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Figure 1.
Figure 1 Wild-type Kar3 and mutated residues. (A) The conserved
switch I (SwI, green) and switch II (SwII, cyan) residues are
indicated in Kar3+N11 together with helices  3
and 4
(gray). The switch II loop between R632 and the end of helix
4
(dotted line) is disordered and is not present in the model.
Residues mutated in the Kar3 N650K uncoupling mutant (gray),
Kar3 SwII R632A mutant (gray) and Kar3 salt-bridge mutants
(R598A, green; E631A, cyan) are shown space-filled and enlarged
in (B). (B) N650 of the Kar3 N650K uncoupling mutant interacts
with R632 of SwII in wild-type Kar3. The salt bridge forms
between R598 of SwI and E631 of SwII in wild-type Kar3. ADP
(black) and Mg2+ (red) are shown as ball-and-stick models.
Figure produced using RIBBONS (Carson, 1997).
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