_EC 6.3.2.34 Coenzyme F420-1:gamma-L-glutamate ligase. 2 PDB entries  
EC 6.-.-.- Ligases. [1,580 PDB entries]
EC 6.3.-.- Forming carbon-nitrogen bonds. [793 PDB entries]
EC 6.3.2.- Acid--amino-acid ligases (peptide synthases). [329 PDB entries]
EC 6.3.2.34 Coenzyme F420-1:gamma-L-glutamate ligase. [2 PDB entries]    
2g9i

Reaction: Gtp + coenzyme F420-1 + L-glutamate = gdp + phosphate + coenzyme gamma- F420-2.
 


GTP
+
coenzyme F420-1
+
L-glutamate
=
GDP
+
phosphate
+ coenzyme gamma- F420-2
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Comments: This protein catalyzes the successive addition of two glutamate residues to cofactor F420 by two distinct and independent reactions. In the first reaction (EC 6.3.2.31) the enzyme attaches a glutamate via its alpha-amine group to F420-0. In the second reaction, which is described here, the enzyme catalyzes the addition of a second L-glutamate residue to the gamma-carboxyl of the first glutamate.
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There are 2 PDB entries in enzyme class E.C.6.3.2.34

  PDB code Protein
2g9i
Crystal structure of homolog of f420-0:gamma-glutamyl ligase archaeoglobus fulgidus reveals a novel fold.
Source: Archaeoglobus fulgidus. Organism_taxid: 224325. Strain: dsm 4304. Gene: cofe. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (233 residues) CATH domains: 3.30.1330.100 3.90.1660.10
2phn
Crystal structure of an amide bond forming f420-gamma glutam from archaeoglobus fulgidus
Source: Archaeoglobus fulgidus dsm 4304. Organism_taxid: 224325. Strain: dsm 4304, vc-16, jcm 9628, nbrc 100126. Atcc: 49558. Gene: cofe, af_2256. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (248 residues) CATH domains: 3.30.1330.100 3.90.1660.10