_EC 3.4.21.107 Peptidase Do. 40 PDB entries  
EC 3.-.-.- Hydrolases. [21,795 PDB entries]
EC 3.4.-.- Acting on peptide bonds (peptide hydrolases). [7,039 PDB entries]
EC 3.4.21.- Serine endopeptidases. [2,574 PDB entries]
EC 3.4.21.107 Peptidase Do. [40 PDB entries]    
1ky9

Reaction: Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.
Other name(s): High temperature requirement protease A. HrtA heat shock protein. Protease Do.
Comments: This serine endopeptidase is essential for the clearance of denatured or aggregated proteins from the inner-membrane and periplasmic space in Escherichia coli. Natural substrates of the enzyme include colicin A lysis protein, pilin subunits and MalS from E.coli. The enzyme has weak peptidase activity with casein and other non- native substrates. The peptidase acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures. Molecular chaperones and peptidases control the folded state of
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 40 PDB entries in enzyme class E.C.3.4.21.107

  PDB code Protein
1ky9
Crystal structure of degp (htra)
Source: Escherichia coli. Organism_taxid: 562. Gene: degp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (311 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10
1sot
Crystal structure of the degs stress sensor
Source: Escherichia coli. Organism_taxid: 562. Gene: degs, hhob, htrh, b3235, z4594, ecs4108. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B, C (283 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10
1soz
Crystal structure of degs protease in complex with an activating peptide
Source: Escherichia coli. Organism_taxid: 562. Gene: degs, hhob, htrh, b3235, z4594, ecs4108. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes
Chains: A, B, C (281 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10
1te0
Structural analysis of degs, a stress sensor of the bacteria periplasm
Source: Escherichia coli. Organism_taxid: 562. Gene: degs, hhob, htrh, b3235, z4594, ecs4108. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (318 residues) CATH domains: 2.40.10.10 2.40.10.10
1vcw
Crystal structure of degs after backsoaking the activating peptide
Source: Escherichia coli. Organism_taxid: 562. Gene: degs, hhob, htrh, b3235, z4594, ecs4108. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B, C (291 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10
2qf0
Structure of the delta pdz truncation of the degs protease
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degs, hhob, htrh. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H, I (202 residues) CATH domains: 2.40.10.10 2.40.10.10
2qf3
Structure of the delta pdz truncation of the degs protease
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degs, hhob, htrh. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (200 residues) CATH domains: 2.40.10.10 2.40.10.10
2qgr
Structure of the r178a mutant of delta pdz degs protease
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degs, hhob, htrh. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (169 residues) CATH domains: 2.40.10.10 2.40.10.10
2r3u
Crystal structure of the pdz deletion mutant of degs
Source: Escherichia coli. Organism_taxid: 562. Gene: degs, hhob, htrh. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B, C (202 residues) CATH domains: 2.40.10.10 2.40.10.10
2r3y
Crystal structure of the degs protease in complex with the ywf activating peptide
Source: Escherichia coli. Organism_taxid: 562. Gene: degs, hhob, htrh. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Other_details: the ywf peptide mimics thE C-terminus of outer membrane proteins.
Chains: A, B, C (280 residues) CATH domains: 2.40.10.10 2.40.10.10
2rce
Dfp modified degs delta pdz
Source: Escherichia coli. Organism_taxid: 562. Gene: degs, hhob, htrh. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I (205 residues) CATH domains: 2.40.10.10 2.40.10.10
2zle
Cryo-em structure of degp12/omp
Source: Escherichia coli. Organism_taxid: 562. Gene: degp, htra, ptd. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ompc, meoa, par. Expression_system_taxid: 562
Chains: A, B, C, E, F, G, H, I, J, K, L, M (396 residues)
3b8j
Q191a mutant of degs-deltapdz
Source: Escherichia coli. Strain: k12. Gene: degs, hhob, htrh. Expressed in: escherichia coli.
Chain: A (184 residues) CATH domains: 2.40.10.10 2.40.10.10
3cs0
Crystal structure of degp24
Source: Escherichia coli. Gene: degp, htra, ptd. Expressed in: escherichia coli. Synthetic: yes
Chain: A (392 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10 2.30.42.10
3gcn
Crystal structure of degs h198p/d320a mutant modified by dfp complex with omp peptide (yqf)
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (301 residues) CATH domains: 2.40.10.10 2.40.10.10
3gco
Crystal structure of degs h198p/d320a mutant modified by dfp complex with dnrdgnvyqf omp peptide
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (303 residues) CATH domains: 2.40.10.10 2.40.10.10
3gds
Crystal structure of degs h198p/d320a mutant modified by dfp complex with dnrdgnvyyf peptide
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: A (296 residues) CATH domains: 2.40.10.10 2.40.10.10
3gdu
Crystal structure of degs h198p/d320a mutant modified by dfp and in complex with yrf peptide
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chains: A, B, C (295 residues) CATH domains: 2.40.10.10 2.40.10.10
3gdv
Crystal structure of degs h198p/d320a mutant modified by dfp and in complex with yqf peptide
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chains: A, B, C (296 residues) CATH domains: 2.40.10.10 2.40.10.10
3lgi
Structure of the protease domain of degs (degs-deltapdz) at
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (220 residues) CATH domains: 2.40.10.10 2.40.10.10
3lgt
Y162a/h198p double mutant of degs-deltapdz protease
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (181 residues) CATH domains: 2.40.10.10 2.40.10.10
3lgu
Y162a mutant of the degs-deltapdz protease
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (191 residues) CATH domains: 2.40.10.10 2.40.10.10
3lgv
H198p mutant of the degs-deltapdz protease
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H, I (204 residues) CATH domains: 2.40.10.10 2.40.10.10
3lgw
H198p/t167v double mutant of degs-deltapdz protease
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (187 residues) CATH domains: 2.40.10.10 2.40.10.10
3lgy
R178a mutant of the degs-deltapdz protease
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (179 residues) CATH domains: 2.40.10.10 2.40.10.10
3lh1
Q191a mutant of the degs-deltapdz
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (199 residues) CATH domains: 2.40.10.10 2.40.10.10
3lh3
Dfp modified degs delta pdz
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3235, degs, hhob, htrh, jw3204. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, D, E, F, G, H, I (207 residues) CATH domains: 2.40.10.10 2.40.10.10
3mh4
Htra proteases are activated by a conserved mechanism that c triggered by distinct molecular cues
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (301 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10
3mh5
Htra proteases are activated by a conserved mechanism that c triggered by distinct molecular cues
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (191 residues) CATH domains: 2.40.10.10 2.40.10.10
3mh6
Htra proteases are activated by a conserved mechanism that c triggered by distinct molecular cues
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (392 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10 2.30.42.10
3mh7
Htra proteases are activated by a conserved mechanism that c triggered by distinct molecular cues
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degp. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (392 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10 2.30.42.10
3otp
Crystal structure of the degp dodecamer with a model substra
Source: Escherichia coli. Organism_taxid: 562. Gene: b0161, degp, htra, jw0157, ptd. Expressed in: escherichia coli. Expression_system_taxid: 562. Gallus gallus. Chicken. Organism_taxid: 9031. Gene: lysozymE C, lyz.
Chains: A, B, C, D, E, F (381 residues) CATH domains: Unassigned 2.30.42.10 2.30.42.10
3ou0
Re-refined 3cs0
Source: Escherichia coli. Organism_taxid: 562. Gene: b0161, degp, htra, jw0157, ptd. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Chain: A (392 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10 2.30.42.10
3sti
Crystal structure of the protease domain of degq from escher
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degq, hhoa, b3234, jw3203. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: B, C (184 residues) CATH domains: 2.40.10.10 2.40.10.10
3stj
Crystal structure of the protease + pdz1 domain of degq from escherichia coli
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: degq, hhoa, b3234, jw3203. Expressed in: escherichia coli. Expression_system_taxid: 562. Organism_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (300 residues) CATH domains: 2.40.10.10 2.40.10.10 2.30.42.10
4a8a
Asymmetric cryo-em reconstruction of e. Coli degq 12-mer in with lysozyme
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Tissue: periplasm. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gallus gallus. Chicken. Organism_taxid: 9031.
Chains: A, B, C, D, E, F, G, H, I, J, K, L (385 residues)
4a8b
Symmetrized cryo-em reconstruction of e. Coli degq 12-mer in with lysozymes
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Tissue: periplasm. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gallus gallus. Chicken. Organism_taxid: 9031.
Chains: A, B, C, D, E, F, G, H, I, J, K, L (384 residues)
4a8c
Symmetrized cryo-em reconstruction of e. Coli degq 12-mer in with a binding peptide
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D, E, F, G, H, I, J, K, L (395 residues)
4a8d
Degp dodecamer with bound omp
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562. Strain: clc198
Chains: A, B, C, D, E, F, G, H, I, J, K, L (384 residues)
4a9g
Symmetrized cryo-em reconstruction of e. Coli degq 24-mer in with beta-casein
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, Y (390 residues)