_EC 6.3.2.17 Tetrahydrofolate synthase. 11 PDB entries  
EC 6.-.-.- Ligases. [1,591 PDB entries]
EC 6.3.-.- Forming carbon-nitrogen bonds. [803 PDB entries]
EC 6.3.2.- Acid--amino-acid ligases (peptide synthases). [339 PDB entries]
EC 6.3.2.17 Tetrahydrofolate synthase. [11 PDB entries]    
1fgs

Pathway: Folate Biosynthesis (late stages)
Reaction: Atp + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = adp + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).
 


ATP
+ tetrahydropteroyl-(gamma-Glu)(n)
+
L-glutamate
=
ADP
+
phosphate
+ tetrahydropteroyl-(gamma-Glu)(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Folate polyglutamate synthetase. Folylpoly(gamma-glutamate) synthase. Folylpoly-gamma-glutamate synthase. Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Folylpolyglutamate synthase. Folylpolyglutamate synthetase. Folylpolyglutamyl synthetase. Formyltetrahydropteroyldiglutamate synthetase. Fpgs. N(10)-formyltetrahydropteroyldiglutamate synthetase.
Comments: In some bacteria, a single protein catalyzes both this activity and that of Ec 6.3.2.12, the combined activity of which leads to the formation of the coenzyme polyglutamated tetrahydropteroate (H(4)PteGlu(n)), i.e. various tetrahydrofolates (H(4)folate). In contrast, the activities are located on separate proteins in most eukaryotes studied to date. In Arabidopsis thaliana, this enzyme is present as distinct isoforms in the mitochondria, the cytosol and the chloroplast. Each isoform is encoded by a separate gene, a situation that is unique among eukaryotes.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 11 PDB entries in enzyme class E.C.6.3.2.17

  PDB code Protein
1fgs
Folylpolyglutamate synthetase from lactobacillus casei
Source: Lactobacillus casei. Organism_taxid: 1582. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (393 residues) CATH domains: 3.40.1190.10 3.90.190.20
Bound ligand:   Het Group POP is 55.00% similar to enzyme product phosphate
1jbv
Fpgs-amppcp complex
Source: Lactobacillus casei. Organism_taxid: 1582. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (404 residues) CATH domains: 3.40.1190.10 3.90.190.20
Bound ligand:   Het Group ACP is 81.00% similar to enzyme product ADP
1jbw
Fpgs-amppcp-folate complex
Source: Lactobacillus casei. Organism_taxid: 1582. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (410 residues) CATH domains: 3.40.1190.10 3.90.190.20
Bound ligand:   Het Group ACQ is 72.00% similar to enzyme product ADP
1w78
E.Coli folc in complex with dhpp and adp
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (414 residues) CATH domains: 3.40.1190.10 3.90.190.20
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
1w7k
E.Coli folc in complex with adp, without folate substrate
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (411 residues) CATH domains: 3.40.1190.10 3.90.190.20
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
2gc5
G51s mutant of l. Casei fpgs
Source: Lactobacillus casei. Organism_taxid: 1582. Gene: fgs. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (408 residues) CATH domains: 3.40.1190.10 3.90.190.20
2gc6
S73a mutant of l. Casei fpgs
Source: Lactobacillus casei. Organism_taxid: 1582. Gene: fgs. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (407 residues) CATH domains: 3.40.1190.10 3.90.190.20
2gca
Apo form of l. Casei fpgs
Source: Lactobacillus casei. Organism_taxid: 1582. Gene: fgs. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (410 residues) CATH domains: 3.40.1190.10 3.90.190.20
2gcb
G51s/s52t double mutant of l. Casei fpgs
Source: Lactobacillus casei. Organism_taxid: 1582. Gene: fgs. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (407 residues) CATH domains: 3.40.1190.10 3.90.190.20
2vor
Crystal structures of mycobacterium tuberculosis folylpolyglutamate synthase complexed with adp and amppcp
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (442 residues) CATH domains: 3.40.1190.10 3.90.190.20
Bound ligand:   Het Group ACP is 81.25% similar to enzyme product ADP
2vos
Mycobacterium tuberculosis folylpolyglutamate synthase complexed with adp
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (448 residues) CATH domains: 3.40.1190.10 3.90.190.20
Bound ligands:   Het Group GOL is 45.45% similar to enzyme reactant L-glutamate
  Het Group ADP corresponds to enzyme product ADP