_EC 3.4.22.68 Ulp1 peptidase. 24 PDB entries  
EC 3.-.-.- Hydrolases. [21,057 PDB entries]
EC 3.4.-.- Acting on peptide bonds (peptide hydrolases). [6,846 PDB entries]
EC 3.4.22.- Cysteine endopeptidases. [986 PDB entries]
EC 3.4.22.68 Ulp1 peptidase. [24 PDB entries]    
1euv

Reaction: Hydrolysis of the alpha-linked peptide bond in the sequence Gly- Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature Sumo and the lysine epsilon-amino group of the target protein.
Other name(s): Smt3-protein conjugate proteinase. Ubl-specific protease 1. Ulp1. Ulp1 endopeptidase. Ulp1 protease.
Comments: The enzyme from Saccharomyces cerevisiae can also recognize small ubiquitin-like modifier 1 (SUMO-1) from human as a substrate in both SUMO-processing (alpha-linked peptide bonds) and SUMO-deconjugation (epsilon-linked peptide bonds) reactions. Ulp1 has several functions, including an essential role in chromosomal segregation and progression of the cell cycle through the G2/M phase of the cell cycle. Belongs to peptidase family C48.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 24 PDB entries in enzyme class E.C.3.4.22.68

  PDB code Protein
1euv
X-ray structure of thE C-terminal ulp1 protease domain in complex with smt3, the yeast ortholog of sumo.
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (221 residues) CATH domains: Unassigned 3.30.310.130
1tgz
Structure of human senp2 in complex with sumo-1
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: senp2, kiaa1331. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ubl1, smt3h3, smt3c.
Chain: A (224 residues) CATH domain: Unassigned
1th0
Structure of human senp2
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: senp2, kiaa1331. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (226 residues) CATH domain: Unassigned
1xt9
Crystal structure of den1 in complex with nedd8
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: senp8, fksg8, prsc2. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: glutathione s-transferase fusion, cleaved with thrombin. Gene: nedd8.
Chain: A (208 residues)
2bkq
Nedd8 protease
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D (211 residues)
2bkr
Nedd8 nedp1 complex
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (212 residues)
2ckg
The structure of senp1 sumo-2 co-complex suggests a structural basis for discrimination between sumo paralogues during processing
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (225 residues) CATH domain: Unassigned
2ckh
Senp1-sumo2 complex
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (225 residues) CATH domain: Unassigned
2g4d
Crystal structure of human senp1 mutant (c603s) in complex with sumo-1
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chains: A, C (205 residues) CATH domain: Unassigned
2hkp
Sumo protease ulp1 with the catalytic cysteine oxidized to a sulfenic acid
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (219 residues) CATH domains: Unassigned 3.30.310.130
2hl8
Sumo protease ulp1 with the catalytic cysteine oxidized to a sulfinic acid
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (219 residues) CATH domains: Unassigned 3.30.310.130
2hl9
Sumo protease ulp1 with the catalytic cysteine oxidized to a sulfonic acid
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (218 residues) CATH domains: Unassigned 3.30.310.130
2io0
Crystal structure of human senp2 in complex with presumo-2
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: senp2, kiaa1331. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: sumo2, smt3b, smt3h2.
Chain: A (226 residues) CATH domain: Unassigned
2io1
Crystal structure of human senp2 in complex with presumo-3
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: senp2, kiaa1331. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: sumo3, smt3a, smt3h1.
Chains: A, C, E (223 residues) CATH domain: Unassigned
2io2
Crystal structure of human senp2 in complex with rangap1-sum
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: senp2, kiaa1331. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: sumo1, smt3c, smt3h3, ubl1. Gene: rangap1.
Chain: A (225 residues) CATH domain: Unassigned
2io3
Crystal structure of human senp2 in complex with rangap1- sumo-2
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: senp2, kiaa1331. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: sumo2, smt3b, smt3h2. Gene: rangap1.
Chain: A (224 residues) CATH domain: Unassigned
2iy0
Senp1 (mutant) sumo1 rangap
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chain: A (226 residues) CATH domain: Unassigned
2iy1
Senp1 (mutant) full length sumo1
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chains: A, C (226 residues) CATH domain: Unassigned
2iyc
Senp1 native structure
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (226 residues) CATH domain: Unassigned
2iyd
Senp1 covalent complex with sumo-2
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chain: A (226 residues) CATH domain: Unassigned
2xph
Crystal structure of human senp1 with the bound cobalt
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (227 residues) CATH domain: Unassigned
2xre
Detection of cobalt in previously unassigned human senp1 str
Source: Homo sapiens. Human. Organism_taxid: 9606. Cell_line: escherichia coli. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: gold.
Chains: A, B (227 residues) CATH domain: Unassigned
3eay
Crystal structure of the human senp7 catalytic domain
Source: Homo sapiens. Organism_taxid: 9606. Gene: senp7, kiaa1707, ssp2, susp2. Expressed in: escherichia coli.
Chain: A (247 residues) CATH domains: Unassigned Unassigned
3zo5
Structure of senp2-loop1 in complex with presumo-2
Source: Homo sapiens. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (230 residues) CATH domain: Unassigned