_EC 4.2.3.61 5-epiaristolochene synthase. 12 PDB entries  
EC 4.-.-.- Lyases. [3,638 PDB entries]
EC 4.2.-.- Carbon-oxygen lyases. [1,772 PDB entries]
EC 4.2.3.- Acting on phosphates. [169 PDB entries]
EC 4.2.3.61 5-epiaristolochene synthase. [12 PDB entries]    
1hx9

Reaction: (2E,6E)-farnesyl diphosphate = (+)-5-epiaristolochene + diphosphate.
 


(2E,6E)-farnesyl diphosphate
= (+)-5-epiaristolochene
+
diphosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): 5-epi-aristolochene synthase. Farnesyl pyrophosphate cyclase.
Comments: Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 12 PDB entries in enzyme class E.C.4.2.3.61

  PDB code Protein
1hx9
Crystal structure of teas w273s form 1
Source: Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (523 residues) CATH domains: 1.50.10.130 1.10.600.10
Bound ligand:   Het Group FHP is 66.67% similar to enzyme product aristolochene
1hxa
Crystal structure of teas w273s form 2
Source: Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (528 residues) CATH domains: 1.50.10.130 1.10.600.10
Bound ligand:   Het Group FHP is 66.67% similar to enzyme product aristolochene
1hxc
Crystal structure of teas c440w
Source: Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (516 residues) CATH domains: 1.50.10.130 1.10.600.10
Bound ligand:   Het Group FHP is 66.67% similar to enzyme product aristolochene
1hxg
Crystal structure of teas w273s/c440w
Source: Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (534 residues) CATH domains: 1.50.10.130 1.10.600.10
3lz9
The crystal structure of 5-epi-aristolochene synthase m4 mut complexed with (2-trans,6-trans)-2-fluorofarnesyl diphospha
Source: Nicotiana tabacum. American tobacco,tobacco. Organism_taxid: 4097. Gene: eas3, eas4. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (527 residues) CATH domains: 1.10.600.10 1.50.10.130
Bound ligand:   Het Group FPF is 96.00% similar to enzyme reactant trans,trans-farnesyl diphosphate
3m00
Crystal structure of 5-epi-aristolochene synthase m4 mutant with (2-cis,6-trans)-2-fluorofarnesyl diphosphate
Source: Nicotiana tabacum. American tobacco,tobacco. Organism_taxid: 4097. Gene: eas3, eas4. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (530 residues) CATH domains: 1.10.600.10 1.50.10.130
Bound ligand:   Het Group 2CF is 96.00% similar to enzyme reactant trans,trans-farnesyl diphosphate
3m01
The crystal structure of 5-epi-aristolochene synthase comple (2-trans,6-trans)-2-fluorofarnesyl diphosphate
Source: Nicotiana tabacum. American tobacco,tobacco. Organism_taxid: 4097. Gene: eas3, eas4. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (530 residues) CATH domains: 1.10.600.10 1.50.10.130
Bound ligand:   Het Group FPF is 96.00% similar to enzyme reactant trans,trans-farnesyl diphosphate
3m02
The crystal structure of 5-epi-aristolochene synthase comple (2-cis,6-trans)-2-fluorofarnesyl diphosphate
Source: Nicotiana tabacum. American tobacco,tobacco. Organism_taxid: 4097. Gene: eas3, eas4. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (535 residues) CATH domains: 1.10.600.10 1.50.10.130
Bound ligand:   Het Group 2CF is 96.00% similar to enzyme reactant trans,trans-farnesyl diphosphate
4di5
Co-crystal structure of wt 5-epi-aristolochene synthase from tobaccum with geraniline
Source: Nicotiana tabacum. American tobacco,tobacco. Organism_taxid: 4097. Gene: 5-eas, eas3, eas4. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (535 residues) CATH domains: 1.10.600.10 1.50.10.130
Bound ligands:   Het Group 1GA is 51.85% similar to enzyme reactant (2E,6E)-
  Het Group DPO corresponds to enzyme product diphosphate
5eas
5-epi-aristolochene synthase from nicotiana tabacum
Source: Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Strain: nk326. Cell_line: bl21. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (514 residues) CATH domains: 1.50.10.130 1.10.600.10
5eat
5-epi-aristolochene synthase from nicotiana tabacum with substrate analog farnesyl hydroxyphosphonate
Source: Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Strain: nk326. Cell_line: bl21. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (532 residues) CATH domains: 1.50.10.130 1.10.600.10
Bound ligand:   Het Group FHP is 66.67% similar to enzyme product aristolochene
5eau
5-epi-aristolochene synthase from nicotiana tabacum
Source: Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Strain: bl21 (de3). Cell_line: bl21. Cellular_location: cytoplasm. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (523 residues) CATH domains: 1.50.10.130 1.10.600.10
Bound ligand:   Het Group FFF is 88.89% similar to enzyme reactant trans,trans-farnesyl