_EC 3.2.1.179 Gellan tetrasaccharide unsaturated glucuronyl hydrolase. 7 PDB entries  
EC 3.-.-.- Hydrolases. [21,295 PDB entries]
EC 3.2.-.- Glycosylases. [4,193 PDB entries]
EC 3.2.1.- Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compound [3,658 PDB entries]
EC 3.2.1.179 Gellan tetrasaccharide unsaturated glucuronyl hydrolase. [7 PDB entries]    
1vd5

Reaction: Beta-D-4-deoxy-Delta(4)-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap- (1->3)-beta-D-Glcp + H(2)O = 5-dehydro-4-deoxy-D-glucuronate + beta-D- Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp.
 

Beta-D-4-deoxy-Delta(4)-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap- (1->3)-beta-D-Glcp
+ H(2)O
=
5-dehydro-4-deoxy-D-glucuronate
+ beta-D- Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Unsaturated glucuronyl hydrolase.
Comments: The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases, e.g. the tetrasaccharide beta-D-4-deoxy-Delta(4)-GlcAp-(1->4)-beta-D-Glcp- (1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp produced by Ec 4.2.2.25. The enzyme can also hydrolyze unsaturated chondroitin and hyaluronate disaccharides (beta-D-4-deoxy-Delta(4)-GlcAp-(1->3)-beta-D-GalNAc, beta-D-4-deoxy-Delta(4)-GlcAp-(1->3)-beta-D-GalNAc6S, beta-D-4-deoxy- Delta(4)-GlcAp2S-(1->3)-beta-D-GalNAc, beta-D-4-deoxy-Delta(4)-GlcAp- (1->3)-beta-D-GlcNAc), preferring the unsulfated disaccharides to the sulfated disaccharides.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 7 PDB entries in enzyme class E.C.3.2.1.179

  PDB code Protein
1vd5
Crystal structure of unsaturated glucuronyl hydrolase, respo the degradation of glycosaminoglycan, from bacillus sp. Gl1 resolution
Source: Bacillus sp.. Organism_taxid: 84635. Strain: gl1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (377 residues) CATH domain: 1.50.10.10
2ahf
Unsaturated glucuronyl hydrolase mutant d88n
Source: Bacillus sp.. Organism_taxid: 84635. Strain: gl1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (377 residues) CATH domain: 1.50.10.10
2ahg
Unsaturated glucuronyl hydrolase mutant d88n with dglca-galn
Source: Bacillus sp.. Organism_taxid: 84635. Strain: gl1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (377 residues) CATH domain: 1.50.10.10
2d5j
Unsaturated glucuronyl hydrolase triggers hydration of vinyl group but not of glycosidic bond
Source: Bacillus sp.. Organism_taxid: 84635. Strain: gl1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (377 residues) CATH domain: 1.50.10.10
2fuz
Ugl hexagonal crystal structure without glycine and dtt mole
Source: Bacillus sp.. Organism_taxid: 84635. Strain: gl1. Gene: ugl. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (377 residues) CATH domain: 1.50.10.10
2fv0
Ugl_d88n/dglca-glc-rha-glc
Source: Bacillus sp.. Organism_taxid: 84635. Strain: gl1. Gene: ugl. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (377 residues) CATH domain: 1.50.10.10
2fv1
Ugl_d88n/dglca-glcnac
Source: Bacillus sp.. Organism_taxid: 84635. Strain: gl1. Gene: ugl. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (377 residues) CATH domain: 1.50.10.10