_EC 3.1.26.3 Ribonuclease Iii. 38 PDB entries  
EC 3.-.-.- Hydrolases. [21,051 PDB entries]
EC 3.1.-.- Acting on ester bonds. [5,973 PDB entries]
EC 3.1.26.- Endoribonucleases producing 5'-phosphomonoesters. [1,215 PDB entries]
EC 3.1.26.3 Ribonuclease Iii. [38 PDB entries]    
1i4s

Reaction: Endonucleolytic cleavage to 5'-phosphomonoester.
Other name(s): Ribonuclease 3. RNase Iii.
Comments: An endoribonuclease that cleaves double-stranded Rna molecules. The cleavage can be either a single-stranded nick or double-stranded break in the Rna, depending in part upon the degree of base-pairing in the region of the cleavage site. Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage. RNase Iii is involved in both rRNA processing and mRNA processing and decay.
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There are 38 PDB entries in enzyme class E.C.3.1.26.3

  PDB code Protein
1i4s
Crystal structure of rnase iii endonuclease domain from aquifex aeolicus at 2.15 angstrom resolution
Source: Aquifex aeolicus. Organism_taxid: 63363. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (147 residues) CATH domain: 1.10.1520.10
1jfz
Crystal structure of mn(ii)-complex of rnase iii endonuclease domain from aquifex aeolicus at 2.10 angstrom resolution
Source: Aquifex aeolicus. Organism_taxid: 63363. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B, C, D (148 residues) CATH domain: 1.10.1520.10
1o0w
Crystal structure of ribonuclease iii (tm1102) from thermotoga maritima at 2.0 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm1102. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (238 residues) CATH domains: 1.10.1520.10 3.30.160.20
1rc5
Crystal structure of mg(ii)-complex of rnase iii endonuclease domain from aquifex aeolicus at 2.30 angstrom resolution
Source: Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc, aq_946. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B, C, D (148 residues) CATH domain: 1.10.1520.10
1rc7
Crystal structure of rnase iii mutant e110k from aquifex aeolicus complexed with ds-RNA at 2.15 angstrom resolution
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc, aq_946. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (220 residues) CATH domains: 1.10.1520.10 3.30.160.20
1t4l
Solution structure of double-stranded RNA binding domain of s. Cerevisiae rnase iii (rnt1p) in complex with the 5' terminal RNA hairpin of snr47 precursor
Source: Synthetic: yes. Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: rnt1, ymr239c, ym9408.01c, ym9959.21. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: B (90 residues) CATH domain: 3.30.160.20
1t4n
Solution structure of rnt1p dsrbd
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: rnt1, ymr239c, ym9408.01c, ym9959.21. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (88 residues) CATH domain: 3.30.160.20
1t4o
Crystal structure of rnt1p dsrbd
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: rnt1, ymr239c, ym9408.01c, ym9959.21. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (81 residues) CATH domain: 3.30.160.20
1yyk
Crystal structure of rnase iii from aquifex aeolicus complexed with double-stranded RNA at 2.5-angstrom resolution
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (221 residues) CATH domains: 1.10.1520.10 3.30.160.20
1yyo
Crystal structure of rnase iii mutant e110k from aquifex aeolicus complexed with double-stranded RNA at 2.9- angstrom resolution
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (220 residues) CATH domains: 1.10.1520.10 3.30.160.20
1yyw
Crystal structure of rnase iii from aquifex aeolicus complexed with double stranded RNA at 2.8-angstrom resolution
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (219 residues) CATH domains: 1.10.1520.10 3.30.160.20
1yz9
Crystal structure of rnase iii mutant e110q from aquifex aeolicus complexed with double stranded RNA at 2.1- angstrom resolution
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (220 residues) CATH domains: 1.10.1520.10 3.30.160.20
2a11
Crystal structure of nuclease domain of ribonuclase iii from mycobacterium tuberculosis
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (154 residues) CATH domain: 1.10.1520.10
2eb1
Crystal structure of thE C-terminal rnase iii domain of huma
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (171 residues) CATH domain: 1.10.1520.10
2ez6
Crystal structure of aquifex aeolicus rnase iii (d44n) complexed with product of double-stranded RNA processing
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (218 residues) CATH domains: 1.10.1520.10 3.30.160.20
2khx
Drosha double-stranded RNA binding motif
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: rnasen, rn3, rnase3l. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (79 residues) CATH domain: 3.30.160.20
2lbs
Solution structure of double-stranded RNA binding domain of cerevisiae rnase iii (rnt1p) in complex with aagu tetraloop
Source: Synthetic: yes. Saccharomyces cerevisiae. Yeast. Organism_taxid: 4932. Gene: rnt1, ym9408.01c, ym9959.21, ymr239c. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: B (90 residues) CATH domain: 3.30.160.20
2lup
Rdc refined solution structure of double-stranded RNA bindin of s. Cerevisiae rnase iii (rnt1p) in complex with the term hairpin of snr47 precursor
Source: Synthetic: yes. Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Variant: ymr239c. Gene: rnt1, ym9408.01c, ym9959.21, ymr239c. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: B (90 residues) CATH domain: 3.30.160.20
2luq
Solution structure of double-stranded RNA binding domain of s.Cerevisiae rnase iii (rnt1p)
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Variant: ymr239c. Gene: rnt1, ym9408.01c, ym9959.21, ymr239c. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (90 residues) CATH domain: 3.30.160.20
2nue
Crystal structure of rnase iii from aquifex aeolicus complexed with ds-RNA at 2.9-angstrom resolution
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (220 residues) CATH domains: 1.10.1520.10 3.30.160.20
2nuf
Crystal structure of rnase iii from aquifex aeolicus complexed with ds-RNA at 2.5-angstrom resolution
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (219 residues) CATH domains: 1.10.1520.10 3.30.160.20
2nug
Crystal structure of rnase iii from aquifex aeolicus complexed with ds-RNA at 1.7-angstrom resolution
Source: Synthetic: yes. Aquifex aeolicus. Organism_taxid: 63363. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (216 residues) CATH domains: 1.10.1520.10 3.30.160.20
3c4b
Structure of rnaseiiib and dsrna binding domains of mouse di
Source: Mus musculus. House mouse. Organism_taxid: 10090. Strain: mouse. Gene: dicer1, dicer, mdcr. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (245 residues) CATH domain: 1.10.1520.10
3c4t
Structure of rnaseiiib and dsrna binding domains of mouse di
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: dicer1, dicer, mdcr. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (242 residues) CATH domain: 1.10.1520.10
3n3w
2.2 angstrom resolution crystal structure of nuclease domain ribonuclase iii (rnc) from campylobacter jejuni
Source: Campylobacter jejuni subsp. Jejuni. Organism_taxid: 192222. Strain: nctc 11168. Gene: rnc. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (144 residues) CATH domain: 1.10.1520.10
3o2r
Structural flexibility in region involved in dimer formation nuclease domain of ribonuclase iii (rnc) from campylobacter
Source: Campylobacter jejuni subsp. Jejuni. Organism_taxid: 192222. Strain: nctc 11168. Gene: cjsa_1547, rnc. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D (144 residues) CATH domain: 1.10.1520.10
4m2z
Crystal structure of rnase iii complexed with double-strande cmp (type ii cleavage)
Source: Aquifex aeolicus. Organism_taxid: 224324. Strain: vf5. Gene: aq_946, rnc. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: cleavage product of a r1.1 RNA derivative
Chains: A, B (220 residues) CATH domains: 1.10.1520.10 3.30.160.20
4m30
Crystal structure of rnase iii complexed with double-strande amp (type ii cleavage)
Source: Aquifex aeolicus. Organism_taxid: 224324. Strain: vf5. Gene: aq_946, rnc. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: cleavage product of a r1.1 RNA derivative
Chains: A, B (220 residues) CATH domains: 1.10.1520.10 3.30.160.20
4ngb
Structure of human dicer platform-paz-connector helix casset complex with 12-mer sirna having uu-3' ends (2.25 angstrom resolution)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chain: A (276 residues)
4ngc
Structure of human dicer platform-paz-connector helix casset complex with 12-mer sirna having ua-3' ends (2.1 angstrom r
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chain: A (276 residues)
4ngd
Structure of human dicer platform-paz-connector helix casset complex with 12-mer sirna having 5'-p and uu-3' ends (1.95 resolution)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chain: A (280 residues)
4ngf
Structure of human dicer platform-paz-connector helix casset complex with 17-mer sirna having 5'-p and uu-3' ends (3.1 a resolution)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chains: A, B, C, D (260 residues)
4ngg
Structure of human dicer platform-paz-connector helix casset complex with 13-mer sirna having 5'-a and uu-3' ends (2.6 a resolution)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chain: A (267 residues)
4nh3
Structure of human dicer platform-paz-connector helix casset complex with 13-mer sirna having 5'-pu and uu-3' ends (2.6 resolution)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chain: A (268 residues)
4nh5
Structure of human dicer platform-paz-connector helix casset complex with 14-mer sirna having 5'-puu and uu-3' ends (2.5 resolution)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chain: A (271 residues)
4nh6
Structure of human dicer platform-paz-connector helix casset complex with 15-mer sirna having 5'-puuu and uu-3' ends (2. Angstrom resolution)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chain: A (272 residues)
4nha
Structure of human dicer platform-paz-connector helix casset complex with 16-mer sirna having 5'-p and uu-3' ends (3.4 a resolution)
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: dicer, dicer1, herna, kiaa0928. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Chain: A (234 residues)
4oog
Crystal structure of yeast rnase iii (rnt1p) complexed with product of dsrna processing
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: rnt1, ym9408.01c, ym9959.21, ymr239c. Expressed in: escherichia coli. Expression_system_taxid: 1007065. Synthetic: yes. Other_details: derived from u5 snrna 3' end cleavage produc
Chains: A, B, C (109 residues)