_EC 4.2.2.2 Pectate lyase. 36 PDB entries  
EC 4.-.-.- Lyases. [3,636 PDB entries]
EC 4.2.-.- Carbon-oxygen lyases. [1,771 PDB entries]
EC 4.2.2.- Acting on polysaccharides. [117 PDB entries]
EC 4.2.2.2 Pectate lyase. [36 PDB entries]    
1air

Pathway: Pectin and Pectate Lyases
Reaction: Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Other name(s): Alpha-1,4-D-endopolygalacturonic acid lyase. Endo-alpha-1,4-polygalacturonic acid lyase. Endogalacturonate transeliminase. Endopectin methyltranseliminase. Pectate transeliminase. Pectic acid lyase. Pectic acid transeliminase. Pectic lyase. Pectin trans-eliminase. Pga lyase.
Comments: Favors pectate, the anion, over pectin, the methyl ester (which is the preferred substrate of Ec 4.2.2.10). Formerly Ec 4.2.99.3.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 36 PDB entries in enzyme class E.C.4.2.2.2

  PDB code Protein
1air
Pectate lyasE C from erwinia chrysanthemi (ec16) to a resolution of 2.2 angstroms with 128 waters
Source: Erwinia chrysanthemi. Organism_taxid: 556. Strain: ec16. Cellular_location: extracellular. Plasmid: hb101
Chain: A (352 residues) CATH domain: 2.160.20.10
1bn8
Bacillus subtilis pectate lyase
Source: Bacillus subtilis. Organism_taxid: 1423. Strain: s1103
Chain: A (399 residues) CATH domain: 2.160.20.10
1jrg
Crystal structure of the r3 form of pectate lyase a, erwinia chrysanthemi
Source: Erwinia chrysanthemi. Organism_taxid: 556. Strain: ec16. Gene: pela. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (354 residues) CATH domain: 2.160.20.10
1jta
Crystal structure of pectate lyase a (c2 form)
Source: Erwinia chrysanthemi. Organism_taxid: 556. Strain: ec16. Gene: pela. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (361 residues) CATH domain: 2.160.20.10
1o88
Pectate lyasE C from erwinia chrysanthemi at ph 11.2 with 30mm ca2+
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8d
Pectate lyasE C from erwinia chrysanthemi at ph 11.2 with 5mm ca2+
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8e
Pectate lyasE C from erwinia chrysanthemi at ph 11.2 with 1mm ca2+
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8f
Pectate lyasE C from erwinia chrysanthemi at ph 9.5 with 30mm ca2+
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8g
Pectate lyasE C from erwinia chrysanthemi at ph 9.5 with 5mm ca2+
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8h
Pectate lyasE C from erwinia chrysanthemi at ph 9.5 with 0.3mm ca2+ added
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8i
Pectate lyasE C from erwinia chrysanthemi at ph 9.5 with no ca2+ added
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8j
Pectate lyasE C from erwinia chrysanthemi at ph 4.5 with 30mm ca2+
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8k
Pectate lyasE C from erwinia chrysanthemi at ph 4.5 with 20mm ca2+
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8l
Pectate lyasE C from erwinia chrysanthemi at ph 4.5 with 5mm ca2+
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1o8m
Pectate lyasE C from erwinia chrysanthemi at ph 4.5 with no ca2+ added
Source: Erwinia chrysanthemi. Organism_taxid: 556. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (353 residues) CATH domain: 2.160.20.10
1ooc
Mutations in the t1.5 loop of pectate lyase a
Source: Erwinia chrysanthemi. Organism_taxid: 556. Gene: pela. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (361 residues) CATH domain: 2.160.20.10
1pcl
Unusual structural features in the parallel beta-helix in pectate lyases
Source: Erwinia chrysanthemi. Organism_taxid: 556. Gene: ppel748 of pelc
Chain: A (355 residues)
1pe9
Mutations in the t1.5 loop of pectate lyase a
Source: Erwinia chrysanthemi. Organism_taxid: 556. Gene: pela. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (361 residues) CATH domain: 2.160.20.10
1plu
Pectate lyasE C from erwinia chrysanthemi with 1 lu+3 ion in the putative calcium binding site
Source: Erwinia chrysanthemi. Organism_taxid: 556. Strain: ec16. Cellular_location: extracellular. Plasmid: hb101
Chain: A (353 residues) CATH domain: 2.160.20.10
1pxz
1.7 angstrom crystal structure of jun a 1, the major allergen from cedar pollen
Source: Juniperus ashei. Ozark white cedar. Organism_taxid: 13101. Other_details: pollen
Chains: A, B (346 residues) CATH domain: 2.160.20.10
1ru4
Crystal structure of pectate lyase pel9a
Source: Erwinia chrysanthemi. Organism_taxid: 556. Gene: pell. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (400 residues) CATH domain: 2.160.20.10
2bsp
Bacillus subtilis pectate lyase r279k mutant
Source: Bacillus subtilis. Organism_taxid: 1423. Strain: s1103. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (399 residues) CATH domain: 2.160.20.10
2ewe
Crystal structure of pectate lyasE C r218k mutant in complex with pentagalacturonic acid
Source: Erwinia chrysanthemi. Organism_taxid: 556. Strain: ec16. Gene: pelc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (352 residues) CATH domain: 2.160.20.10
2nzm
Hexasaccharide i bound to bacillus subtilis pectate lyase
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: pel, bsu07560. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (399 residues) CATH domain: 2.160.20.10
2o04
Pectate lyase bound to hexasaccharide compound ii
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: pel. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (399 residues) CATH domain: 2.160.20.10
2o0v
Pectate lyase bound to hexasaccharide compound iii
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: pel. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (399 residues) CATH domain: 2.160.20.10
2o0w
Pectate lyase bound to hexasaccharide compound iv
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: pel. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (399 residues) CATH domain: 2.160.20.10
2o17
Pectate lyase bound to hexasaccharide
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: pel. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (399 residues) CATH domain: 2.160.20.10
2o1d
Pectate lyase bound to trisaccharide
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: pel. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (399 residues) CATH domain: 2.160.20.10
2pec
The refined three-dimensional structure of pectate lyasE C from erwinia chrysanthemi at 2.2 angstroms resolution: implications for an enzymatic mechanism
Source: Erwinia chrysanthemi. Organism_taxid: 556. Gene: ppel410 of pelc
Chain: A (352 residues) CATH domain: 2.160.20.10
3b4n
Crystal structure analysis of pectate lyase peli from erwinia chrysanthemi
Source: Erwinia chrysanthemi. Organism_taxid: 556. Gene: peli. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (314 residues) CATH domains: 2.60.40.10 2.160.20.10
3b8y
Crystal structure of pectate lyase peli from erwinia chrysanthemi in complex with tetragalacturonic acid
Source: Erwinia chrysanthemi. Organism_taxid: 556. Gene: peli. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (304 residues) CATH domains: 2.60.40.10 2.160.20.10
3b90
Crystal structure of the catalytic domain of pectate lyase p erwinia chrysanthemi
Source: Erwinia chrysanthemi. Organism_taxid: 556. Gene: peli. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (226 residues) CATH domain: 2.160.20.10
3krg
Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: pel. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (399 residues) CATH domain: 2.160.20.10
3t9g
The crystal structure of family 3 pectate lyase from caldicellulosiruptor bescii
Source: Caldicellulosiruptor bescii. Anaerocellum thermophilum. Organism_taxid: 31899. Gene: athe_1854, cbes_1854. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (196 residues) CATH domain: 2.160.20.10
4ew9
The liganded structure of c. Bescii family 3 pectate lyase
Source: Caldicellulosiruptor bescii. Organism_taxid: 521460. Strain: dsm 6725 / z-1320. Gene: athe_1854. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (195 residues) CATH domain: 2.160.20.10