Figure 1.
FIG. 1. Structures of the PDE-IBMX complexes. A, ribbon
diagram of PDE4D2-IBMX. The -helices are colored as
cyan, and blue color represents 3[10] helices. The first metal
ion is interpreted as zinc, as discussed previously (31, 33),
whereas the second metal ion (Me2) is ambiguous. B, ribbon
diagram of PDE5A1-IBMX. The second metal ion was assigned as
magnesium because 0.2 M MgSO[4] was used in the crystallization
buffer. C, the structural superposition between PDE4D2 and
PDE5A1. The cyan ribbons represent the conserved core structures
between PDE4D2 and PDE5A1. The variable regions are drawn in
gold for PDE4D2 and green for PDE5A1. D, the correspondence of
amino acid sequence to the secondary structures.
-helices are colored as
cyan, and blue color represents 3[10] helices. The first metal
ion is interpreted as zinc, as discussed previously (31, 33),
whereas the second metal ion (Me2) is ambiguous. B, ribbon
diagram of PDE5A1-IBMX. The second metal ion was assigned as
magnesium because 0.2 M MgSO[4] was used in the crystallization
buffer. C, the structural superposition between PDE4D2 and
PDE5A1. The cyan ribbons represent the conserved core structures
between PDE4D2 and PDE5A1. The variable regions are drawn in
gold for PDE4D2 and green for PDE5A1. D, the correspondence of
amino acid sequence to the secondary structures.