_EC 4.4.1.16 Selenocysteine lyase. 12 PDB entries  
EC 4.-.-.- Lyases. [3,613 PDB entries]
EC 4.4.-.- Carbon-sulfur lyases. [120 PDB entries]
EC 4.4.1.- Carbon-sulfur lyases. [120 PDB entries]
EC 4.4.1.16 Selenocysteine lyase. [12 PDB entries]    
1c0n

Reaction: L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor.
 


L-selenocysteine
+ reduced acceptor
= selenide
+
L-alanine
+ acceptor
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Selenocysteine beta-lyase. Selenocysteine reductase.
Cofactor(s): Pyridoxal 5'-phosphate.
 

Pyridoxal 5'-phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
Comments: Dithiothreitol or 2-mercaptoethanol can act as the reducing agent in the reaction. The enzyme from animals does not act on cysteine, serine or chloroalanine, while the enzyme from bacteria shows activity with cysteine (cf. Ec 2.8.1.7).
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 12 PDB entries in enzyme class E.C.4.4.1.16

  PDB code Protein
1c0n
Csdb protein, nifs homologue
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (404 residues) CATH domains: 3.90.1150.10 3.40.640.10
Bound ligands:   Het Group ACY is 66.67% similar to enzyme product L-alanine
  Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
1i29
Crystal structure of csdb complexed with l-propargylglycine
Source: Escherichia coli. Organism_taxid: 562. Gene: csdb or sufs. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (405 residues) CATH domains: 3.90.1150.10 3.40.640.10
Bound ligands:   Het Group LPG corresponds to enzyme product L-alanine
  Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
1jf9
Crystal structure of selenocysteine lyase
Source: Escherichia coli. Organism_taxid: 562. Gene: nifs/csdb. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (405 residues) CATH domains: 3.90.1150.10 3.40.640.10
Bound ligand:   Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
1kmj
E. Coli nifs/csdb protein at 2.0a with the cysteine persulfi intermediate (residue css).
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (405 residues) CATH domains: 3.90.1150.10 3.40.640.10
Bound ligand:   Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
1kmk
E. Coli nifs/csdb protein at 2.20a with the cysteine persele intermediate (residue csz).
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (405 residues) CATH domains: 3.90.1150.10 3.40.640.10
Bound ligands:   Het Group SEC is 71.43% similar to enzyme product L-alanine
  Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
3a9x
Crystal structure of rat selenocysteine lyase
Source: Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: scly. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (388 residues) CATH domains: 3.90.1150.10 Unassigned 3.40.640.10
Bound ligand:   Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
3a9y
Crystal structure of rat selenocysteine lyase in complex wit cysteine
Source: Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: scly. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (403 residues) CATH domains: 3.90.1150.10 Unassigned 3.40.640.10
Bound ligands:   Het Group CYS is 85.71% similar to enzyme product L-alanine
  Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
3a9z
Crystal structure of ras selenocysteine lyase in complex wit selenopropionate
Source: Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: scly. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (403 residues) CATH domains: 3.90.1150.10 Unassigned 3.40.640.10
Bound ligands:   Het Group SLP is 71.43% similar to enzyme product L-alanine
  Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
3gzc
Structure of human selenocysteine lyase
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: scl, scly. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (403 residues) CATH domains: 3.90.1150.10 Unassigned 3.40.640.10
Bound ligand:   Het Group PLR is 93.75% similar to enzyme cofactor pyridoxal
3gzd
Human selenocysteine lyase, p1 crystal form
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: scl, scly. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (397 residues) CATH domains: 3.90.1150.10 Unassigned 3.40.640.10
Bound ligand:   Het Group PLR is 93.75% similar to enzyme cofactor pyridoxal
4lw2
Structural changes during cysteine desulfurase csda and sulf acceptor csde interactions provide insight into the trans- persulfuration
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b2810, csda, jw2781, ygdj. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (399 residues) CATH domains: 3.90.1150.10 3.40.640.10
4lw4
Structural changes during cysteine desulfurase csda and sulf acceptor csde interactions provide insight into the trans- persulfuration
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b2810, csda, jw2781, ygdj. Expressed in: escherichia coli. Expression_system_taxid: 562. Organism_taxid: 714962. Strain: ihe3034. Gene: csde, ecok1_3187.
Chains: A, B (392 residues) CATH domains: 3.90.1150.10 3.40.640.10