_EC 2.8.1.8 Lipoyl synthase. 2 PDB entries  
EC 2.-.-.- Transferases. [17,949 PDB entries]
EC 2.8.-.- Transferring sulfur-containing groups. [198 PDB entries]
EC 2.8.1.- Sulfurtransferases. [84 PDB entries]
EC 2.8.1.8 Lipoyl synthase. [2 PDB entries]    
4u0o

Reaction: Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl- L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.
 


Protein N(6)-(octanoyl)lysine
+ 2 × sulfur-(sulfur carrier)
+
2 × S-adenosyl- L-methionine
=
protein N(6)-(lipoyl)lysine
+ 2 × (sulfur carrier)
+
2 × L-methionine
+
2 × 5'-deoxyadenosine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Lipoate synthase.
Cofactor(s): Iron-sulfur.
 

Iron-sulfur
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
Comments: Member of the 'AdoMet radical' (radical SAM) family, all members of which produce the 5'-deoxyadenosin-5'-yl radical and methionine from AdoMet (i.e. S-adenosylmethionine, or S-(5'-deoxyadenosin- 5'-yl)methionine), by the addition of an electron from an iron-sulfur center. The radical is converted into 5'-deoxyadenosine when it abstracts a hydrogen atom from C-6 and C-8, leaving reactive radicals at these positions so that they can add sulfur, with inversion of configuration. Catalyzes the final step in the de-novo biosynthesis of the lipoyl
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 2 PDB entries in enzyme class E.C.2.8.1.8

  PDB code Protein
4u0o
Crystal structure of thermosynechococcus elongatus lipoyl sy complexed with mta and dtt
Source: Thermosynechococcus elongatus. Organism_taxid: 197221. Strain: bp-1. Gene: lipa2, tll0574. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008
Chain: B (273 residues)
Bound ligands:   Het Group DTT is 41.67% similar to enzyme product L-methionine
  Het Group MTA is 90.00% similar to enzyme product 5'-deoxyadenosine
4u0p
The crystal structure of lipoyl synthase in complex with s-a homocysteine
Source: Thermosynechococcus elongatus. Organism_taxid: 197221. Strain: bp-1. Gene: lipa2, tll0574. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: B (284 residues)
Bound ligand:   Het Group SAH corresponds to enzyme product L-methionine