_EC 4.2.1.17 Enoyl-CoA hydratase. 41 PDB entries  
EC 4.-.-.- Lyases. [3,636 PDB entries]
EC 4.2.-.- Carbon-oxygen lyases. [1,771 PDB entries]
EC 4.2.1.- Hydro-lyases. [1,375 PDB entries]
EC 4.2.1.17 Enoyl-CoA hydratase. [41 PDB entries]    
1dub

Reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O.
 


(3S)-3-hydroxyacyl-CoA
= trans-2(or 3)-enoyl-CoA
+ H(2)O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Enoyl hydrase. Unsaturated acyl-CoA hydratase.
Comments: Acts in the reverse direction. With cis-compounds, yields (3R)-3-hydroxyacyl-CoA (cf. Ec 4.2.1.74).
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 41 PDB entries in enzyme class E.C.4.2.1.17

  PDB code Protein
1dub
2-enoyl-coa hydratase, data collected at 100 k, ph 6.5
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: liver
Chains: A, B, C, D, E, F (260 residues) CATH domains: 3.90.226.10 1.10.12.10
Bound ligand:   Het Group CAA is 96.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
1ey3
Structure of enoyl-coa hydratase complexed with the substrate dac-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: liver. Organelle: mitochondria. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (258 residues) CATH domains: 3.90.226.10 1.10.12.10
Bound ligand:   Het Group DAK is 82.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
1mj3
Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D, E, F (258 residues) CATH domains: 3.90.226.10 1.10.12.10
Bound ligand:   Het Group HXC is 91.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
1wdk
Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native2)
Source: Pseudomonas fragi. Organism_taxid: 296. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (708 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group ACO is 94.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
1wdl
Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4)
Source: Pseudomonas fragi. Organism_taxid: 296. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (715 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group ACO is 74.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
1wdm
Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3)
Source: Pseudomonas fragi. Organism_taxid: 296. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (707 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group ACO is 94.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
1zcj
Crystal structure of 3-hydroxyacyl-coa dehydrogenase
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (459 residues) CATH domains: Unassigned 3.40.50.720 Unassigned 1.10.1040.10
2c2i
Structure and function of rv0130, a conserved hypothetical protein from m.Tuberculosis
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (149 residues) CATH domain: 3.10.129.10
2d3t
Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form v
Source: Pseudomonas fragi. Organism_taxid: 296. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chains: A, B (708 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group ACO is 94.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
2dub
Enoyl-coa hydratase complexed with octanoyl-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: liver. Cellular_location: mitochondria
Chains: A, B, C, D, E, F (254 residues) CATH domains: 3.90.226.10 1.10.12.10
Bound ligand:   Het Group CO8 is 88.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
2hw5
The crystal structure of human enoyl-coenzyme a (coa) hydrat chain 1, echs1
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: echs1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (260 residues) CATH domains: 3.90.226.10 1.10.12.10
Bound ligand:   Het Group COO is 64.81% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
2ppy
Crystal structure of enoyl-coa hydrates (gk_1992) from geoba kaustophilus hta426
Source: Geobacillus kaustophilus. Organism_taxid: 235909. Strain: hta426. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (264 residues) CATH domains: 3.90.226.10 1.10.12.10
2wtb
Arabidopsis thaliana multifuctional protein, mfp2
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (676 residues) CATH domains: 3.90.226.10 3.40.50.720
2x58
The crystal structure of mfe1 liganded with coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (725 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group COA is 88.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
3gow
Crystal structure of phenylacetic acid degradation protein paag
Source: Thermus thermophilus hb8. Organism_taxid: 300852. Strain: hb8. Gene: ttha0290. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (254 residues) CATH domains: 3.90.226.10 1.10.12.10
3h81
Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: echa8, rv1070c, mt1100, mtv017.23c. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (256 residues) CATH domains: 3.90.226.10 1.10.12.10
3he2
Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Strain: 1719b. Gene: echa6, rv0905, mt0928, mtcy31.33. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (240 residues) CATH domain: 3.90.226.10
3i47
Crystal structure of putative enoyl coa hydratase/isomerase (crotonase) from legionella pneumophila subsp. Pneumophila philadelphia 1
Source: Legionella pneumophila subsp. Pneumoph organism_taxid: 272624. Strain: philadelphia 1. Gene: lpg1828. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (259 residues) CATH domains: 3.90.226.10 1.10.12.10
3moy
Crystal structure of probable enoyl-coa hydratase from mycob smegmatis
Source: Mycobacterium smegmatis. Organism_taxid: 246196. Strain: atcc 700084/ mc(2)155. Gene: msmeg_6777. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (258 residues) CATH domains: 3.90.226.10 1.10.12.10
3myb
Crystal structure of enoyl-coa hydratase mycobacterium smegm
Source: Mycobacterium smegmatis. Organism_taxid: 246196. Strain: atcc 700084/mc(2)155. Gene: msmeg_1594. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C (259 residues) CATH domains: 3.90.226.10 1.10.12.10
3njb
Crystal structure of enoyl-coa hydratase from mycobacterium iodide soak
Source: Mycobacterium smegmatis. Organism_taxid: 246196. Strain: atcc 700084 / mc(2)155. Gene: msmeg_1388. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (290 residues) CATH domain: 3.90.226.10
3njd
Crystal structure of enoyl-coa hydratase from mycobacterium
Source: Mycobacterium smegmatis. Organism_taxid: 246196. Strain: atcc 700084 / mc(2)155. Gene: msmeg_1388. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (290 residues) CATH domain: 3.90.226.10
3ome
Crystal structure of a probable enoyl-coa hydratase from myc smegmatis
Source: Mycobacterium smegmatis. Organism_taxid: 246196. Strain: mc2 155. Gene: msmeg_4846. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D, E, F (243 residues) CATH domain: 3.90.226.10
3pzk
Crystal structure of the mycobacterium tuberculosis crotonas form
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Strain: h37rv. Gene: echa8, mt1100, mtv017.23c, rv1070c. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C (244 residues) CATH domains: 3.90.226.10 1.10.12.10
3q0g
Crystal structure of the mycobacterium tuberculosis crotonas a reaction intermediate derived from crotonyl coa
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Gene: echa8, mt1100, mtv017.23c, rv1070c. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D, E, F (252 residues) CATH domains: 3.90.226.10 1.10.12.10
Bound ligand:   Het Group BCO is 94.55% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
3q0j
Crystal structure of the mycobacterium tuberculosis crotonas complex with the inhibitor acetoacetylcoa
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Gene: echa8, mt1100, mtv017.23c, rv1070c. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D, E, F (255 residues) CATH domains: 3.90.226.10 1.10.12.10
Bound ligand:   Het Group CAA is 96.36% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
3zw8
Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) in apo form
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: peroxisome. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (725 residues) CATH domains: 3.90.226.10 3.40.50.720
3zw9
Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2- methylbutanoyl-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: peroxisome. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (723 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group T1G is 94.64% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
3zwa
Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-hexanoyl-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: peroxisome. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (727 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group 3H9 is 92.98% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
3zwb
Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 2trans-hexenoyl-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: peroxisome. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (725 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group TC6 is 91.23% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
3zwc
Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: peroxisome. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (725 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group HSC is 86.89% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
4fjw
Crystal structure of the apo form of the e131q mtb crotonase
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Strain: h37rv. Gene: echa8, mt1100, mtv017.23c, rv1070c. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: D, E, F (256 residues) CATH domains: 3.90.226.10 1.10.12.10
4fzw
Crystal structure of the paaf-paag hydratase-isomerase compl e.Coli
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b1393, jw1388, paaf, ydbr. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: paag, ydbt, b1394, jw1389.
Chains: A, B (254 residues) CATH domains: 3.90.226.10 1.10.12.10
4j2u
Crystal structure of an enoyl-coa hydratase from rhodobacter sphaeroides 2.4.1
Source: Rhodobacter sphaeroides. Organism_taxid: 272943. Strain: 2.4.1. Gene: atcc baa-808, rhos4_17590, rsp_0155. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (344 residues) CATH domain: Unassigned
4jcs
Crystal structure of enoyl-coa hydratase/isomerase from cupr metallidurans ch34
Source: Cupriavidus metallidurans. Organism_taxid: 266264. Strain: ch34 / atcc 43123 / dsm 2839. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (265 residues) CATH domains: 3.90.226.10 1.10.12.10
4jfc
Crystal structure of a enoyl-coa hydratase from polaromonas
Source: Polaromonas sp. Js666. Organism_taxid: 296591. Strain: js666. Gene: bpro_2958. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (257 residues) CATH domain: 3.90.226.10
4jsb
Crystal structure of tfu_1878, a putative enoyl-coa hydratas thermobifida fusca yx
Source: Thermobifida fusca. Organism_taxid: 269800. Strain: yx. Gene: tfu_1878. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (260 residues) CATH domain: 3.90.226.10
4jvt
Crystal structure of tfu_1878, a putative enoyl-coa hydratas fromthermobifida fusca yx in complex with coa
Source: Thermobifida fusca. Organism_taxid: 269800. Strain: yx. Gene: tfu_1878. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (257 residues) CATH domain: 3.90.226.10
Bound ligand:   Het Group ACO is 88.89% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
4jwv
Crystal structure of putative short chain enoyl-coa hydratas novosphingobium aromaticivorans dsm 12444
Source: Novosphingobium aromaticivorans. Organism_taxid: 279238. Strain: dsm 12444. Gene: saro_1463. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (254 residues) CATH domains: 3.90.226.10 1.10.12.10
4omr
Crystal structure of tfu_1878, a putative enoyl-coa hydratas thermobifida fusca yx in complex with acetoacetyl-coa
Source: Thermobifida fusca. Organism_taxid: 269800. Strain: yx. Gene: tfu_1878. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (255 residues) CATH domain: 3.90.226.10
Bound ligand:   Het Group CAA is 96.36% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
4qfe
Crystal structure of an enoyl-coa hydratase from mycobacteri smegmatis
Source: Mycobacterium smegmatis. Organism_taxid: 246196. Strain: atcc 700084 / mc(2)155. Gene: echa5, msmeg_1390, msmei_1352. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (225 residues) CATH domain: 3.90.226.10