_EC 3.5.1.28 N-acetylmuramoyl-L-alanine amidase. 34 PDB entries  
EC 3.-.-.- Hydrolases. [22,314 PDB entries]
EC 3.5.-.- Acting on carbon-nitrogen bonds, other than peptide bonds. [1,811 PDB entries]
EC 3.5.1.- In linear amides. [633 PDB entries]
EC 3.5.1.28 N-acetylmuramoyl-L-alanine amidase. [34 PDB entries]    
1aro

Reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Comments: Formerly Ec 3.4.12.5, Ec 3.4.17.7 and Ec 3.4.19.10.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 34 PDB entries in enzyme class E.C.3.5.1.28

  PDB code Protein
1aro
T7 RNA polymerase complexed with t7 lysozyme
Source: Enterobacteria phage t7. Organism_taxid: 10760. Gene: t7. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: L (149 residues) CATH domain: 3.40.80.10
1gvm
Choline binding domain of the major autolysin (c-lyta) from streptococcus pneumoniae
Source: Streptococcus pneumoniae. Pneumococcus. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (127 residues) CATH domain: 2.10.270.10
1h8g
C-terminal domain of the major autolysin (c-lyta) from streptococcus pneumoniae
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Cellular_location: extracellular. Gene: lyta. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chains: A, B (95 residues) CATH domain: 2.10.270.10
1hcx
Choline binding domain of the major autolysin (c-lyta) from streptococcus pneumoniae
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Cellular_location: extracellular. Gene: lyta. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (127 residues) CATH domain: 2.10.270.10
1iya
Solution structure of citrobacter freundii ampd
Source: Citrobacter freundii. Bacteria. Expressed in: escherichia coli.
Chain: A (187 residues) CATH domain: 3.40.80.10
1j2s
Nmr structure of citrobacter freundii ampd
Source: Citrobacter freundii. Bacteria. Gene: ampd. Expressed in: escherichia coli.
Chain: A (187 residues) CATH domain: 3.40.80.10
1j3g
Solution structure of citrobacter freundii ampd
Source: Citrobacter freundii. Organism_taxid: 546. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (187 residues) CATH domain: 3.40.80.10
1lba
The structure of bacteriophage t7 lysozyme, a zinc amidase and an inhibitor of t7 RNA polymerase
Source: Enterobacteria phage t7. Organism_taxid: 10760. Gene: t7.
Chain: A (146 residues) CATH domain: 3.40.80.10
1oht
Peptidoglycan recognition protein-lb
Source: Drosophila melanogaster. Organism_taxid: 7227. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (174 residues) CATH domain: 3.40.80.10
1x60
Solution structure of the peptidoglycan binding domain of b. Subtilis cell wall lytic enzyme cwlc
Source: Bacillus subtilis. Organism_taxid: 1423. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (79 residues) CATH domain: 3.30.70.1070
1yb0
Structure of plyl
Source: Bacillus anthracis. Organism_taxid: 1392. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B, C (157 residues) CATH domain: 3.40.80.10
2ar3
E90a mutant structure of plyl
Source: Bacillus anthracis. Organism_taxid: 1392. Gene: sterne. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B, C (157 residues) CATH domain: 3.40.80.10
2bh7
Crystal structure of a semet derivative of amid at 2.2 angstroms
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12 mg1655
Chain: A (254 residues) CATH domains: Unassigned 3.40.80.10 1.10.101.10
2bml
Ofloxacin-like antibiotics inhibit pneumococcal cell wall degrading virulence factors
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (125 residues) CATH domain: 2.10.270.10
2kq8
Solution nmr structure of a domain from bt9727_4915 from bacillus thuringiensis, northeast structural genomics consortium target bur95a
Source: Bacillus thuringiensis serovar konkukian. Organism_taxid: 180856. Gene: bt9727_4915. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (70 residues)
2wkx
Crystal structure of the native e. Coli zinc amidase amid
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12 mg1655. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (257 residues) CATH domains: 3.40.80.10 1.10.101.10
2y28
Crystal structure of se-met ampd derivative
Source: Citrobacter freundii. Organism_taxid: 546. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (180 residues) CATH domain: 3.40.80.10
2y2b
Crystal structure of ampd in complex with reaction products
Source: Citrobacter freundii. Organism_taxid: 546. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (179 residues) CATH domain: 3.40.80.10
2y2c
Crystal structure of ampd apoenzyme
Source: Citrobacter freundii. Organism_taxid: 546. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (179 residues) CATH domain: 3.40.80.10
2y2d
Crystal structure of ampd holoenzyme
Source: Citrobacter freundii. Organism_taxid: 546. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (179 residues) CATH domain: 3.40.80.10
2y2e
Crystal structure of ampd grown at ph 5.5
Source: Citrobacter freundii. Organism_taxid: 546. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (180 residues) CATH domain: 3.40.80.10
3d2y
Complex of the n-acetylmuramyl-l-alanine amidase amid from e the substrate anhydro-n-acetylmuramic acid-l-ala-d-gamma-gl
Source: Escherichia coli. Organism_taxid: 511145. Strain: k-12 mg1655. Gene: amid, ybjr, b0867, jw0851. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: the peptide was chemically synthesized
Chain: A (257 residues) CATH domains: 3.40.80.10 1.10.101.10
3d2z
Complex of the n-acetylmuramyl-l-alanine amidase amid from e the product l-ala-d-gamma-glu-l-lys
Source: Escherichia coli str. K12 substr. Mg16 organism_taxid: 511145. Strain: k-12 mg1655. Gene: amid, ybjr, b0867, jw0851. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: the peptide was chemically synthesized
Chain: A (256 residues) CATH domains: 3.40.80.10 1.10.101.10
3hma
Amidase from bacillus subtilis
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: xlya, bsu12810. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (153 residues) CATH domain: 3.40.80.10
3hmb
Mutant endolysin from bacillus subtilis
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: xlya, bsu12810. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (154 residues) CATH domain: 3.40.80.10
3lat
Crystal structure of staphylococcus peptidoglycan hydrolase amie
Source: Staphylococcus epidermidis. Organism_taxid: 1282. Strain: o-47. Gene: atle. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (207 residues) CATH domain: Unassigned
3rdr
Structure of the catalytic domain of xlya
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: xlya, bsu12810. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (153 residues) CATH domain: 3.40.80.10
4bin
Crystal structure of the e. Coli n-acetylmuramoyl-l-alanine amic
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Atcc: 10798
Chain: A (348 residues) CATH domains: Unassigned Unassigned
4epc
Crystal structure of autolysin repeat domains from staphyloc epidermidis
Source: Staphylococcus epidermidis. Organism_taxid: 1282. Gene: atl, atle. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (152 residues) CATH domains: Unassigned 2.30.30.170
4ivv
Catalytic amidase domain of the major autolysin lyta from streptococcus pneumaniae
Source: Streptococcus pneumoniae. Organism_taxid: 170187. Strain: strain atcc baa-334 / tigr4. Gene: lyta, sp_1937. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (174 residues) CATH domain: 3.40.80.10
4iwt
Crystal structure of thE C-teminal choline-binding domain of streptococcus pneumoniae prophage lyta
Source: Streptococcus pneumoniae. Organism_taxid: 1105119. Strain: atcc 700673. Gene: lyta. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (146 residues)
4knk
Crystal structure of staphylococcus aureus hydrolase amia
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 93061. Strain: nctc 8325. Gene: atl, NAG, saouhsc_00994. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (206 residues) CATH domain: Unassigned
4knl
Crystal structure of staphylococcus aureus hydrolase amia in with its ligand
Source: Staphylococcus aureus subsp. Aureus. Organism_taxid: 93061. Strain: nctc 8325. Gene: atl, NAG, saouhsc_00994. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chains: A, B, C, D (205 residues) CATH domain: Unassigned
4rn7
The crystal structure of n-acetylmuramoyl-l-alanine amidase clostridium difficile 630
Source: Peptoclostridium difficile. Organism_taxid: 272563. Strain: 630. Gene: cd630_27610. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (186 residues) CATH domain: 3.40.630.40