_EC 4.2.3.5 Chorismate synthase. 19 PDB entries  
EC 4.-.-.- Lyases. [3,615 PDB entries]
EC 4.2.-.- Carbon-oxygen lyases. [1,754 PDB entries]
EC 4.2.3.- Acting on phosphates. [169 PDB entries]
EC 4.2.3.5 Chorismate synthase. [19 PDB entries]    
1q1l

Pathway: Shikimate and Chorismate Biosynthesis
Reaction: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.
 


5-O-(1-carboxyvinyl)-3-phosphoshikimate
=
chorismate
+
phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): 5-enolpyruvylshikimate-3-phosphate phospholyase.
Cofactor(s): Fmn.
 

FMN
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
Comments: The reaction goes via a radical mechanism that involves reduced Fmn and its semiquinone (Fmnh.). Shikimate is numbered so that the double-bond is between C-1 and C-2, but some earlier papers numbered the ring in the reverse direction. Formerly Ec 4.6.1.4.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 19 PDB entries in enzyme class E.C.4.2.3.5

  PDB code Protein
1q1l
Crystal structure of chorismate synthase
Source: Aquifex aeolicus. Organism_taxid: 63363. Gene: aroc. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B, C, D (336 residues) CATH domain: 3.60.150.10
1qxo
Crystal structure of chorismate synthase complexed with oxidized fmn and epsp
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Gene: aroc, sp1374 or spr1232. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (388 residues) CATH domain: 3.60.150.10
Bound ligands:   Het Group EPS corresponds to enzyme reactant 5-O- (1-carboxyvinyl)-3-phosphoshikimate
  Het Group FMN corresponds to enzyme cofactor FMN
1r52
Crystal structure of the bifunctional chorismate synthase from saccharomyces cerevisiae
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: ygl148w. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (283 residues) CATH domain: 3.60.150.10
1r53
Crystal structure of the bifunctional chorismate synthase fr saccharomyces cerevisiae
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: ygl148w. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (287 residues) CATH domain: 3.60.150.10
1sq1
Crystal structure of the chorismate synthase from campylobac jejuni, northeast structural genomics target br19
Source: Campylobacter jejuni. Organism_taxid: 197. Strain: nctc 11168. Gene: aroc or cj1634. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (288 residues) CATH domain: 3.60.150.10
1u3x
Homology model of chorismate synthase from c. Difficile
Source: Clostridium difficile. Bacteria
Chain: A (351 residues)
1um0
Crystal structure of chorismate synthase complexed with fmn
Source: Helicobacter pylori. Organism_taxid: 210. Gene: aroc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (365 residues) CATH domain: 3.60.150.10
Bound ligand:   Het Group FMN corresponds to enzyme cofactor FMN
1umf
Crystal structure of chorismate synthase
Source: Helicobacter pylori. Organism_taxid: 210. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (365 residues) CATH domain: 3.60.150.10
1ztb
Crystal structure of chorismate synthase from mycobacterium tuberculosis
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: aroc, arof. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (388 residues) CATH domain: 3.60.150.10
2g85
Crystal structure of chorismate synthase from mycobacterium tuberculosis at 2.22 angstrons of resolution
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: aroc. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (386 residues) CATH domain: 3.60.150.10
2o11
Mycobacterium tuberculosis chorismate synthase
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Gene: arof. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (383 residues) CATH domain: 3.60.150.10
2o12
Mycobacterium tuberculosis chorismate synthase in complex wi
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Gene: arof. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (386 residues) CATH domain: 3.60.150.10
Bound ligand:   Het Group FMN corresponds to enzyme cofactor FMN
2qhf
Mycobacterium tuberculosis chorismate synthase in complex wi
Source: Mycobacterium tuberculosis. Strain: h37rv. Gene: aroc, arof. Expressed in: escherichia coli.
Chain: A (392 residues) CATH domain: 3.60.150.10
4bai
Mycobacterium tuberculosis chorismate synthase before exposure to 266 nm uv laser
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (377 residues) CATH domain: 3.60.150.10
4baj
Mycobacterium tuberculosis chorismate synthase after exposure to 266nm uv laser
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (375 residues) CATH domain: 3.60.150.10
4ecd
2.5 angstrom resolution crystal structure of bifidobacterium chorismate synthase
Source: Bifidobacterium longum subsp. Infantis organism_taxid: 391904. Strain: atcc 15697 / dsm 20088 / jcm 1222 / nctc 11817 / s1 gene: aroc, blij_1653, blon_1598. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chains: A, B (274 residues) CATH domain: 3.60.150.10
4lj2
Crystal structure of chorismate synthase from acinetobacter at 3.15a resolution
Source: Acinetobacter baumannii. Organism_taxid: 575584. Strain: atcc 19606. Gene: aroc. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (322 residues) CATH domain: 3.60.150.10
4o90
Crystal structure of chorismate synthase from acinetobacter at 2.6a resolution
Source: Acinetobacter baumannii. Organism_taxid: 575584. Strain: atcc 19606. Gene: aroc. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (294 residues) CATH domain: 3.60.150.10
4ob9
Crystal structure of chorismate synthase from acinetobacter at 2.50a resolution
Source: Acinetobacter baumannii. Organism_taxid: 575584. Strain: atcc 19606. Gene: aroc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (313 residues) CATH domain: 3.60.150.10