_EC 6.3.1.8 Glutathionylspermidine synthase. 9 PDB entries  
EC 6.-.-.- Ligases. [1,580 PDB entries]
EC 6.3.-.- Forming carbon-nitrogen bonds. [793 PDB entries]
EC 6.3.1.- Acid--ammonia (or amide) ligases (amide synthases). [70 PDB entries]
EC 6.3.1.8 Glutathionylspermidine synthase. [9 PDB entries]    
2io7

Reaction: Glutathione + spermidine + Atp = glutathionylspermidine + adp + phosphate.
 


Glutathione
+
spermidine
+
ATP
=
glutathionylspermidine
+
ADP
+
phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Gamma-L-glutamyl-L-cysteinyl-glycine:spermidine ligase (ADP-forming). Glutathione:spermidine ligase (ADP-forming). Glutathionylspermidine synthetase. Gsp synthetase.
Cofactor(s): Mg(2+).
Comments: Involved in the synthesis of trypanothione in trypanosomatids. The enzyme from Escherichia coli is bifunctional and also catalyzes the Ec 3.5.1.78 reaction, resulting in a net hydrolysis of Atp.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 9 PDB entries in enzyme class E.C.6.3.1.8

  PDB code Protein
2io7
E. Coli bifunctional glutathionylspermidine synthetase/amidase incomplex with mg2+ and amppnp
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (587 residues) CATH domains: Unassigned Unassigned
Bound ligand:   Het Group ANP is 81.00% similar to enzyme product ADP
2io8
E. Coli bifunctional glutathionylspermidine synthetase/amidase incomplex with mg2+ and adp
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (598 residues) CATH domains: Unassigned Unassigned
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
2io9
E. Coli bifunctional glutathionylspermidine synthetase/amidase incomplex with mg2+ ,gsh and adp
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (601 residues) CATH domains: Unassigned Unassigned
Bound ligands:   Het Group GSH is 95.00% similar to enzyme reactant glutathione
  Het Group ADP corresponds to enzyme product ADP
2ioa
E. Coli bifunctional glutathionylspermidine synthetase/amidase incomplex with mg2+ and adp and phosphinate inhibitor
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (589 residues) CATH domains: Unassigned Unassigned
Bound ligands:   Het Group GGA is 43.00% similar to enzyme reactant glutathione
  Het Group ADP corresponds to enzyme product ADP
2iob
E. Coli bifunctional glutathionylspermidine synthetase/amidase apo protein
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (571 residues) CATH domains: Unassigned Unassigned
3a2y
E. Coli gsp amidase c59a complexed with gsp
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: gsp, b2988, jw2956. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (185 residues)
Bound ligand:   Het Group TS5 corresponds to enzyme product N(1)-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine
3a2z
E. Coli gsp amidase cys59 sulfenic acid
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: gsp, b2988, jw2956. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (190 residues)
3a30
E. Coli gsp amidase c59 acetate modification
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: gsp, b2988, jw2956. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (179 residues)
3o98
Glutathionylspermidine synthetase/amidase c59a complex with gsp
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (599 residues) CATH domains: Unassigned Unassigned
Bound ligands:   Het Group TS5 corresponds to enzyme product N(1)-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine
  Het Group ADP corresponds to enzyme product ADP