_EC 3.6.4.6 Vesicle-fusing ATPase. 34 PDB entries  
EC 3.-.-.- Hydrolases. [20,887 PDB entries]
EC 3.6.-.- Acting on acid anhydrides. [2,188 PDB entries]
EC 3.6.4.- Acting on acid anhydrides; involved in cellular and subcellular m [844 PDB entries]
EC 3.6.4.6 Vesicle-fusing ATPase. [34 PDB entries]    
1d2n

Reaction: Atp + H(2)O = adp + phosphate.
 


ATP
+ H(2)O
=
ADP
+
phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Comments: A large family of ATP-hydrolyzing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger's syndrome.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 34 PDB entries in enzyme class E.C.3.6.4.6

  PDB code Protein
1d2n
D2 domain of n-ethylmaleimide-sensitive fusion protein
Source: Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Organ: ovary. Cellular_location: cytoplasm. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (246 residues) CATH domains: 3.40.50.300 1.10.8.60
Bound ligand:   Het Group ANP is 81.25% similar to enzyme product ADP
1e32
Structure of the n-terminal domain and the d1 aaa domain of membrane fusion atpase p97
Source: Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (438 residues) CATH domains: 2.40.40.20 3.40.50.300 3.10.330.10 1.10.8.60
1nsf
D2 hexamerization domain of n-ethylmaleimide sensitive facto
Source: Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Cell_line: cho. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (247 residues) CATH domains: 3.40.50.300 1.10.8.60
Bound ligand:   Het Group ATP corresponds to enzyme reactant ATP
1qcs
N-terminal domain of n-ethylmaleimide sensitive factor (nsf)
Source: Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Tissue: ovary. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (195 residues) CATH domains: 2.40.40.20 3.10.330.10
1qdn
Amino terminal domain of the n-ethylmaleimide sensitive fusion protein (nsf)
Source: Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Organ: ovary. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (189 residues) CATH domains: 2.40.40.20 3.10.330.10
1r7r
The crystal structure of murine p97/vcp at 3.6a
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (683 residues) CATH domains: 2.40.40.20 3.10.330.10 3.40.50.300 1.10.8.60 3.40.50.300 1.10.8.60
1s3s
Crystal structure of aaa atpase p97/vcp nd1 in complex with p47 c
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562. Rattus norvegicus. Norway rat. Organism_taxid: 10116.
Chains: A, B, C, D, E, F (436 residues) CATH domains: 2.40.40.20 3.40.50.300 3.10.330.10 1.10.8.60
1wr0
Structural characterization of the mit domain from human vps4b
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (81 residues) CATH domain: 1.20.58.280
1xwi
Crystal structure of vps4b
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vps4b, skd1, vps42. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (322 residues) CATH domain: 3.40.50.300
1yxr
Nmr structure of vps4a mit domain
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: 27183. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693. Other_details: pet16b reengineered to contain a tev cleavage site in place of the normal factor xa site
Chain: A (77 residues) CATH domain: 1.20.58.280
2cpt
Solution structure of mit domain from human skd1
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vps4b, skd1, vps42. Other_details: cell free protein synthesis
Chain: A (117 residues) CATH domain: 1.20.58.280
2jq9
Vps4a mit-chmp1a complex
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vps4a, vps4. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: pcoln3, chmp1, chmp1a, kiaa0047, prsm1.
Chain: A (71 residues) CATH domain: 1.20.58.280
2jqh
Vps4b mit
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vps4b, skd1, vps42. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (72 residues) CATH domain: 1.20.58.280
2jqk
Vps4b mit-chmp2b complex
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vps4b, skd1, vps42. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: chmp2b.
Chain: A (72 residues) CATH domain: 1.20.58.280
2k3w
Nmr structure of vps4a-mit-chmp6
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vps4a, vps4. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: chmp6, vps20.
Chain: A (73 residues) CATH domain: 1.20.58.280
2pjh
Strctural model of the p97 n domain- npl4 ubd complex
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: p97. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693. Gene: npl4. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: B (193 residues) CATH domains: 2.40.40.20 3.10.330.10
2zam
Crystal structure of mouse skd1/vps4b apo-form
Source: Mus musculus. Mouse. Organism_taxid: 10090. Gene: skd1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (308 residues) CATH domain: 3.40.50.300
2zan
Crystal structure of mouse skd1/vps4b atp-form
Source: Mus musculus. Mouse. Organism_taxid: 10090. Gene: skd1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (312 residues) CATH domain: 3.40.50.300
Bound ligand:   Het Group ATP corresponds to enzyme reactant ATP
2zao
Crystal structure of mouse skd1/vps4b adp-form
Source: Mus musculus. Mouse. Organism_taxid: 10090. Gene: skd1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (312 residues) CATH domain: 3.40.50.300
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
3cf0
Structure of d2 subdomain of p97/vcp in complex with adp
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N (281 residues) CATH domain: 3.40.50.300
3cf1
Structure of p97/vcp in complex with adp/adp.Alfx
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (719 residues)
3cf2
Structure of p97/vcp in complex with adp/amp-pnp
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (659 residues) CATH domains: 2.40.40.20 3.10.330.10 3.40.50.300 1.10.8.60 3.40.50.300
3cf3
Structure of p97/vcp in complex with adp
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (723 residues)
3hu1
Structure of p97 n-d1 r95g mutant in complex with atpgs
Source: Homo sapiens. Human. Organism_taxid: 9606. Strain: human. Gene: p97, vcp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (451 residues) CATH domains: 2.40.40.20 3.10.330.10 3.40.50.300 1.10.8.60
3hu2
Structure of p97 n-d1 r86a mutant in complex with atpgs
Source: Homo sapiens. Human. Organism_taxid: 9606. Strain: human. Gene: p97, vcp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (452 residues) CATH domains: 2.40.40.20 3.10.330.10 3.40.50.300 1.10.8.60
3hu3
Structure of p97 n-d1 r155h mutant in complex with atpgs
Source: Homo sapiens. Human. Organism_taxid: 9606. Strain: human. Gene: p97, vcp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (453 residues) CATH domains: 2.40.40.20 3.10.330.10 3.40.50.300 1.10.8.60
3qc8
Crystal structure of faf1 ubx domain in complex with p97/vcp reveals the conserved fcisp touch-turn motif of ubx domain conformational change
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: faf1. Expression_system_taxid: 562
Chain: A (170 residues) CATH domains: 2.40.40.20 3.10.330.10
3qq7
Crystal structure of the p97 n-terminal domain
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (168 residues) CATH domains: 2.40.40.20 3.10.330.10
3qq8
Crystal structure of p97-n in complex with faf1-ubx
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: faf1, ubxd12, ubxn3a, cgi-03. Expression_system_taxid: 562
Chain: A (177 residues) CATH domains: 2.40.40.20 3.10.330.10
3qwz
Crystal structure of faf1 ubx-p97n-domain complex
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: faf1, ubxd12, ubxn3a, cgi-03.
Chain: A (177 residues) CATH domains: 2.40.40.20 3.10.330.10
3tiw
Crystal structure of p97n in complex with thE C-terminus of
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 9606
Chains: A, B (160 residues) CATH domains: 2.40.40.20 3.10.330.10
4kln
Structure of p97 n-d1 a232e mutant in complex with atpgs
Source: Homo sapiens. Human. Organism_taxid: 9606. Strain: human. Gene: p97, vcp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (451 residues) CATH domains: 2.40.40.20 3.10.330.10 3.40.50.300 1.10.8.60
Bound ligand:   Het Group AGS is 93.75% similar to enzyme reactant ATP
4ko8
Structure of p97 n-d1 r155h mutant in complex with atpgs
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: p97, vcp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (456 residues) CATH domains: 2.40.40.20 3.10.330.10 3.40.50.300 1.10.8.60
Bound ligand:   Het Group AGS is 93.75% similar to enzyme reactant ATP
4kod
Structure of p97 n-d1 r155h mutant in complex with adp
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: p97, vcp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (438 residues) CATH domains: 2.40.40.20 3.10.330.10 3.40.50.300 1.10.8.60
Bound ligand:   Het Group ADP corresponds to enzyme product ADP