_EC 6.3.2.10 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase. 6 PDB entries  
EC 6.-.-.- Ligases. [1,591 PDB entries]
EC 6.3.-.- Forming carbon-nitrogen bonds. [803 PDB entries]
EC 6.3.2.- Acid--amino-acid ligases (peptide synthases). [339 PDB entries]
EC 6.3.2.10 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase. [6 PDB entries]    
1gg4

Pathway: Peptidoglycan Biosynthesis (Part 1)
Reaction: Atp + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl- D-alanine = Adp + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D- glutamyl-L-lysyl-D-alanyl-D-alanine.
 


ATP
+
UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
+
D-alanyl- D-alanine
=
ADP
+
phosphate
+ UDP-N-acetylmuramoyl-L-alanyl-gamma-D- glutamyl-L-lysyl-D-alanyl-D-alanine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): MurF synthetase. UDP-MurNAc-L-Ala-D-Glu-L-Lys:D-Ala-D-Ala ligase. UDP-MurNAc-pentapeptide synthetase. UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine synthetase. UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D- alanyl-D-alanine synthetase. UDP-N-acetylmuramoylalanine-D-glutamyl-lysine--D-alanyl-D-alanine ligase. UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D- alanine ligase.
Comments: Involved with Ec 6.3.2.4, Ec 6.3.2.7 or Ec 6.3.2.13, Ec 6.3.2.8 and Ec 6.3.2.9 in the synthesis of a cell-wall peptide. Also catalyzes the reaction when the C-terminal residue of the tripeptide is meso-2,4-diaminoheptanedioate (acylated at its L-center), linking the D-Ala--D-Ala to the carboxy group of the L-center. Formerly Ec 6.3.2.15.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 6 PDB entries in enzyme class E.C.6.3.2.10

  PDB code Protein
1gg4
Crystal structure of escherichia coli udpmurnac-tripeptide d-alanyl-d-alanine-adding enzyme (murf) at 2.3 angstrom resolution
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (439 residues) CATH domains: 3.90.190.20 3.40.1190.10 3.40.1390.10
2am1
Sp protein ligand 1
Source: Streptococcus pneumoniae. Organism_taxid: 171101. Strain: r6. Gene: murf. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (454 residues) CATH domains: 3.40.1390.10 3.40.1190.10 3.90.190.20
2am2
Sp protein ligand 2
Source: Streptococcus pneumoniae. Organism_taxid: 171101. Strain: r6. Gene: murf. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (454 residues) CATH domains: 3.40.1390.10 3.40.1190.10 3.90.190.20
3zl8
Crystal structure of murf ligase from thermotoga maritima in complex with adp
Source: Thermotoga maritima. Organism_taxid: 2336. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (427 residues) CATH domains: Unassigned 3.40.1190.10 3.90.190.20
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
3zm5
Crystal structure of murf ligase in complex with cyanothiophene inhibitor
Source: Streptococcus pneumoniae r6. Organism_taxid: 171101. Expressed in: escherichia coli. Expression_system_taxid: 668369.
Chain: A (454 residues) CATH domains: 3.40.1390.10 3.40.1190.10 3.90.190.20
3zm6
Crystal structure of murf ligase in complex with cyanothiophene inhibitor
Source: Streptococcus pneumoniae. Organism_taxid: 171101. Strain: r6. Expressed in: escherichia coli. Expression_system_taxid: 668369.
Chain: A (454 residues) CATH domains: 3.40.1390.10 3.40.1190.10 3.90.190.20