_EC 3.1.21.4 Type Ii site-specific deoxyribonuclease. 100 PDB entries  
EC 3.-.-.- Hydrolases. [21,295 PDB entries]
EC 3.1.-.- Acting on ester bonds. [6,030 PDB entries]
EC 3.1.21.- Endodeoxyribonucleases producing 5'-phosphomonoesters. [141 PDB entries]
EC 3.1.21.4 Type Ii site-specific deoxyribonuclease. [100 PDB entries]    
1az0

Reaction: Endonucleolytic cleavage of Dna to give specific double-stranded fragments with terminal 5'-phosphates.
Other name(s): Type Ii restriction enzyme.
Cofactor(s): Mg(2+).
Comments: Large group of enzymes which recognize specific short Dna sequences and cleave either within, or at a short specific distance from, the recognition site. See the Rebase database for a complete list of these enzymes: http://rebase.neb.com/rebase/
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 100 PDB entries in enzyme class E.C.3.1.21.4

  PDB code Protein
1az0
Ecorv endonuclease/DNA complex
Source: Escherichia coli. Organism_taxid: 562
Chains: A, B (237 residues) CATH domain: 3.40.600.10
1az3
Ecorv endonuclease, unliganded, form b
Source: Escherichia coli. Organism_taxid: 562
Chains: A, B (186 residues) CATH domain: 3.40.600.10
1az4
Ecorv endonuclease, unliganded, form b, t93a mutant
Source: Escherichia coli. Organism_taxid: 562
Chains: A, B (187 residues) CATH domain: 3.40.600.10
1b94
Restriction endonuclease ecorv with calcium
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Cellular_location: cytoplasm. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1b95
Analysis of a mutational hot-spot in the ecorv restriction endonuclease: a catalytic role for a main chain carbonyl group
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Cellular_location: cytoplasm. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1b96
Analysis of a mutational hot-spot in the ecorv restriction endonuclease: a catalytic role for a main chain carbonyl group
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Cellular_location: cytoplasm. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1b97
Analysis of a mutational hot-spot in the ecorv restriction endonuclease: a catalytic role for a main chain carbonyl group
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Cellular_location: cytoplasm. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1bam
Structure of restriction endonuclease bamhi phased at 1.95 angstroms resolution by mad analysis
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (200 residues) CATH domain: 3.40.91.20
1bgb
Ecorv endonuclease complex with 5'-cgggatatccc DNA
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (237 residues) CATH domain: 3.40.600.10
1bhm
Restriction endonuclease bamhi complex with DNA
Source: Synthetic: yes. Bacillus amyloliquefaciens. Organism_taxid: 1390
Chains: A, B (198 residues) CATH domain: 3.40.91.20
1bss
Ecorv-t93a/DNA/ca2+
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (230 residues) CATH domain: 3.40.600.10
1bsu
Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (236 residues) CATH domain: 3.40.600.10
1bua
Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (231 residues) CATH domain: 3.40.600.10
1cfr
Crystal structure of citrobacter freundii restriction endonu cfr10i at 2.15 angstroms resolution.
Source: Citrobacter freundii. Organism_taxid: 546. Strain: rfl10i. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (283 residues) CATH domain: 3.40.91.10
1ckq
Pre-transition state eco ri endonuclease/cognate DNA (tcgcgaattcgcg) complex
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562
Chain: A (261 residues) CATH domain: 3.40.580.10
1cl8
A pre-transition state eco ri endonuclease/cognate DNA (tcgcgapttcgcg) complex with DNA base analog purine (p)
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562
Chain: A (261 residues) CATH domain: 3.40.580.10
1d02
Crystal structure of muni restriction endonuclease in comple cognate DNA
Source: Synthetic: yes. Other_details: decameric oligonucleotide 5'-ds(gpcpcpapaptp was synthesized with standard procedures. Mycoplasma. Organism_taxid: 2093. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (197 residues) CATH domain: 3.40.580.10
1d2i
Crystal structure of restriction endonuclease bglii complexe 16-mer
Source: Synthetic: yes. Other_details: oligomers were produced commercially. Bacillus subtilis. Organism_taxid: 1423
Chains: A, B (223 residues) CATH domain: 3.40.91.20
1dc1
Restriction enzyme bsobi/DNA complex structure: encirclement of the DNA and histidine-catalyzed hydrolysis within a canonical restriction enzyme fold
Source: Synthetic: yes. Other_details: DNA sequence designed for crystallization based on the central recognition sequence of bacillus stearothermophilus. Geobacillus stearothermophilus. Organism_taxid: 1422. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (310 residues) CATH domains: 3.40.91.10 1.10.238.90
1dfm
Crystal structure of restriction endonuclease bglii complexed with DNA 16-mer
Source: Synthetic: yes. Bacillus subtilis. Organism_taxid: 1423. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (223 residues) CATH domain: 3.40.91.20
1dmu
Crystal structure of the restriction endonuclease bgli (E.C.3.1.21.4) bound to its DNA recognition sequence
Source: Synthetic: yes. Other_details: contains DNA recognition sequence of bgli. Bacillus subtilis. Organism_taxid: 1423. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (299 residues) CATH domain: 3.40.600.20
1eo3
Inhibition of ecorv endonuclease by deoxyribo-3'-s- phosphorothiolates: a high resolution x-ray crystallographic study
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (241 residues) CATH domain: 3.40.600.10
1eo4
Ecorv bound to mn2+ and cognate DNA containing a 3's substition at the cleavage site
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (238 residues) CATH domain: 3.40.600.10
1eon
Ecorv bound to 3'-s-phosphorothiolate DNA and ca2+
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (236 residues) CATH domain: 3.40.600.10
1eoo
Ecorv bound to cognate DNA
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1eop
Ecorv bound to cognate DNA
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (238 residues) CATH domain: 3.40.600.10
1eri
X-ray structure of the DNA-eco ri endonuclease-DNA recognition complex: the recognition network and the integration of recognition and cleavage
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562
Chain: A (261 residues) CATH domain: 3.40.580.10
1es8
Crystal structure of free bglii
Source: Bacillus subtilis. Organism_taxid: 1423. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (197 residues) CATH domain: 3.40.91.20
1esg
Restriction endonuclease bamhi bound to a non-specific DNA.
Source: Synthetic: yes. Other_details: phosphoramite synthesis. Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (209 residues) CATH domain: 3.40.91.20
1ev7
Crystal structure of DNA restriction endonuclease naei
Source: Lechevalieria aerocolonigenes. Organism_taxid: 68170. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (295 residues) CATH domains: 3.40.600.10 1.10.10.10
1eyu
High resolution structure of the pvuii endonculease/cognate DNA complex at ph 4.6
Source: Synthetic: yes. Proteus vulgaris. Organism_taxid: 585. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (156 residues) CATH domain: 3.40.210.10
1f0o
Pvuii endonuclease/cognate DNA complex (glutaraldehyde- crosslinked crystal) at ph 7.5 with two calcium ions at each active site
Source: Synthetic: yes. Proteus vulgaris. Organism_taxid: 585. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (154 residues) CATH domain: 3.40.210.10
1fiu
Tetrameric restriction endonuclease ngomiv in complex with cleaved DNA
Source: Synthetic: yes. Neisseria gonorrhoeae. Organism_taxid: 485. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (286 residues) CATH domain: 3.40.50.10010
1fok
Structure of restriction endonuclease foki bound to DNA
Source: Planomicrobium okeanokoites. Organism_taxid: 244. Strain: ifo12536. Atcc: 33414. Gene: foki. Expressed in: escherichia coli. Expression_system_taxid: 562. Organism_taxid: 244
Chain: A (568 residues) CATH domains: 3.90.241.10 1.10.10.10 3.40.91.30
1h56
Structural and biochemical characterization of a new magnesium ion binding site near tyr94 in the restriction endonuclease pvuii
Source: Proteus vulgaris. Organism_taxid: 585. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (156 residues) CATH domain: 3.40.210.10
1iaw
Crystal structure of naei complexed with 17mer DNA
Source: Synthetic: yes. Lechevalieria aerocolonigenes. Organism_taxid: 68170. Gene: nae i. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (304 residues) CATH domains: 3.40.600.10 1.10.10.10
1k0z
Crystal structure of the pvuii endonuclease with pr3+ and so4 ions bound in the active site at 2.05a.
Source: Proteus vulgaris. Organism_taxid: 585. Gene: pvuiir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (151 residues) CATH domain: 3.40.210.10
1kc6
Hincii bound to cognate DNA
Source: Synthetic: yes. Haemophilus influenzae. Organism_taxid: 727. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D (249 residues) CATH domain: 3.40.600.10
1na6
Crystal structure of restriction endonuclease ecorii mutant r88a
Source: Escherichia coli. Organism_taxid: 562. Gene: ecorii. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (365 residues) CATH domain: 2.40.330.10
1ni0
Structure of the y94f mutant of the restriction endonuclease pvuii
Source: Proteus vulgaris. Organism_taxid: 585. Gene: pvuiir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (157 residues) CATH domain: 3.40.210.10
1pvi
Structure of pvuii endonuclease with cognate DNA
Source: Synthetic: yes. Proteus vulgaris. Organism_taxid: 585
Chains: A, B (156 residues) CATH domain: 3.40.210.10
1pvu
The crystal structure of pvuii endonuclease reveals extensive structural homologies to ecorv
Source: Proteus vulgaris. Organism_taxid: 585
Chains: A, B (154 residues) CATH domain: 3.40.210.10
1qc9
The crystallographic structure of restriction endonuclease e 3.3 a in the absense of DNA
Source: Escherichia coli. Organism_taxid: 562
Chains: A, B, C (253 residues) CATH domain: 3.40.580.10
1qps
The crystal structure of a post-reactive cognate DNA-eco ri complex at 2.50 a in the presence of mn2+ ion
Source: Synthetic: yes. Other_details: recognition sequence. Escherichia coli. Organism_taxid: 562
Chain: A (261 residues) CATH domain: 3.40.580.10
1qrh
X-ray structure of the DNA-eco ri endonuclease complexes with an r145k mutation at 2.7 a
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562
Chain: A (261 residues) CATH domain: 3.40.580.10
1qri
X-ray structure of the DNA-eco ri endonuclease complexes with an e144d mutation at 2.7 a
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562
Chain: A (261 residues) CATH domain: 3.40.580.10
1rv5
Complex of ecorv endonuclease with d(aaagat)/d(atctt)
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562
Chains: A, B (240 residues) CATH domain: 3.40.600.10
1rva
Mg2+ binding to the active site of eco rv endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 angstroms resolution
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1rvb
Mg2+ binding to the active site of eco rv endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 angstroms resolution
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1rvc
Mg2+ binding to the active site of eco rv endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 angstroms resolution
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1rve
The crystal structure of ecorv endonuclease and of its complexes with cognate and non-cognate DNA fragments
Source: Escherichia coli. Organism_taxid: 562
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1sa3
An asymmetric complex of restriction endonuclease mspi on its palindromic DNA recognition site
Source: Synthetic: yes. Moraxella sp.. Organism_taxid: 479. Gene: mspir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (262 residues) CATH domain: Unassigned
1stx
Structure of the k38a mutant of ecorv bound to cognate DNA and mn2+
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1suz
The structure of k92a ecorv bound to cognate DNA and mg2+
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (243 residues) CATH domain: 3.40.600.10
1sx5
K38a ecorv bound to cleaved DNA and mn2+: p1 crystal form
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562. Mk+).
Chains: A, B (244 residues) CATH domain: 3.40.600.10
1sx8
Ecorv bound to cognate DNA and mn2+
Source: Synthetic: yes. Other_details: synthetic. Escherichia coli. Organism_taxid: 562. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562. Mk+).
Chains: A, B (241 residues) CATH domain: 3.40.600.10
1tw8
Hincii bound to ca2+ and cognate DNA gtcgac
Source: Synthetic: yes. Other_details: commercial phosphoramidite chemistry. Haemophilus influenzae. Organism_taxid: 727. Gene: hinc ii. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D (257 residues) CATH domain: 3.40.600.10
1tx3
Hincii bound to cognate DNA
Source: Synthetic: yes. Haemophilus influenzae. Organism_taxid: 727. Gene: hindiir, hi0512. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D (249 residues) CATH domain: 3.40.600.10
1xhu
Hincii bound to cleaved, cognate DNA containing gtcgac
Source: Synthetic: yes. Other_details: phosphoramidite synthetic chemistry. Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D (257 residues) CATH domain: 3.40.600.10
1xhv
Hincii bound to cleaved cognate DNA gtcgac and mn2+
Source: Synthetic: yes. Other_details: phosphoramidite synthetic chemistry. Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D (254 residues) CATH domain: 3.40.600.10
1yfi
Crystal structure of restriction endonuclease mspi in complex with its cognate DNA in p212121 space group
Source: Synthetic: yes. Moraxella sp.. Organism_taxid: 479. Gene: mspir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (262 residues) CATH domain: Unassigned
1yuv
Crystal structure of the restriction endonuclease bstyi complex with DNA
Source: Geobacillus stearothermophilus. Bacteria. Gene: bstyir. Expressed in: escherichia coli. Synthetic: yes
Chains: A, B (203 residues) CATH domain: 3.40.91.20
2aud
Unliganded hincii
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (242 residues) CATH domain: 3.40.600.10
2b0d
Ecorv restriction endonuclease/gaattc/ca2+
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (236 residues) CATH domain: 3.40.600.10
2b0e
Ecorv restriction endonuclease/gaautc/ca2+
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (233 residues) CATH domain: 3.40.600.10
2bam
Restriction endonuclease bamhi complex with DNA and calcium reactive complex).
Source: Synthetic: yes. Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (207 residues) CATH domain: 3.40.91.20
2e52
Crystal structural analysis of hindiii restriction endonucle complex with cognate DNA at 2.0 angstrom resolution
Source: Haemophilus influenzae. Organism_taxid: 71421. Strain: rd kw20. Gene: hindiiir. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes
Chains: A, C, B, D (299 residues) CATH domains: Unassigned Unassigned
2ezv
Crystal structure of tetrameric restriction endonuclease sfii bound to cognate DNA.
Source: Synthetic: yes. Other_details: e. Coli
Chains: A, B (267 residues) CATH domains: Unassigned Unassigned
2f03
Crystal structure of tetrameric restriction endonuclease sfii in complex with cognate DNA (partial bound form)
Source: Synthetic: yes. Other_details: e.Coli
Chains: A, C (269 residues) CATH domains: Unassigned Unassigned
2fok
Structure of restriction endonuclease foki
Source: Planomicrobium okeanokoites. Organism_taxid: 244. Strain: ifo12536. Atcc: atcc 33414. Collection: atcc 33414. Gene: foki. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (558 residues) CATH domains: 3.90.241.10 1.10.10.10 3.40.91.30
2ge5
Ecorv restriction endonucleasE C-terminal deletion mutant/gatatc/ca2+
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: ecorvr. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (219 residues) CATH domain: 3.40.600.10
2gie
Hincii bound to cognate DNA gttaac
Source: Synthetic: yes. Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (257 residues) CATH domain: 3.40.600.10
2gig
Alteration of sequence specificity of the type ii restrictio endonuclease hincii through an indirect readout mechanism
Source: Synthetic: yes. Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (249 residues) CATH domain: 3.40.600.10
2gih
Q138f hincii bound to cognate DNA gtcgac and ca2+
Source: Synthetic: yes. Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (257 residues) CATH domain: 3.40.600.10
2gii
Q138f hincii bound to cognate DNA gttaac
Source: Synthetic: yes. Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (238 residues) CATH domain: 3.40.600.10
2gij
Q138f hincii bound to cognate DNA gttaac and ca2+
Source: Synthetic: yes. Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (245 residues) CATH domain: 3.40.600.10
2oxv
Structure of the a138t promiscuous mutant of the ecori restriction endonuclease bound to its cognate recognition site.
Source: Escherichia coli. Organism_taxid: 562. Gene: ecorir. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Other_details: abi3900 DNA synthesizer
Chain: A (261 residues) CATH domain: 3.40.580.10
2pvi
Pvuii endonuclease complexed to an iodinated cognate DNA
Source: Synthetic: yes. Proteus vulgaris. Organism_taxid: 585. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (156 residues) CATH domain: 3.40.210.10
2reu
Crystal structure of thE C-terminal of sau3ai fragment
Source: Staphylococcus aureus. Organism_taxid: 1280. Gene: sau3air. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (236 residues) CATH domains: Unassigned Unassigned
2rve
The crystal structure of ecorv endonuclease and of its complexes with cognate and non-cognate DNA segments
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (213 residues) CATH domain: 3.40.600.10
3a4k
Crystal structural analysis of hindiii restriction endonucle complex with cognate DNA and divalent cations at 2.17 angst resolution
Source: Haemophilus influenzae. Organism_taxid: 71421. Strain: rd kw20. Gene: hindiiir. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Other_details: this DNA sequence synthesized chemically con cognate hindiiir recognition sequence and elongated scaffol
Chains: A, C, B, D (297 residues) CATH domains: Unassigned Unassigned
3bam
Restriction endonuclease bamhi complex with DNA and manganese ions (post-reactive complex)
Source: Synthetic: yes. Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (206 residues) CATH domain: 3.40.91.20
3e3y
Q138f hincii bound to gttaac and cocrystallized with 5 mm ca2+
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hindiir, hi0512. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chains: A, B (242 residues) CATH domain: 3.40.600.10
3e40
Q138f hincii bound to gttaac and cocrystallized with 5 mm ca2+
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hindiir, hi0512. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chains: A, B (247 residues) CATH domain: 3.40.600.10
3e41
Q138f hincii bound to gtcgac and 5 mm ca2+
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hindiir, hi0512. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chains: A, B (246 residues) CATH domain: 3.40.600.10
3e42
Q138f hincii bound to gtcgac and ca2+ (cocrystallized)
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hindiir, hi0512. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chains: A, B (248 residues) CATH domain: 3.40.600.10
3e43
Q138f hincii bound to gttaac and cocrystallized with 2.5 mm mgcl2
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hindiir, hi0512. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chains: A, B (248 residues) CATH domain: 3.40.600.10
3e44
Q138f hincii bound to cleaved DNA (gtt | aac) and mn2+
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hindiir, hi0512. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Chains: A, B (247 residues) CATH domain: 3.40.600.10
3e45
Q138f hincii bound to noncognate DNA (gtgcac) and ca2+
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hindiir, hi0512. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chains: A, B (246 residues) CATH domain: 3.40.600.10
3ebc
Structure of n141a hincii with cognate DNA
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: hinciir. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Chains: A, B (241 residues) CATH domain: 3.40.600.10
3hqg
Crystal structure of restriction endonuclease ecorii catalyt terminal domain in complex with cognate DNA
Source: Escherichia coli. Organism_taxid: 562. Gene: ecorii, ecoriir. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Chain: A (222 residues)
3ksk
Crystal structure of single chain pvuii
Source: Proteus vulgaris. Organism_taxid: 585. Gene: pvuii, pvuiir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (313 residues) CATH domain: Unassigned
3mx1
The structure of giy-yig endonuclease r.Eco29ki
Source: Escherichia coli. Organism_taxid: 562. Gene: eco29kir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (208 residues) CATH domain: Unassigned
3mx4
DNA binding and cleavage by the giy-yig endonuclease r.Eco29 inactive variant e142q
Source: Escherichia coli. Organism_taxid: 562. Gene: eco29kir. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Chains: A, B, C, D, E, F, G, H (213 residues) CATH domain: Unassigned
3nic
DNA binding and cleavage by the giy-yig endonuclease r.Eco29 inactive variant y49f
Source: Escherichia coli. Organism_taxid: 562. Strain: 29k. Gene: eco29kir. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Chains: A, B, C, D, E, F, G, H (210 residues) CATH domain: Unassigned
3pvi
D34g mutant of pvuii endonuclease complexed with cognate DNA shows that asp34 is directly involved in DNA recognition and indirectly involved in catalysis
Source: Synthetic: yes. Proteus vulgaris. Organism_taxid: 585. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (156 residues) CATH domain: 3.40.210.10
4abt
Crystal structure of type iif restriction endonuclease ngomi cognate uncleaved DNA
Source: Neisseria gonorrhoeae. Organism_taxid: 485. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 485
Chains: A, B (286 residues) CATH domain: 3.40.50.10010
4esj
Restriction endonuclease dpni in complex with target DNA
Source: Streptococcus pneumoniae. Organism_taxid: 170187. Strain: tigr4. Gene: dpnc, spr1665. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 32630. Other_details: synthetic oligonucleotide
Chains: A, B (246 residues) CATH domains: Unassigned Unassigned
4kyw
Restriction endonuclease dpni in complex with two DNA molecu
Source: Streptococcus pneumoniae. Organism_taxid: 170187. Strain: tigr4. Gene: dpnc, spr1665, sp_1850. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic oligonucleotide
Chain: A (254 residues) CATH domains: Unassigned Unassigned
4rve
The crystal structure of ecorv endonuclease and of its complexes with cognate and non-cognate DNA segments
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (240 residues) CATH domain: 3.40.600.10