_EC 2.6.1.16 Glutamine--fructose-6-phosphate transaminase (isomerizing). 23 PDB entries  
EC 2.-.-.- Transferases. [17,949 PDB entries]
EC 2.6.-.- Transferring nitrogenous groups. [554 PDB entries]
EC 2.6.1.- Transaminases (aminotransferases). [533 PDB entries]
EC 2.6.1.16 Glutamine--fructose-6-phosphate transaminase (isomerizing). [23 PDB entries]    
1gdo

Pathway: UDP-N-acetylglucosamine Biosynthesis
Reaction: L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.
 


L-glutamine
+
D-fructose 6-phosphate
=
L-glutamate
+
D-glucosamine 6-phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): D-fructose-6-phosphate amidotransferase. GlcN6P synthase. Glucosamine-6-phosphate isomerase (glutamine-forming). Glucosamine-6-phosphate synthase. Hexosephosphate aminotransferase. L-glutamine-D-fructose-6-phosphate amidotransferase.
Comments: Although the overall reaction is that of a transferase, the mechanism involves the formation of ketimine between fructose 6-phosphate and a 6-amino group from a lysine residue at the active site, which is subsequently displaced by ammonia (transamidination). Formerly Ec 5.3.1.19.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 23 PDB entries in enzyme class E.C.2.6.1.16

  PDB code Protein
1gdo
Glutaminase domain of glucosamine 6-phosphate synthase complexed with glutamate
Source: Escherichia coli. Strain: 3000hfr. Atcc: 25257. Expressed in: escherichia coli. Synthetic: yes
Chains: A, B, C, D (238 residues) CATH domain: 3.60.20.10
Bound ligand:   Het Group GLU corresponds to enzyme product L-glutamate
1gms
Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate
Source: Escherichia coli. Strain: 3000hfr. Atcc: 25257. Expressed in: escherichia coli.
Chains: A, C, E, G (238 residues) CATH domain: 3.60.20.10
Bound ligand:   Het Group HGA is 90.00% similar to enzyme reactant L-glutamine
1jxa
Glucosamine 6-phosphate synthase with glucose 6-phosphate
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C (608 residues) CATH domains: 3.60.20.10 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group G6Q corresponds to enzyme reactant D-fructose 6-phosphate
1moq
Isomerase domain of glucosamine 6-phosphate synthase complex glucosamine 6-phosphate
Source: Escherichia coli. Organism_taxid: 562. Strain: hfr 3000. Atcc: 25257. Collection: 25257. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (366 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group GLP corresponds to enzyme product D-glucosamine
1mor
Isomerase domain of glucosamine 6-phosphate synthase complex glucose 6-phosphate
Source: Escherichia coli. Organism_taxid: 562. Strain: hfr 3000. Atcc: 25257. Collection: 25257. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (366 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group G6P corresponds to enzyme reactant D-fructose
1mos
Isomerase domain of glucosamine 6-phosphate synthase complex amino-2-deoxyglucitol 6-phosphate
Source: Escherichia coli. Organism_taxid: 562. Strain: 3000hfr. Atcc: atcc 25257. Collection: atcc 25257. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (367 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group AGP corresponds to enzyme product D-glucosamine
1xff
Glutaminase domain of glucosamine 6-phosphate synthase complexed with glutamate
Source: Escherichia coli. Organism_taxid: 562. Strain: 3000hfr. Atcc: 25257. Gene: glms. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (238 residues) CATH domain: 3.60.20.10
Bound ligand:   Het Group GLU corresponds to enzyme product L-glutamate
1xfg
Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate
Source: Escherichia coli. Organism_taxid: 562. Strain: 3000hfr. Atcc: 25257. Gene: glms. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (238 residues) CATH domain: 3.60.20.10
Bound ligand:   Het Group HGA is 90.00% similar to enzyme reactant L-glutamine
2bpl
E.Coli glucosamine-6p synthase in complex with fructose-6p
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C (608 residues) CATH domains: 3.60.20.10 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group F6R corresponds to enzyme reactant D-fructose
2j6h
E. Coli glucosamine-6-p synthase in complex with glucose-6p and 5-oxo-l-norleucine
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (608 residues) CATH domains: 3.60.20.10 3.40.50.10490 3.40.50.10490
Bound ligands:   Het Group ONL is 81.82% similar to enzyme reactant L-glutamine
  Het Group G6Q corresponds to enzyme reactant D-fructose
2poc
The crystal structure of isomerase domain of glucosamine-6-p synthase from candida albicans
Source: Candida albicans. Organism_taxid: 237561. Strain: sc5314. Gene: gfa1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (339 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligands:   Het Group ACT is 40.00% similar to enzyme reactant L-glutamine
  Het Group BG6 corresponds to enzyme reactant D-fructose
2put
The crystal structure of isomerase domain of glucosamine-6-p synthase from candida albicans
Source: Candida albicans. Organism_taxid: 237561. Strain: sc5314. Gene: gfa1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (339 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligands:   Het Group ACT is 40.00% similar to enzyme reactant L-glutamine
  Het Group F6R corresponds to enzyme reactant D-fructose
2puv
The crystal structure of isomerase domain of glucosamine-6-p synthase from candida albicans
Source: Candida albicans. Organism_taxid: 237561. Strain: sc5314. Gene: gfa1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (338 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group M6R corresponds to enzyme product D-glucosamine
2puw
The crystal structure of isomerase domain of glucosamine-6-p synthase from candida albicans
Source: Candida albicans. Organism_taxid: 237561. Strain: sc5314. Gene: gfa1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (340 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group BG6 corresponds to enzyme reactant D-fructose
2v4m
The isomerase domain of human glutamine-fructose-6- phosphate transaminase 1 (gfpt1) in complex with fructose 6-phosphate
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D (352 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group F6R corresponds to enzyme reactant D-fructose 6-phosphate
2vf4
E. Coli glucosamine-6-p synthase
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: X (359 residues) CATH domains: 3.40.50.10490 3.40.50.10490
2vf5
Glucosamine-6-phosphate synthase in complex with glucosamine-6-phosphate
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: X (366 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group GLP corresponds to enzyme product D-glucosamine
2zj3
Isomerase domain of human glucose:fructose-6-phosphate amidotransferase
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (365 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group G6P corresponds to enzyme reactant D-fructose 6-phosphate
2zj4
Isomerase domain of human glucose:fructose-6-phosphate amidotransferase
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (365 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group AGP corresponds to enzyme product D-glucosamine 6-phosphate
3hba
Crystal structure of a putative phosphosugar isomerase (sden from shewanella denitrificans os217 at 2.00 a resolution
Source: Shewanella denitrificans os217. Organism_taxid: 318161. Gene: sden_2705, yp_563707.1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (311 residues) CATH domains: 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group CIT is 64.29% similar to enzyme product L-glutamate
3ooj
C1a mutant of e. Coli glms in complex with glucose-6p and gl
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H (608 residues) CATH domains: 3.60.20.10 3.40.50.10490 3.40.50.10490
3tbf
C-terminal domain of glucosamine-fructose-6-phosphate aminot from francisella tularensis.
Source: Francisella tularensis subsp. Tularens organism_taxid: 119856. Strain: schu s4. Gene: ftt_0388, glms. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D, E, F, G, H (357 residues) CATH domains: 3.40.50.10490 3.40.50.10490
4amv
E.Coli glucosamine-6p synthase in complex with fructose-6p
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 634468.
Chains: A, B, C (608 residues) CATH domains: 3.60.20.10 3.40.50.10490 3.40.50.10490
Bound ligand:   Het Group F6R corresponds to enzyme reactant D-fructose