_EC 2.7.7.60 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase. 32 PDB entries  
EC 2.-.-.- Transferases. [17,772 PDB entries]
EC 2.7.-.- Transferring phosphorous-containing groups. [10,822 PDB entries]
EC 2.7.7.- Nucleotidyltransferases. [4,459 PDB entries]
EC 2.7.7.60 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase. [32 PDB entries]    
1h3m

Reaction: Ctp + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.
 


CTP
+
2-C-methyl-D-erythritol 4-phosphate
=
diphosphate
+
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase. Mct. Mep cytidylyltransferase.
Cofactor(s): Manganese or magnesium.
Comments: Atp or utp can replace ctp, but both are less effective. Gtp and ttp are not substrates. Forms part of an alternative nonmevalonate pathway for terpenoid biosynthesis.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 32 PDB entries in enzyme class E.C.2.7.7.60

  PDB code Protein
1h3m
Structure of 4-diphosphocytidyl-2c-methyl-d-erythritol synthetase
Source: Escherichia coli. Organism_taxid: 469008. Strain: bl21(de3). Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (215 residues) CATH domain: 3.90.550.10
1i52
Crystal structure of 4-diphosphocytidyl-2-c-methylerythritol synthase (ygbp) involved in mevalonate independent isopreno biosynthesis
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: ispd. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (225 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group CTP corresponds to enzyme reactant CTP
1ini
Crystal structure of 4-diphosphocytidyl-2-c-methylerythritol synthetase (ygbp) involved in mevalonate independent isopre biosynthesis, complexed with cdp-me and mg2+
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: ispd. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (225 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group CDM corresponds to enzyme product 4-(cytidine
1inj
Crystal structure of the apo form of 4-diphosphocytidyl-2-c- methylerythritol (cdp-me) synthetase (ygbp) involved in mev independent isoprenoid biosynthesis
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: ispd. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (213 residues) CATH domain: 3.90.550.10
1vgt
Crystal structure of 4-diphosphocytidyl-2c-methyl-d-erythrit synthase
Source: Escherichia coli. Organism_taxid: 562. Gene: ispd, b2747. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (207 residues) CATH domain: 3.90.550.10
1vgu
Crystal structure of 4-diphosphocytidyl-2c-methyl-d- erythritol synthase
Source: Escherichia coli. Organism_taxid: 562. Gene: ispd, b2747. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (214 residues) CATH domain: 3.90.550.10
1vgw
Crystal structure of 4-diphosphocytidyl-2c-methyl-d-erythrit synthase
Source: Neisseria gonorrhoeae. Organism_taxid: 485. Gene: ygbp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D, E, F (212 residues) CATH domain: 3.90.550.10
1vgz
Crystal structure of 4-diphosphocytidyl-2c-methyl-d-erythrit synthase
Source: Neisseria gonorrhoeae. Organism_taxid: 485. Gene: ygbp. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (212 residues) CATH domain: 3.90.550.10
1vpa
Crystal structure of 2-c-methyl-d-erythritol 4-phosphate cytidylyltransferase (tm1393) from thermotoga maritima at 2 resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm1393, ispd. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (221 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group CTP corresponds to enzyme reactant CTP
1w55
Structure of the bifunctional ispdf from campylobacter jejuni
Source: Campylobacter jejuni. Organism_taxid: 197. Strain: jejuni ntct 11168. Atcc: 33560. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (369 residues) CATH domains: 3.90.550.10 3.30.1330.50
Bound ligands:   Het Group C is 72.00% similar to enzyme reactant CTP
  Het Group GPP is 47.00% similar to enzyme product diphosphate
1w57
Structure of the bifunctional ispdf from campylobacter jejuni containing zn
Source: Campylobacter jejuni. Organism_taxid: 197. Strain: jejuni ntct 11168. Atcc: 33560. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (368 residues) CATH domains: 3.90.550.10 3.30.1330.50
Bound ligands:   Het Group C5P is 72.00% similar to enzyme reactant CTP
  Het Group GPP is 47.00% similar to enzyme product diphosphate
1w77
2c-methyl-d-erythritol 4-phosphate cytidylyltransferase (ispd) from arabidopsis thaliana
Source: Arabidopsis thaliana. Mouse-ear cress. Organism_taxid: 3702. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (212 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group C5P is 72.00% similar to enzyme reactant CTP
2px7
Crystal structure of 2-c-methyl-d-erythritol 4-phosphate cytidylyltransferase from thermus thermophilus hb8
Source: Thermus thermophilus hb8. Organism_taxid: 300852. Strain: hb8, dsm 579. Atcc: 27634. Gene: ispd, ttha0171. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (203 residues) CATH domain: 3.90.550.10
2vsh
Synthesis of cdp-activated ribitol for teichoic acid precursors in streptococcus pneumoniae
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Strain: r36a. Atcc: 12214
Chains: A, B (223 residues) CATH domain: 3.90.550.10
2vsi
Synthesis of cdp-activated ribitol for teichoic acid precursors in streptococcus pneumoniae
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Strain: r36a. Atcc: 12214
Chains: A, B (225 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group CDF is 86.00% similar to enzyme reactant CTP
2xwl
Crystal structure of ispd from mycobacterium smegmatis in co with ctp and mg
Source: Mycobacterium smegmatis. Organism_taxid: 1772. Strain: mc2. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: ai.
Chains: A, B (221 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group CTP corresponds to enzyme reactant CTP
2xwm
Crystal structure of ispd from mycobacterium smegmatis in co with cmp
Source: Mycobacterium smegmatis. Organism_taxid: 246196. Strain: mc2. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: ai.
Chains: A, B (220 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group C5P is 72.41% similar to enzyme reactant CTP
2xwn
Crystal structure of ispd from mycobacterium tuberculosis in with ctp and mg
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: ai.
Chains: A, B (221 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group CTP corresponds to enzyme reactant CTP
2yc3
Inhibitors of the herbicidal target ispd
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: prep4.
Chain: A (219 residues) CATH domain: 3.90.550.10
2yc5
Inhibitors of the herbicidal target ispd
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: prep4.
Chain: A (217 residues) CATH domain: 3.90.550.10
2ycm
Inhibitors of herbicidal target ispd
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: prep4.
Chain: A (213 residues) CATH domain: 3.90.550.10
3f1c
Crystal structure of 2-c-methyl-d-erythritol 4-phosphate cytidylyltransferase from listeria monocytogenes
Source: Listeria monocytogenes str. 4b f2365. Organism_taxid: 265669. Gene: ispd2, lmof2365_1100. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (229 residues) CATH domain: 3.90.550.10
3n9w
Crystal structure of 2-c-methyl-d-erythritol 4-phosphate cytidylyltransferase (ispd) in complex with 1,2-propanediol
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12
Chains: A, B (195 residues) CATH domain: 3.90.550.10
3okr
Structure of mtb apo 2-c-methyl-d-erythritol 4-phosphate cytidyltransferase (ispd)
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Gene: ispd, rv3582c, mt3688, mtcy06g11.29c. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D (203 residues) CATH domain: 3.90.550.10
3q7u
Structure of mtb 2-c-methyl-d-erythritol 4-phosphate cytidyltransferase (ispd) complexed with ctp
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Gene: ispd, rv3582c, mt3688, mtcy06g11.29c. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (222 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group CTP corresponds to enzyme reactant CTP
3q80
Structure of mtb 2-c-methyl-d-erythritol 4-phosphate cytidyltransferase (ispd) complexed with cdp-me
Source: Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Gene: ispd, rv3582c, mt3688, mtcy06g11.29c. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (218 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group CDM corresponds to enzyme product 4-(cytidine
4kt7
The crystal structure of 4-diphosphocytidyl-2c-methyl-d- erythritolsynthase from anaerococcus prevotii dsm 20548
Source: Anaerococcus prevotii. Peptostreptococcus prevotii. Organism_taxid: 525919. Strain: dsm 20548. Gene: apre_0175, ispd. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (227 residues) CATH domain: 3.90.550.10
4myb
Crystal structure of francisella tularensis 2-c-methyl-d-ery phosphate cytidylyltransferase (ispd)
Source: Francisella tularensis. Organism_taxid: 263. Gene: ispd, ftl_1525. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (217 residues) CATH domain: 3.90.550.10
4nai
Arabidopsis thaliana ispd apo
Source: Arabidopsis thaliana. Mouse-ear cress, thale-cress. Organism_taxid: 3702. Gene: at2g02500, ispd, mct, mect, mepct, t8k22.20. Expressed in: escherichia coli. Expression_system_taxid: 1007065.
Chain: A (214 residues) CATH domain: 3.90.550.10
4nak
Arabidopsis thaliana ispd in complex with pentabromo-pseudil
Source: Arabidopsis thaliana. Mouse-ear cress, thale-cress. Organism_taxid: 3702. Gene: at2g02500, ispd, mct, mect, mepct, t8k22.20. Expressed in: escherichia coli. Expression_system_taxid: 1007065.
Chain: A (209 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group DTT is 40.00% similar to enzyme reactant 2-C-methyl-D-erythritol
4nal
Arabidopsis thaliana ispd in complex with tribromodichloro-p
Source: Arabidopsis thaliana. Mouse-ear cress, thale-cress. Organism_taxid: 3702. Gene: at2g02500, ispd, mct, mect, mepct, t8k22.20. Expressed in: escherichia coli. Expression_system_taxid: 1007065.
Chain: A (213 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group DTT is 40.00% similar to enzyme reactant 2-C-methyl-D-erythritol
4nan
Arabidopsis thaliana ispd in complex with tetrabromo-pseudil
Source: Arabidopsis thaliana. Mouse-ear cress, thale-cress. Organism_taxid: 3702. Gene: at2g02500, ispd, mct, mect, mepct, t8k22.20. Expressed in: escherichia coli. Expression_system_taxid: 1007065.
Chain: A (208 residues) CATH domain: 3.90.550.10
Bound ligand:   Het Group DTT is 40.00% similar to enzyme reactant 2-C-methyl-D-erythritol