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Figure 1.
Figure 1. Structure of Rho13N Bound to RNA(a) Experimental
electron density map superposed on a ball-and-stick model of the
refined RNA. The map was generated using phases from the
3-fold-averaged poly-serine molecular replacement solution and
the observed structure factors. Gold contours are at 1.0 σ and
cyan at 2.5 σ.(b) Refined 2F[o] − F[c], model-phased map of
the same region. Gold contours are at 1.3 σ.(c) Front view of
the secondary structure of Rho13N monomer (green) shown with
bound oligoribocytidine (ball-and-stick) in the OB-fold cleft.
The cleft is formed on the surface of strands β2 and β3, with
the β1–β2 and β4–β5 loops forming parts of the lower and
upper walls, respectively. Helices and strands are labeled.(d)
View as in (c), rotated 90° about the vertical axis ([a] and
[b] generated by BOBSCRIPT and RASTER3D [[40 and 22]]; (c) and
(d) generated by RIBBONS [ [12]]).
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