_EC 3.2.1.45 Glucosylceramidase. 23 PDB entries  
EC 3.-.-.- Hydrolases. [20,839 PDB entries]
EC 3.2.-.- Glycosylases. [4,000 PDB entries]
EC 3.2.1.- Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compound [3,482 PDB entries]
EC 3.2.1.45 Glucosylceramidase. [23 PDB entries]    
1ogs

Reaction: D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine.
 


D-glucosyl-N-acylsphingosine
+ H(2)O
=
D-glucose
+
N-acylsphingosine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Acid beta-glucosidase. Beta-glucocerebrosidase. D-glucosyl-N-acylsphingosine glucohydrolase.
Comments: Also acts on glucosylsphingosine (cf. Ec 3.2.1.62).
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 23 PDB entries in enzyme class E.C.3.2.1.45

  PDB code Protein
1ogs
Human acid-beta-glucosidase
Source: Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: cho
Chains: A, B (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose
1y7v
X-ray structure of human acid-beta-glucosidase covalently bo conduritol b epoxide
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: ovary cells
Chains: A, B (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group INS is 91.67% similar to enzyme product D-glucose
  Het Group NAG is 40.74% similar to enzyme product N-acylsphingosine
2f61
Crystal structure of partially deglycosylated acid beta-gluc
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: cho. Expression_system_cell: ovary
Chains: A, B (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NDG is 62.50% similar to enzyme product D-glucose
2j25
Partially deglycosylated glucoceramidase
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: chinese hamster ovary cells
Chains: A, B (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group BMA is 91.00% similar to enzyme product D-glucose
  Het Group NAG is 40.00% similar to enzyme product N-acylsphingosine
2nsx
Structure of acid-beta-glucosidase with pharmacological chap provides insight into gaucher disease
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_organ: ovary
Chains: A, B, C, D (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NDG is 62.50% similar to enzyme product D-glucose
2nt0
Acid-beta-glucosidase low ph, glycerol bound
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_organ: ovary
Chains: A, B, C, D (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose
2nt1
Structure of acid-beta-glucosidase at neutral ph
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_organ: ovary
Chains: A, B, C, D (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose
2v3d
Acid-beta-glucosidase with n-butyl-deoxynojirimycin
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: daucus carota. Expression_system_taxid: 4039
Chains: A, B (496 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group BMA is 91.00% similar to enzyme product D-glucose
  Het Group NAG is 40.00% similar to enzyme product N-acylsphingosine
2v3e
Acid-beta-glucosidase with n-nonyl-deoxynojirimycin
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: daucus carota. Expression_system_taxid: 4039
Chains: A, B (498 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group BMA is 91.00% similar to enzyme product D-glucose
  Het Group NAG is 40.00% similar to enzyme product N-acylsphingosine
2v3f
Acid-beta-glucosidase produced in carrot
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: daucus carota. Expression_system_taxid: 4039
Chains: A, B (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group MAN is 91.00% similar to enzyme product D-glucose
  Het Group NAG is 40.00% similar to enzyme product N-acylsphingosine
2vt0
X-ray structure of a conjugate with conduritol-beta-epoxide of acid-beta-glucosidase overexpressed in cultured plant cells
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: lysosome. Expressed in: daucus carota. Expression_system_taxid: 4039
Chains: A, B (495 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group CBU is 91.67% similar to enzyme product D-glucose
  Het Group NAG is 40.74% similar to enzyme product N-acylsphingosine
2wcg
X-ray structure of acid-beta-glucosidase with n-octyl( cyclic guanidine)-nojirimycin in the active site
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: daucus carota. Expression_system_taxid: 4039
Chains: A, B (493 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group MAN is 91.00% similar to enzyme product D-glucose
  Het Group NAG is 40.00% similar to enzyme product N-acylsphingosine
2wkl
Velaglucerase alfa
Source: Homo sapiens. Human. Organism_taxid: 9606
Chains: A, B (495 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.00% similar to enzyme product D-glucose
2xwd
X-ray structure of acid-beta-glucosidase with 5n,6o-(n'-(n- octyl)imino)nojirimycin in the active site
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: daucus carota. Expression_system_taxid: 4039
Chains: A, B (493 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group MAN is 91.67% similar to enzyme product D-glucose
  Het Group NAG is 40.74% similar to enzyme product N-acylsphingosine
2xwe
X-ray structure of acid-beta-glucosidase with 5n,6s-(n'-(n- octyl)imino)-6-thionojirimycin in the active site
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: daucus carota. Expression_system_taxid: 4039
Chains: A, B (496 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligands:   Het Group BMA is 91.67% similar to enzyme product D-glucose
  Het Group NAG is 40.74% similar to enzyme product N-acylsphingosine
3gxd
Crystal structure of apo acid-beta-glucosidase ph 4.5
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: cricetulus griseus.
Chains: A, B, C, D (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose
3gxf
Crystal structure of acid-beta-glucosidase with isofagomine ph
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: cricetulus griseus.
Chains: A, B, C, D (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose
3gxi
Crystal structure of acid-beta-glucosidase at ph 5.5
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: cricetulus griseus. Expression_system_taxid: 10029
Chains: A, B, C, D (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose
3gxm
Crystal structure of acid-beta-glucosidase at ph 4.5, phosph crystallization condition
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: cricetulus griseus. Expression_system_taxid: 10029
Chains: A, B, C, D (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose
3ke0
Crystal structure of n370s glucocerebrosidase at acidic ph.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108
Chains: A, B (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NDG is 62.00% similar to enzyme product D-glucose
3keh
Crystal structure of n370s glucocerebrosidase mutant at ph 7
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108
Chains: A, B (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NDG is 62.00% similar to enzyme product D-glucose
3rik
The acid beta-glucosidase active site exhibits plasticity in 3,4,5,6-tetrahydroxyazepane-based inhibitors: implications pharmacological chaperone design for gaucher disease
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: cricetulus griseus. Expression_system_taxid: 10029
Chains: A, B, C, D (497 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose
3ril
The acid beta-glucosidase active site exhibits plasticity in 3,4,5,6-tetrahydroxyazepane-based inhibitors: implications pharmacological chaperone design for gaucher disease
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: cricetulus griseus. Expression_system_taxid: 10029
Chains: A, B, C, D (496 residues) CATH domains: 3.20.20.80 2.60.40.1180
Bound ligand:   Het Group NAG is 62.50% similar to enzyme product D-glucose