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Figure 1.
Figure 1. The Protein and DNA Constructs Used in
Crystallization and Their Contacts(a and b) The upstream (a) and
downstream (b) positioned DNA-binding regions of human RevErbα
are numbered starting with the first conserved cysteine. The
authentic numbers appear in the parentheses. Dashed lines
indicate amino- and carboxy-terminal residues not found in the
electron density maps. Closed and open arrows indicate direct
and water-mediated hydrogen bonds to the DNA bases,
respectively. Closed and open boxes indicate direct and
water-mediated hydrogen bonds to the DNA phosphates. Colored
circles indicate Van der Waals contacts with the DNA, and closed
black circles indicate residues that mediate subunit
dimerization. A symbol indicates one or more such contacts.(c)
The 20 base pair DNA is numbered from the 5′ end, with arrows
indicating the half-site repeats and boxes indicating the spacer
and 5′ flanking sequence. Shown are the upstream (green) and
downstream (red) contacts from the RevErb subunits.
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