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Figure 1.
Figure 1. Location of the I546V mutation and its effects on
platelet adhesion. a, Stereo view of the vWF A1 domain
presented as a ribbon structure, with  -strands
in yellow and  -helices
in purple connected by loops. Note the position of the side
chain of Ile 546. The loops preceding and following strand 3,
site of the major conformational changes caused by the I546V
substitution, are colored in green. The figure was produced with
Bobscript^28. b, Velocity of platelets rolling on wild-type or
I546V mutant vWF A1 domain in a flow field with wall shear rate
of 1500 s^ -1. Results are presented as number of platelets
within defined velocity categories. Platelets rolling on the
mutant A1 domain had a median velocity of 4.2  m
s^-1 compared to 44.7 m
s^ -1 on the wild type control. A video clip showing the real
time interaction of flowing platelets with wild type and mutant
A1 domain is provided as supplementary material.
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