|
Figure 1.
Figure 1. Domain architecture of (a) c-Myc and (b) Bin1.
Conserved regions of c-Myc are indicated as NTD (N-terminal
domain), CTD (C-terminal domain), MB1 (Myc box 1), MB2 (Myc box
2), BR (basic region), HLH (helix-loop-helix), and LZ (leucine
zipper). The Thr58 and Ser62 phosphorylation sites are indicated
by P. The different Bin1 domains are indicated as BAR
(Bin1/amphiphysin/RVS167), U1 (unknown-1), U2 (unknown-2), MBD
(c-Myc binding domain), and SH3 (Src homology 3). The position
of the original murine clone #99 is shown relative to the human
Bin1 sequence (black bar). The tumor-specific exon 12A splicing
event is depicted against the Bin1+13 primary structure. The
SH3-binding motifs of c-Myc and Bin1 are underlined. The
construct with the entire MBD domain deleted is designated as
Bin1ΔMBD. The C-terminal Bin1 constructs used in this work are
represented schematically as Bin1C−12A, which includes a
discontinuous amino acid sequence (residues 270–303 and
347–482), encoding the MBD and SH3 domains, but lacking the
12A sequence (304–346), Bin1C+12A that includes, in addition
to the MBD and SH3, the tumor-specific exon 12A sequence
(270–482), Bin1CΔPxxP in which the PxxP in exon 12A has been
deleted, Bin1C(SH3) that encodes only the SH3 domain (402–482)
and Bin1 MBD that encodes the MBD (346–402) domain alone.
Residue numbering used in this work is based on the
Bin1(−10+12A) isoform (Genbank accession AAC23750.1).
|
