_EC 4.6.1.13 Phosphatidylinositol diacylglycerol-lyase. 27 PDB entries  
EC 4.-.-.- Lyases. [3,636 PDB entries]
EC 4.6.-.- Phosphorus-oxygen lyases. [193 PDB entries]
EC 4.6.1.- Phosphorus-oxygen lyases. [193 PDB entries]
EC 4.6.1.13 Phosphatidylinositol diacylglycerol-lyase. [27 PDB entries]    
1aod

Pathway: 1-Phosphatidyl-myo-inositol Metabolism
Reaction: 1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.
 


1-phosphatidyl-1D-myo-inositol
=
1D-myo-inositol 1,2-cyclic phosphate
+
1,2-diacyl-sn-glycerol
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): 1-phosphatidylinositol phosphodiesterase. Monophosphatidylinositol phosphodiesterase. Phosphatidylinositol phospholipase C.
Comments: This enzyme is bacterial. Activity is also found in animals, but this activity is due to the presence of Ec 3.1.4.11. Formerly Ec 3.1.4.10.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 27 PDB entries in enzyme class E.C.4.6.1.13

  PDB code Protein
1aod
Phosphatidylinositol-specific phospholipasE C from listeria monocytogenes
Source: Listeria monocytogenes. Organism_taxid: 1639. Strain: egd serotype 1/2a. Cell_line: bl21. Cellular_location: secreted into medium. Gene: plca. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (274 residues) CATH domain: 3.20.20.190
Bound ligand:   Het Group INS is 80.00% similar to enzyme product 1D-myo-inositol 1,2-cyclic phosphate
1gym
Phosphatidylinositol-specific phospholipasE C in complex with glucosamine-(alpha-1-6)-myo-inositol
Source: Bacillus cereus. Organism_taxid: 1396. Gene: pi-plc gene. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (296 residues) CATH domain: 3.20.20.190
Bound ligand:   Het Group MYG is 46.00% similar to enzyme product 1D-myo-inositol 1,2-cyclic phosphate
1ptd
Phosphatidylinositol-specific phospholipasE C
Source: Bacillus cereus. Organism_taxid: 1396. Gene: pi-plc gene. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (296 residues) CATH domain: 3.20.20.190
1ptg
Phosphatidylinositol-specific phospholipasE C in complex with myo-inositol
Source: Bacillus cereus. Organism_taxid: 1396. Gene: pi-plc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (296 residues) CATH domain: 3.20.20.190
Bound ligand:   Het Group INS is 80.00% similar to enzyme product 1D-myo-inositol 1,2-cyclic phosphate
1t6m
X-ray structure of the r70d pi-plc enzyme: insight into the role of calcium and surrounding amino acids on active site geometry and catalysis.
Source: Bacillus thuringiensis. Organism_taxid: 1428. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (296 residues) CATH domain: 3.20.20.190
2or2
Structure of the w47a/w242a mutant of bacterial phosphatidylinositol-specific phospholipasE C
Source: Bacillus thuringiensis. Organism_taxid: 1428. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (296 residues) CATH domain: 3.20.20.190
2plc
Phosphatidylinositol-specific phospholipasE C from listeria monocytogenes
Source: Listeria monocytogenes. Organism_taxid: 1639. Strain: egd serotype 1-2a. Cell_line: bl21. Cellular_location: secreted into medium. Gene: plca. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (274 residues) CATH domain: 3.20.20.190
2ptd
Phosphatidylinositol-specific phospholipasE C mutant d198e
Source: Bacillus cereus. Organism_taxid: 1396. Cellular_location: secreted into medium. Gene: pi-plc gene. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: gene cloned in escherichia coli
Chain: A (296 residues) CATH domain: 3.20.20.190
3ea1
Crystal structure of the y247s/y251s mutant of phosphatidylinositol-specific phospholipasE C from bacillus thuringiensis
Source: Bacillus thuringiensis. Organism_taxid: 1428. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (296 residues) CATH domain: 3.20.20.190
3ea2
Crystal structure of the myo-inositol bound y247s/y251s mutant of phosphatidylinositol-specific phospholipasE C from bacillus thuringiensis
Source: Bacillus thuringiensis. Organism_taxid: 1428. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (296 residues) CATH domain: 3.20.20.190
Bound ligand:   Het Group INS is 80.00% similar to enzyme product 1D-myo-inositol 1,2-cyclic phosphate
3ea3
Crystal structure of the y246s/y247s/y248s/y251s mutant of phosphatidylinositol-specific phospholipasE C from bacillus thuringiensis
Source: Bacillus thuringiensis. Organism_taxid: 1428. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (296 residues) CATH domain: 3.20.20.190
3ptd
Phosphatidylinositol-specific phospholipasE C mutant d274s
Source: Bacillus cereus. Organism_taxid: 1396. Cellular_location: secreted into medium. Gene: pi-plc gene. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: gene cloned in escherichia coli
Chain: A (296 residues) CATH domain: 3.20.20.190
3v16
An intramolecular pi-cation latch in phosphatidylinositol-sp phospholipasE C from s.Aureus controls substrate access to site
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman
Chain: A (303 residues) CATH domain: 3.20.20.190
Bound ligand:   Het Group INS is 80.00% similar to enzyme product 1D-myo-inositol
3v18
Structure of the phosphatidylinositol-specific phospholipase staphylococcus aureus
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman
Chain: A (304 residues) CATH domain: 3.20.20.190
3v1h
Structure of the h258y mutant of phosphatidylinositol-specif phospholipasE C from staphylococcus aureus
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman
Chain: A (306 residues) CATH domain: 3.20.20.190
Bound ligand:   Het Group INS is 80.00% similar to enzyme product 1D-myo-inositol
4f2b
Modulation of s.Aureus phosphatidylinositol-specific phospho membrane binding
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman. Gene: plc, nwmn_0041. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (304 residues) CATH domain: 3.20.20.190
4f2t
Modulation of s.Aureus phosphatidylinositol-specific phospho membrane binding.
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman. Gene: plc, nwmn_0041. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (302 residues) CATH domain: 3.20.20.190
4f2u
Structure of the n254y/h258y double mutant of the phosphatidylinositol-specific phospholipasE C from s.Aureus
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman. Gene: plc, nwmn_0041. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (304 residues) CATH domain: 3.20.20.190
4i8y
Structure of the unliganded n254y/h258y mutant of the phosphatidylinositol-specific phospholipasE C from s. Aureu
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman. Gene: plc, nwmn_0041. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (303 residues) CATH domain: 3.20.20.190
4i90
Structure of the n254y/h258y mutant of the phosphatidylinosi specific phospholipasE C from s. Aureus bound to choline
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman. Gene: plc, nwmn_0041. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (302 residues) CATH domain: 3.20.20.190
4i9j
Structure of the n254y/h258y mutant of the phosphatidylinosi specific phospholipasE C from s. Aureus bound to dic4pc
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman. Gene: plc, nwmn_0041. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (302 residues) CATH domain: 3.20.20.190
Bound ligand:   Het Group XP5 is 43.24% similar to enzyme reactant 1-phosphatidyl-1D-myo-inositol
4i9m
Structure of the n254y/h258y mutant of the phosphatidylinosi specific phospholipasE C from staphylococcus aureus bound t
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman. Gene: plc, nwmn_0041. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (304 residues) CATH domain: 3.20.20.190
4i9t
Structure of the h258y mutant of the phosphatidylinositol-sp phospholipasE C from staphylococcus aureus
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 426430. Strain: newman. Gene: plc, nwmn_0041. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (305 residues) CATH domain: 3.20.20.190
Bound ligand:   Het Group INS is 80.00% similar to enzyme product 1D-myo-inositol
4ptd
Phosphatidylinositol-specific phospholipasE C mutant d274n
Source: Bacillus cereus. Organism_taxid: 1396. Cellular_location: secreted into medium. Gene: pi-plc gene. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: gene cloned in escherichia coli
Chain: A (296 residues) CATH domain: 3.20.20.190
5ptd
Phosphatidylinositol-specific phospholipasE C mutant h32a
Source: Bacillus cereus. Organism_taxid: 1396. Cellular_location: secreted into medium. Gene: pi-plc gene. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: gene cloned in escherichia coli
Chain: A (296 residues) CATH domain: 3.20.20.190
6ptd
Phosphatidylinositol-specific phospholipasE C mutant h32l
Source: Bacillus cereus. Organism_taxid: 1396. Cellular_location: secreted into medium. Gene: pi-plc gene. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: gene cloned in escherichia coli
Chain: A (296 residues) CATH domain: 3.20.20.190
7ptd
Phosphatidylinositol-specific phospholipasE C mutant r163k
Source: Bacillus cereus. Organism_taxid: 1396. Cellular_location: secreted into medium. Gene: pi-plc gene. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: gene cloned in escherichia coli
Chain: A (296 residues) CATH domain: 3.20.20.190