_EC 3.4.17.21 Glutamate carboxypeptidase Ii. 44 PDB entries  
EC 3.-.-.- Hydrolases. [21,435 PDB entries]
EC 3.4.-.- Acting on peptide bonds (peptide hydrolases). [6,954 PDB entries]
EC 3.4.17.- Metallocarboxypeptidases. [165 PDB entries]
EC 3.4.17.21 Glutamate carboxypeptidase Ii. [44 PDB entries]    
1z8l

Reaction: Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.
Other name(s): Folate hydrolase. N-acetylated-gamma-linked-acidic dipeptidase. Naaladase. Pteroylpoly-gamma-glutamate carboxypeptidase.
Cofactor(s): Zn(2+).
Comments: Hydrolyzes alpha-peptide bonds in Ac-Asp-Glu, Asp-Glu, and Glu-Glu, but also gamma-glutamyl bonds in gamma-Glu-Glu and folylpoly-gamma- glutamates. With folylpoly-gamma-glutamates, shows processive carboxypeptidase activity to produce pteroylmonoglutamate. Does not hydrolyze Ac-beta-Asp-Glu. Inhibited by quisqualic acid, Ac-beta-Asp-Glu, and 2-phosphonomethyl- pentanedioate. The release of C-terminal glutamate from folylpoly-gamma-glutamates is also catalyzed by Ec 3.4.17.11 and Ec 3.4.19.9.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 44 PDB entries in enzyme class E.C.3.4.17.21

  PDB code Protein
1z8l
Crystal structure of prostate-specific membrane antigen, a t marker and peptidase
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma.
Chains: A, B, C, D (695 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2c6c
Membrane-bound glutamate carboxypeptidase ii (gcpii) in complex with gpi-18431 (s)-2-(4- iodobenzylphosphonomethyl)-pentanedioic acid
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: brain. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's cells
Chain: A (682 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2c6g
Membrane-bound glutamate carboxypeptidase ii (gcpii) with bound glutamate
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: brain. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's cells
Chain: A (679 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2c6p
Membrane-bound glutamate carboxypeptidase ii (gcpii) in complex with phosphate anion
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: brain. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's cells
Chain: A (677 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2cij
Membrane-bound glutamate carboxypeptidase ii (gcpii) with bound methionine
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: brain. Tissue: nerve. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2 cells.
Chain: A (687 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2jbj
Membrane-bound glutamate carboxypeptidase ii (gcpii) in complex with 2-pmpa (2-phosphonomethyl-pentanedioic acid)
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: brain, prostate. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_variant: n. Expression_system_cell_line: schneider's cells
Chain: A (687 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2jbk
Membrane-bound glutamate carboxypeptidase ii (gcpii) in complex with quisqualic acid (quisqualate, alpha-amino-3,5- dioxo-1,2,4-oxadiazolidine-2-propanoic acid)
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: brain. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's cells
Chain: A (687 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2oot
A high resolution structure of ligand-free human glutamate carboxypeptidase ii
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (686 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2or4
A high resolution crystal structure of human glutamate carboxypeptidase ii in complex with quisqualic acid
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (689 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2pvv
Structure of human glutamate carboxypeptidase ii (gcpii) in with l-serine-o-sulfate
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2pvw
A high resolution structure of human glutamate carboxypeptid (gcpii) in complex with 2-(phosphonomethyl)pentanedioic aci
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (690 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2xef
Human glutamate carboxypeptidase ii in complex with antibody-recruiting molecule arm-p8
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: s2
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2xeg
Human glutamate carboxypeptidase ii in complex with antibody-recruiting molecule arm-p4
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: s2
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2xei
Human glutamate carboxypeptidase ii in complex with antibody-recruiting molecule arm-p2
Source: Homo sapiens. Human. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: s2
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
2xej
Human glutamate carboxypeptidase ii in complex with arm-m4, urea-based inhibitor
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: s2
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3bhx
X-ray structure of human glutamate carboxypeptidase ii (gcpi complex with a transition state analog of asp-glu
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3bi0
X-ray structure of human glutamate carboxypeptidase ii (gcpi complex with a transition state analog of glu-glu
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3bi1
X-ray structure of human glutamate carboxypeptidase ii (gcpi complex with a transition state analog of methotrexate-glu
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3bxm
Structure of an inactive mutant of human glutamate carboxype [gcpii(e424a)] in complex with n-acetyl-asp-glu (naag)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell: schneider's s2 cells. Synthetic: yes. Other_details: natural neurotransmitter in humans
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3d7d
A high resolution crystal structure of human glutamate carboxypeptidase ii (gcpii) in a complex with dcfbd, a urea inhibitor
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_cell_line: schneider's s2 cells.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3d7f
A high resolution crystal structure of human glutamate carboxypeptidase ii (gcpii) in a complex with dcit, a urea- inhibitor
Source: Homo sapiens. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_cell_line: schneider's s2 cells.
Chain: A (691 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3d7g
A high resolution crystal structure of human glutamate carboxypeptidase ii (gcpii) in a complex with dcmc, a urea- inhibitor
Source: Homo sapiens. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_cell_line: schneider's s2 cells.
Chain: A (691 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3d7h
A high resolution crystal structure of human glutamate carboxypeptidase ii (gcpii) in a complex with dcibzl, a ure inhibitor
Source: Homo sapiens. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_cell_line: schneider's s2 cells.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3fec
Crystal structure of human glutamate carboxypeptidase iii (gcpiii/naaladase ii), pseudo-unliganded
Source: Homo sapiens. Man. Organism_taxid: 9606. Gene: naalad2. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (689 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3fed
The high resolution structure of human glutamate carboxypept (gcpiii/naaladase ii) in complex with a transition state an glu-glu
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: naalad2. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (690 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3fee
The high resolution structure of human glutamate carboxypept (gcpiii/naaladase ii) in complex with quisqualic acid
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: naalad2. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (689 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3ff3
The high resolution structure of human glutamate carboxypept (gcpiii/naaladase ii) in complex with l-glutamate
Source: Homo sapiens. Man. Organism_taxid: 9606. Gene: naalad2. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (689 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3iww
Crystal structure of human glutamate carboxypeptidase ii (gc complex with dbibzl, a urea-based inhibitor
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2 cells.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3rbu
N-terminally avitev-tagged human glutamate carboxypeptidase complex with 2-pmpa
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider 2 (s2)
Chain: A (691 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3sje
X-ray structure of human glutamate carboxypeptidase ii (the inactive mutant) in complex with n-acetyl-aspartyl-aminonon
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma, gig27. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2 cells
Chain: A (690 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3sjf
X-ray structure of human glutamate carboxypeptidase ii in co a urea-based inhibitor (a25)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma, gig27. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2 cells
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3sjg
Human glutamate carboxypeptidase ii (e424a inactive mutant ) complex with n-acetyl-aspartyl-aminooctanoic acid
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2 cells
Chain: A (696 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
3sjx
X-ray structure of human glutamate carboxypeptidase ii (the inactive mutant) in complex with n-acetyl-aspartyl-methioni
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma, gig27. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2 cells
Chain: A (690 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4jyw
X-ray structure of human glutamate carboxypeptidase ii (gcpi complex with ctt1057
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell: s2.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4mcp
A high resolution structure of human glutamate carboxypeptid (gcpii) in complex with folyl-gamma-l-glutamic acid (pteroy l-glutamic acid)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma, gig27. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (693 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4mcq
A high resolution structure of human glutamate carboxypeptid (gcpii) in complex with folyldi-gamma-l-glutamic acid (pter gamma-l-glutamic acid)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma, gig27. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2
Chain: A (693 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4mcr
A high resolution structure of human glutamate carboxypeptid (gcpii) in complex with folyltri-gamma-l-glutamic acid (pte gamma-l-glutamic acid)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma, gig27. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2.
Chain: A (693 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4mcs
A high resolution structure of human glutamate carboxypeptid (gcpii) his475tyr variant in complex with glutamic acid
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma, gig27. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell: schneider's s2
Chain: A (691 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4oc0
X-ray structure of of human glutamate carboxypeptidase ii (g complex with ccibzl, a urea-based inhibitor n~2~-[(1- carboxycyclopropyl)carbamoyl]-n~6~-(4-iodobenzoyl)-l-lysine
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4oc1
X-ray structure of of human glutamate carboxypeptidase ii (g complex with coibzl, a urea-based inhibitor (2s)-2-[({(1s)- 2-[(2s)-oxiran-2-yl]ethyl}carbamoyl)amino]-6-[(4-iodobenzoy amino]hexanoic acid
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4oc2
X-ray structure of of human glutamate carboxypeptidase ii (g complex with ceibzl, a urea-based inhibitor n~2~-{[(1s)-1-c 3-yn-1-yl]carbamoyl}-n~6~-(4-iodobenzoyl)-l-lysine
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4oc3
X-ray structure of of human glutamate carboxypeptidase ii (g complex with cfibzl, a urea-based inhibitor n~2~-{[(1s)-1-c (furan-2-yl)ethyl]carbamoyl}-n~6~-(4-iodobenzoyl)-l-lysine
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4oc4
X-ray structure of of human glutamate carboxypeptidase ii (g complex with cpibzl, a urea-based inhibitor n~2~-{[(1s)-1-c (pyridin-4-yl)ethyl]carbamoyl}-n~6~-(4-iodobenzoyl)-l-lysin
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (690 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40
4oc5
X-ray structure of of human glutamate carboxypeptidase ii (g complex with chibzl, a urea-based inhibitor n~2~-{[(s)-carb hydroxyphenyl)methyl]carbamoyl}-n~6~-(4-iodobenzoyl)-l-lysi
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: folh, folh1, gig27, naalad1, psm, psma. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
Chain: A (694 residues) CATH domains: 3.40.630.10 3.50.30.30 1.20.930.40