_EC 4.2.1.59 3-hydroxyacyl-[acyl-carrier-protein] dehydratase. 42 PDB entries  
EC 4.-.-.- Lyases. [3,636 PDB entries]
EC 4.2.-.- Carbon-oxygen lyases. [1,771 PDB entries]
EC 4.2.1.- Hydro-lyases. [1,375 PDB entries]
EC 4.2.1.59 3-hydroxyacyl-[acyl-carrier-protein] dehydratase. [42 PDB entries]    
1mka

Reaction: A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl- carrier protein] + H(2)O.
 


(3R)-3-hydroxyacyl-[acyl-carrier protein]
= trans-2-enoyl-[acyl- carrier protein]
+ H(2)O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): (3R)-3-hydroxyoctanoyl-[acyl-carrier-protein] hydro-lyase. 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase. Beta-hydroxyoctanoyl thioester dehydratase. Beta-hydroxyoctanoyl-ACP-dehydrase. Beta-hydroxyoctanoyl-acyl carrier protein dehydrase. D-3-hydroxyoctanoyl-[acyl carrier protein] dehydratase.
Comments: This enzyme is responsible for the dehydration step of the dissociated (type II) fatty-acid biosynthesis system that occurs in plants and bacteria. The enzyme uses fatty acyl thioesters of Acp in vivo. Different forms of the enzyme may have preferences for substrates with different chain length. For example, the activity of FabZ, the ubiquitous enzyme in bacteria, decreases with increasing chain length. Gram-negative bacteria that produce unsaturated fatty acids, such as Escherichia coli, have another form (FabA) that prefers intermediate
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 42 PDB entries in enzyme class E.C.4.2.1.59

  PDB code Protein
1mka
E. Coli beta-hydroxydecanoyl thiol ester dehydrase modified by its classic mechanism-based inactivator, 3-decynoyl-n- acetyl cysteamine
Source: Escherichia coli. Organism_taxid: 562. Strain: ra244. Gene: faba. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: tac promoter
Chains: A, B (171 residues) CATH domain: 3.10.129.10
Bound ligands:   Het Group DAC is 63.00% similar to enzyme product trans-dec-2-enoyl- [acyl-carrier-protein]
  Het Group DAC is 60.00% similar to enzyme reactant (3R)-3-hydroxydecanoyl-[acyl-carrier-protein]
1mkb
Escherichia coli beta-hydroxydecanoyl thiol ester dehydrase at ph 5 and 21 degrees c
Source: Escherichia coli. Organism_taxid: 562. Strain: ra244. Gene: faba. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: tac promoter
Chains: A, B (171 residues) CATH domain: 3.10.129.10
1u1z
The structure of (3r)-hydroxyacyl-acp dehydratase (fabz)
Source: Pseudomonas aeruginosa. Organism_taxid: 287. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (142 residues) CATH domain: 3.10.129.10
1xkt
Human fatty acid synthase: structure and substrate selectivity of the thioesterase domain
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (260 residues) CATH domains: 3.40.50.1820 1.10.1470.20
2cg5
Structure of aminoadipate-semialdehyde dehydrogenase- phosphopantetheinyl transferase in complex with cytosolic acyl carrier protein and coenzyme a
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_cell_line: de3. Expressed in: escherichia coli
Chain: B (71 residues) CATH domain: 1.10.1200.10
2gll
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase(fabz) from helicobacter pylori
Source: Helicobacter pylori. Organism_taxid: 102617. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
2glm
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase(fabz) from helicobacter pylori complexed with compound 2
Source: Helicobacter pylori. Organism_taxid: 102617. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
2glp
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase(fabz) from helicobacter pylori complexed with compound 1
Source: Helicobacter pylori. Organism_taxid: 102617. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
2glv
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase(fabz) mutant(y100a) from helicobacter pylori
Source: Helicobacter pylori. Organism_taxid: 102617. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H, I, J, K, L (152 residues) CATH domain: 3.10.129.10
2jfd
Structure of the mat domain of human fas
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_cell_line: de3-r3.
Chains: A, B, C, D (404 residues) CATH domains: Unassigned 3.40.366.10 3.30.70.250
2jfk
Structure of the mat domain of human fas with malonyl-coa
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_cell_line: de3-r3.
Chains: A, B, C, D (401 residues) CATH domains: Unassigned 3.40.366.10 3.30.70.250
2pff
Structural insights of yeast fatty acid synthase
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Strain: yit613. Synthetic: yes
Chains: B, E, H (2006 residues) CATH domains: Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned
2png
Type i rat fatty acid synthase acyl carrier protein (acp) domain
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Gene: fasn. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (89 residues) CATH domain: 1.10.1200.10
2px6
Crystal structure of the thioesterase domain of human fatty acid synthase inhibited by orlistat
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: fas. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (253 residues) CATH domains: 3.40.50.1820 1.10.1470.20
Bound ligand:   Het Group DH9 is 48.00% similar to enzyme reactant (3R)-3-hydroxypalmitoyl-[acyl-carrier-protein]
2uv8
Crystal structure of yeast fatty acid synthase with stalled acyl carrier protein at 3.1 angstrom resolution
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Organism_taxid: 4932
Chains: G, H, I (2033 residues) CATH domains: Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned
2vkz
Structure of the cerulenin-inhibited fungal fatty acid synthase type i multienzyme complex
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Organism_taxid: 4932
Chains: G, H, I (2033 residues) CATH domains: Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned
Bound ligand:   Het Group CER is 45.00% similar to enzyme reactant (3R)-3-hydroxypalmitoyl-[acyl-carrier-protein]
3b7j
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase(fabz) from helicobacter pylori complexed with juglone
Source: Helicobacter pylori. Campylobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3cf8
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with quercetin
Source: Helicobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3cf9
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with apigenin
Source: Helicobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3d04
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with sakuranetin
Source: Helicobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3d6x
Crystal structure of campylobacter jejuni fabz
Source: Campylobacter jejuni subsp. Jejuni 81- organism_taxid: 354242. Gene: fabz, cjj81176_0300. Expressed in: escherichia coli.
Chains: A, B, C, D, E, F (137 residues) CATH domain: 3.10.129.10
3doy
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with compound 3i
Source: Helicobacter pylori. Campylobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3doz
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with compound 3k
Source: Helicobacter pylori. Campylobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (151 residues) CATH domain: 3.10.129.10
3dp0
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with compound 3m
Source: Helicobacter pylori. Campylobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3dp1
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with compound 3n
Source: Helicobacter pylori. Campylobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (151 residues) CATH domain: 3.10.129.10
3dp2
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with compound 3j
Source: Helicobacter pylori. Campylobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3dp3
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with compound 3q
Source: Helicobacter pylori. Campylobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3ed0
Crystal structure of (3r)-hydroxyacyl-acyl carrier protein dehydratase (fabz) from helicobacter pylori in complex with emodin
Source: Helicobacter pylori. Organism_taxid: 210. Strain: ss1. Gene: fabz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (152 residues) CATH domain: 3.10.129.10
3hhd
Structure of the human fatty acid synthase ks-mat didomain as a framework for inhibitor design.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: fasn, fas. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108
Chains: A, B, C, D (852 residues) CATH domains: Unassigned Unassigned Unassigned 3.30.70.250
3hmj
Saccharomyces cerevisiae fas type i
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: fas2. Gene: fas1
Chains: G, H, I (2033 residues) CATH domains: Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned Unassigned
Bound ligand:   Het Group CER is 45.00% similar to enzyme reactant (3R)-3-hydroxypalmitoyl-[acyl-carrier-protein]
3q62
Crystal structure of 3-hydroxydecanoyl-(acyl carrier protein dehydratase from yersinia pestis
Source: Yersinia pseudotuberculosis. Organism_taxid: 214092. Strain: co92. Gene: faba, yptb1450. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chains: A, B (170 residues) CATH domain: 3.10.129.10
3tjm
Crystal structure of the human fatty acid synthase thioester with an activate site-specific polyunsaturated fatty acyl a
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: fas, fasn. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (275 residues) CATH domains: 3.40.50.1820 1.10.1470.20
4b0b
Crystal structure of 3-hydroxydecanoyl-acyl carrier protein dehydratase (faba) from pseudomonas aeruginosa in complex with 3-(pyridin-2-yloxy)aniline
Source: Pseudomonas aeruginosa. Organism_taxid: 287. Strain: pa01. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: tuner.
Chains: A, B (170 residues) CATH domain: 3.10.129.10
4b0c
Crystal structure of 3-hydroxydecanoyl-acyl carrier protein dehydratase (faba) from pseudomonas aeruginosa in complex with 3-(pentylthio)-4h-1,2,4-triazole
Source: Pseudomonas aeruginosa. Organism_taxid: 287. Strain: pa01. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: tuner.
Chains: A, B, C, D, E (168 residues) CATH domain: 3.10.129.10
4b0i
Crystal structure of 3-hydroxydecanoyl-acyl carrier protein dehydratase (faba) mutant (h70n) from pseudomonas aeruginosa in complex with 3-hydroxydecanoyl-n-acetyl cysteamine
Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pa01. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: star.
Chains: A, B, C, D, E (168 residues) CATH domain: 3.10.129.10
4b0j
Crystal structure of 3-hydroxydecanoyl-acyl carrier protein dehydratase (faba) from pseudomonas aeruginosa in complex w thienyl)-3-isoxazolyl methanol
Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pa01. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: tuner.
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T (167 residues) CATH domain: 3.10.129.10
4b8u
Crystal structure of 3-hydroxydecanoyl-acyl carrier protein dehydratase (faba) from pseudomonas aeruginosa in complex w n-isobutyl-2-(5-(2-thienyl)-1,2-oxazol-3-yl-)methoxy)acetam
Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: tuner.
Chains: A, B, C, D, E (170 residues) CATH domain: 3.10.129.10
4fq9
Crystal structure of 3-hydroxydecanoyl-acyl carrier protein dehydratase (faba) from pseudomonas aeruginosa
Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pa01. Gene: faba, pa1610. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H, I, J (170 residues) CATH domain: 3.10.129.10
4h4g
Crystal structure of (3r)-hydroxymyristoyl-[acyl-carrier-pro dehydratase from burkholderia thailandensis e264
Source: Burkholderia thailandensis. Organism_taxid: 271848. Gene: fabz, bth_i2038. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H, I (135 residues) CATH domain: 3.10.129.10
4i83
Crystal structure of (3r)-hydroxymyristoyl-acp dehydratase f neisseria meningitidis fam18
Source: Neisseria meningitidis. Organism_taxid: 272831. Strain: fam18. Gene: fabz, nmc0170. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, C, B, D, E, F (144 residues) CATH domain: 3.10.129.10
4keh
Crosslinked crystal structure of type ii fatty synthase dehy faba, and acyl carrier protein, acpp
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: acpp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (166 residues) CATH domain: 3.10.129.10
4piv
Human fatty acid synthase psi/kr tri-domain with NADPH and g
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: fasn, fas. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.
Chains: A, B (610 residues)