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Figure 1.
Figure 1. Structure of SOS^DH-PH-cat(A) Domain organization
of SOS and overview of a ternary Ras:SOS complex. The crystal
structure of the Ras:SOS^cat:Ras^Y64A•GppNp ternary complex is
shown (Margarit et al., 2003; PDB code 1NVV). The helical
hairpin of the Cdc25 domain is shown in orange.(B) The
crystal structure of SOS^DH-PH-cat. Two orthogonal views are
shown with coloring according to the diagram shown in (A).(C)
Comparison of SOS^DH-PH-cat with the structure of the ternary
Ras:SOS^cat:Ras•GTP complex (PDB code 1NVV). The structures
were aligned through superpositioning of the two respective Rem
domains of SOS^DH-PH-cat and SOS^cat. Ras at the catalytic site
is not shown for clarity (see [A]). Note that the distal
Ras^Y64A•GppNp (green) in the ternary complex overlaps with
the DH domain of SOS^DH-PH-cat. A close-up view of the Rem-Cdc25
interface is shown (right).
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