_EC 6.3.4.3 Formate--tetrahydrofolate ligase. 22 PDB entries  
EC 6.-.-.- Ligases. [1,425 PDB entries]
EC 6.3.-.- Forming carbon-nitrogen bonds. [732 PDB entries]
EC 6.3.4.- Other carbon--nitrogen ligases. [226 PDB entries]
EC 6.3.4.3 Formate--tetrahydrofolate ligase. [22 PDB entries]    
1a4i

Pathway: Folate Coenzymes
Reaction: Atp + formate + tetrahydrofolate = adp + phosphate + 10-formyltetrahydrofolate.
 


ATP
+
formate
+
tetrahydrofolate
=
ADP
+
phosphate
+
10-formyltetrahydrofolate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Formyltetrahydrofolate synthetase. Tetrahydrofolate formylase. Tetrahydrofolic formylase.
Comments: In eukaryotes occurs as a trifunctional enzyme also having Ec 1.5.1.5 and Ec 3.5.4.9 activity.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 22 PDB entries in enzyme class E.C.6.3.4.3

  PDB code Protein
1a4i
Human tetrahydrofolate dehydrogenase / cyclohydrolase
Source: Homo sapiens. Human. Organism_taxid: 9606. Cellular_location: cytoplasm. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (285 residues) CATH domains: 3.40.50.720 3.40.50.10860
Bound ligand:   Het Group NDP is 56.00% similar to enzyme product ATP
1dia
Human methylenetetrahydrofolate dehydrogenase / cyclohydrolase complexed with NADP and inhibitor ly249543
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (285 residues) CATH domains: 3.40.50.720 3.40.50.10860
Bound ligand:   Het Group NAP is 56.00% similar to enzyme product ATP
1dib
Human methylenetetrahydrofolate dehydrogenase / cyclohydrolase complexed with NADP and inhibitor ly345899
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (285 residues) CATH domains: 3.40.50.720 3.40.50.10860
Bound ligands:   Het Group NAP is 56.00% similar to enzyme product ATP
  Het Group L34 corresponds to enzyme product
1dig
Human methylenetetrahydrofolate dehydrogenase / cyclohydrolase complexed with NADP and inhibitor ly374571
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (285 residues) CATH domains: 3.40.50.720 3.40.50.10860
Bound ligands:   Het Group ACT is 75.00% similar to enzyme reactant ATP
  Het Group NAP is 56.00% similar to enzyme product
  Het Group L37 is 47.00% similar to enzyme product
1eg7
The crystal structure of formyltetrahydrofolate synthetase f moorella thermoacetica
Source: Moorella thermoacetica. Organism_taxid: 1525. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (549 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
1fp7
Monovalent cation binding sites in n10-formyltetrahydrofolat synthetase from moorella thermoacetica
Source: Moorella thermoacetica. Organism_taxid: 1525. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (549 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
1fpm
Monovalent cation binding sites in n10-formyltetrahydrofolat synthetase from moorella thermoacetica
Source: Moorella thermoacetica. Organism_taxid: 1525. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (549 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
2eo2
Solution structure of the insertion region (510-573) of fthfs domain from mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like protein
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: mthfd1l. Other_details: cell free protein synthesis
Chain: A (71 residues) CATH domain: 0.0.0.0
3do6
Crystal structure of putative formyltetrahydrofolate synthetase (tm1766) from thermotoga maritima at 1.85 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: fhs, tm_1766. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (543 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligand:   Het Group EDO is 40.00% similar to enzyme reactant ATP
3pzx
Native crystal structure of n10-formyltetrahydrofolate synth
Source: Moorella thermoacetica. Clostridium thermoaceticum. Organism_taxid: 1525. Gene: fhs. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (550 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
3qb6
Crystal strucutre of n10-formyltetrahydrofolate synthetase w
Source: Moorella thermoacetica. Clostridium thermoaceticum. Organism_taxid: 1525. Gene: fhs, moorella. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (551 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligand:   Het Group FOL corresponds to enzyme reactant ATP
3qus
Crystal strucutre of n10-formyltetrahydrofolate synthetase w
Source: Moorella thermoacetica. Clostridium thermoaceticum. Organism_taxid: 1525. Gene: fhs, moorella. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (550 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligand:   Het Group AGS is 93.75% similar to enzyme reactant ATP
3rbo
Crystal structure of n10-formyltetrahydrofolate synthetase w and formylphosphate
Source: Moorella thermoacetica. Organism_taxid: 1525. Gene: fhs, moorella. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (551 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligands:   Het Group ADP corresponds to enzyme product ATP
  Het Group XPO is 71.43% similar to enzyme product
3sin
Crystal strucutre of n10-formyltetrahydrofolate synthetase w and formylphosphate
Source: Moorella thermoacetica. Organism_taxid: 1525. Gene: fhs, moorella. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (551 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligand:   Het Group XPO is 71.43% similar to enzyme product ATP
4ioj
N10-formyltetrahydrofolate synthetase from moorella thermoac sulfate
Source: Moorella thermoacetica. Organism_taxid: 264732. Strain: atcc 39073. Gene: fhs, moth_0109. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (557 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
4iok
N10-formyltetrahydrofolate synthetase from moorella thermoac adp, xpo
Source: Moorella thermoacetica. Organism_taxid: 264732. Strain: atcc 39073. Gene: fhs, moth_0109. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (557 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligands:   Het Group ADP corresponds to enzyme product ATP
  Het Group XPO is 71.43% similar to enzyme product
4iol
N10-formyltetrahydrofolate synthetase from moorella thermoac adp/zd9 and xpo
Source: Moorella thermoacetica. Organism_taxid: 264732. Strain: atcc 39073. Gene: fhs, moth_0109. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (557 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligands:   Het Group ADP corresponds to enzyme product ATP
  Het Group XPO is 71.43% similar to enzyme product
4iom
N10-formyltetrahydrofolate synthetase from moorella thermoac folate
Source: Moorella thermoacetica. Organism_taxid: 264732. Strain: atcc 39073. Gene: fhs, moth_0109. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (557 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligand:   Het Group FOL corresponds to enzyme reactant ATP
4jim
Native crystal strucutre of n10-formyltetrahydrofolate synth
Source: Moorella thermoacetica. Organism_taxid: 264732. Strain: atcc 39073. Gene: fhs, moorella, moth_0109. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (557 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
4jjk
Crystal strucutre of n10-formyltetrahydrofolate synthetase w
Source: Moorella thermoacetica. Organism_taxid: 264732. Strain: atcc 39073. Gene: fhs, moorella, moth_0109. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (557 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligands:   Het Group EDO is 40.00% similar to enzyme reactant ATP
  Het Group FOL corresponds to enzyme reactant
4jjz
Crystal strucutre of n10-formyltetrahydrofolate synthetase w and formylphosphate
Source: Moorella thermoacetica. Organism_taxid: 264732. Strain: atcc 39073. Gene: fhs, moorella, moth_0109. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (554 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligands:   Het Group ADP corresponds to enzyme product ATP
  Het Group XPO is 71.43% similar to enzyme product
4jki
Crystal strucutre of n10-formyltetrahydrofolate synthetase w zd9331, formylphosphate, and adp
Source: Moorella thermoacetica. Organism_taxid: 264732. Strain: atcc 39073. Gene: fhs, moorella, moth_0109. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (557 residues) CATH domains: 3.40.50.300 3.30.1510.10 3.10.410.10
Bound ligands:   Het Group ADP corresponds to enzyme product ATP
  Het Group XPO is 71.43% similar to enzyme product