aa) is colored black, while the linker
(116–119 aa) and H3K[C]36me2 (120–134 aa) are shaded blue
and orange, respectively. The rmsd is 0.61 and 1.13 Å for
the backbone atoms N, C^α, and C′ and for all heavy atoms of
residues 8–41, 55–111, and 127–130, respectively. Right:
Cartoon representation of the lowest-energy structure of
Eaf3-H3K[C]36me2; ribbon for Eaf3 and stick for a portion of
H3K[C]36me2. The helices (α) and β strands (β) are indicated.
Color coding is the same as in (A).
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