_EC 3.4.21.12 Alpha-lytic endopeptidase. 48 PDB entries  
EC 3.-.-.- Hydrolases. [21,051 PDB entries]
EC 3.4.-.- Acting on peptide bonds (peptide hydrolases). [6,852 PDB entries]
EC 3.4.21.- Serine endopeptidases. [2,536 PDB entries]
EC 3.4.21.12 Alpha-lytic endopeptidase. [48 PDB entries]    
1boq

Reaction: Preferential cleavage: Ala-|-Xaa, Val-|-Xaa in bacterial cell walls, elastin and other proteins.
Other name(s): Alpha-lytic protease.
Comments: Belongs to peptidase family S1E.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 48 PDB entries in enzyme class E.C.3.4.21.12

  PDB code Protein
1boq
Pro region c-terminus: protease active site interactions are critical in catalyzing the folding of alpha-lytic protease
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Collection: 29487
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gba
Alpha-lytic protease with met 190 replaced by ala and gly 216 replaced by ala
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbb
Alpha-lytic protease with met 190 replaced by ala and gly 21 by ala complex with methoxysuccinyl-ala-ala-pro-alanine bor
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbc
Alpha-lytic protease with met 190 replaced by ala and gly 21 by ala complex with methoxysuccinyl-ala-ala-pro-leucine bor
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbd
Alpha-lytic protease with met 190 replaced by ala and gly 21 by ala complex with methoxysuccinyl-ala-ala-pro-phenylalani acid
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbe
Alpha-lytic protease with met 190 replaced by ala and gly 216 replaced by leu
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbf
Alpha-lytic protease with met 190 replaced by ala and gly 21 by leu complex with methoxysuccinyl-ala-ala-pro-alanine bor
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbh
Alpha-lytic protease with met 190 replaced by ala and gly 21 by leu complex with methoxysuccinyl-ala-ala-pro-leucine bor
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbi
Alpha-lytic protease with met 190 replaced by ala and gly 21 by leu complex with methoxysuccinyl-ala-ala-pro-phenylalani acid
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbj
Alpha-lytic protease with met 190 replaced by ala
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbk
Alpha-lytic protease with met 190 replaced by ala complex wi methoxysuccinyl-ala-ala-pro-alanine boronic acid
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbl
Alpha-lytic protease with met 190 replaced by ala complex wi methoxysuccinyl-ala-ala-pro-leucine boronic acid
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1gbm
Alpha-lytic protease with met 190 replaced by ala complex wi methoxysuccinyl-ala-ala-pro-phenylalanine boronic acid
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Gene: alpha-lytic protease preproenz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p01
Serine protease mechanism. Structure of an inhibitory comple alpha-lytic protease and a tightly bound peptide boronic ac
Source: Lysobacter enzymogenes. Organism_taxid: 69. Gene: alpha-lp
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p02
Structure analysis of specificity. Alpha-lytic protease comp analogues of reaction intermediates
Source: Lysobacter enzymogenes. Organism_taxid: 69. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p03
Structure analysis of specificity. Alpha-lytic protease comp analogues of reaction intermediates
Source: Lysobacter enzymogenes. Organism_taxid: 69. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p04
Structure analysis of specificity. Alpha-lytic protease comp analogues of reaction intermediates
Source: Lysobacter enzymogenes. Organism_taxid: 69. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p05
Structure analysis of specificity. Alpha-lytic protease comp analogues of reaction intermediates
Source: Lysobacter enzymogenes. Organism_taxid: 69. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p06
Structure analysis of specificity. Alpha-lytic protease comp analogues of reaction intermediates
Source: Lysobacter enzymogenes. Organism_taxid: 69. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p09
Structural plasticity as a determinant of enzyme specificity broadly specific proteases
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p10
Structural plasticity as a determinant of enzyme specificity broadly specific proteases
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p11
Crystal structures of alpha-lytic protease complexes with ir bound phosphonate esters
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: E (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1p12
Crystal structures of alpha-lytic protease complexes with ir bound phosphonate esters
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: E (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1qq4
Crystal structure of an alpha-lytic protease mutant with acc folding kinetics, r102h/g134s
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1qrw
Crystal structure of an alpha-lytic protease mutant with acc folding kinetics, r102h/g134s, ph 8
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1qrx
Crystal structure of wild-type alpha-lytic protease at 1.6 a
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1ssx
0.83a resolution crystal structure of alpha-lytic protease a
Source: Lysobacter enzymogenes. Organism_taxid: 69. Gene: alpha-lp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
1tal
Alpha-lytic protease at 120 k (single structure model)
Source: Lysobacter enzymogenes. Organism_taxid: 69. Strain: 495. Atcc: 29487. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
2alp
Refined structure of alpha-lytic protease at 1.7 angstroms resolution. Analysis of hydrogen bonding and solvent structure
Source: Lysobacter enzymogenes. Organism_taxid: 69
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
2h5c
0.82a resolution crystal structure of alpha-lytic protease a
Source: Lysobacter enzymogenes. Organism_taxid: 69. Secretion: secreted by the native bacterium. Other_details: gene alpha-lp
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
2h5d
0.9a resolution crystal structure of alpha-lytic protease co with a transition state analogue, meosuc-ala-ala-pro-val bo
Source: Lysobacter enzymogenes. Organism_taxid: 69. Secretion: secreted by the native bacterium. Other_details: gene alpha-lp. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
2lpr
Structural basis for broad specificity in alpha-lytic protea
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
2pro
Pro region of alpha-lytic protease
Source: Lysobacter enzymogenes. Organism_taxid: 69. Cell_line: b834. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: t7 expression system
Chains: A, B, C (142 residues) CATH domains: 3.30.300.50 3.30.300.50
2ull
Multiple conformation structure of alpha-lytic protease at 120 k
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
3lpr
Structural basis for broad specificity in alpha-lytic protea
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
3m7t
Crystal structure of alpha-lytic protease sb2+3 e8a/r105s mu
Source: Lysobacter enzymogenes. Organism_taxid: 69. Gene: alpha-lp, lysobacter. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
3m7u
Crystal structure of alpha-lytic protease sb1+2 r64a/e182q m
Source: Lysobacter enzymogenes. Organism_taxid: 69. Gene: alpha-lp, lysobacter. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
3pro
Alpha-lytic protease complexed with c-terminal truncated pro
Source: Lysobacter enzymogenes. Organism_taxid: 69. Cell_line: b834. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: t7 expression system
Chains: A, C, B, D (198 residues) CATH domains: 2.40.10.10 2.40.10.10
3qgj
1.3a structure of alpha-lytic protease bound to ac-alaalapro
Source: Lysobacter enzymogenes. Organism_taxid: 69. Synthetic: yes. Other_details: synthesized by commonly used techniques, in
Chains: A, C (198 residues) CATH domains: 2.40.10.10 2.40.10.10
3urc
T181g mutant of alpha-lytic protease
Source: Lysobacter enzymogenes. Organism_taxid: 69. Gene: alpha-lp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
3urd
T181a mutant of alpha-lytic protease
Source: Lysobacter enzymogenes. Organism_taxid: 69. Gene: alpha-lp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
3ure
Repack mutant (t181i, w199l, q210i) of alpha-lytic protease
Source: Lysobacter enzymogenes. Organism_taxid: 69. Gene: alpha-lp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (198 residues) CATH domains: 2.40.10.10 2.40.10.10
4pro
Alpha-lytic protease complexed with pro region
Source: Fragment: chain c, d. Lysobacter enzymogenes. Organism_taxid: 69. Gene: t7. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, C, B, D (198 residues) CATH domains: 2.40.10.10 2.40.10.10
5lpr
Structural basis for broad specificity in alpha-lytic protea
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
6lpr
Structural basis for broad specificity in alpha-lytic protea
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
7lpr
Structural basis for broad specificity in alpha-lytic protea
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
8lpr
Structural basis for broad specificity in alpha-lytic protea
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10
9lpr
Structural basis for broad specificity in alpha-lytic protea
Source: Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (198 residues) CATH domains: 2.40.10.10 2.40.10.10