_EC 2.5.1.47 Cysteine synthase. 19 PDB entries  
EC 2.-.-.- Transferases. [7,088 PDB entries]
EC 2.5.-.- Transferring alkyl or aryl groups, other than methyl groups. [491 PDB entries]
EC 2.5.1.- Transferring alkyl or aryl groups, other than methyl groups. [491 PDB entries]
EC 2.5.1.47 Cysteine synthase. [19 PDB entries]    
1d6s

Reaction: O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.
 


O-acetyl-L-serine
+ hydrogen sulfide
=
L-cysteine
+
acetate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Acetylserine sulfhydrylase. Cysteine synthetase. O(3)-acetyl-L-serine acetate-lyase (adding hydrogen-sulfide). O-acetyl-L-serine sulfhydrylase. O-acetyl-L-serine sulfohydrolase. O-acetylserine (thiol)-lyase. O-acetylserine (thiol)-lyase A. O-acetylserine sulfhydrylase. Oas sulfhydrylase.
Cofactor(s): Pyridoxal 5'-phosphate.
 

Pyridoxal 5'-phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
Comments: Some alkyl thiols, cyanide, pyrazole and some other heterocyclic compounds can act as acceptors. Not identical with Ec 2.5.1.51, Ec 2.5.1.52 and Ec 2.5.1.53. Formerly Ec 4.2.99.8.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 19 PDB entries in enzyme class E.C.2.5.1.47

  PDB code Protein
1d6s
Crystal structure of the k41a mutant of o-acetylserine sulfh complexed in external aldimine linkage with methionine
Source: Salmonella typhimurium. Organism_taxid: 602. Strain: dw378. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (322 residues) CATH domains: 3.40.50.1100 3.40.50.1100
Bound ligands:   Het Group MET is 60.00% similar to enzyme product L-cysteine
  Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
1oas
O-acetylserine sulfhydrylase from salmonella typhimurium
Source: Salmonella typhimurium. Organism_taxid: 602. Expressed in: salmonella typhimurium. Expression_system_taxid: 602
Chains: A, B (315 residues) CATH domains: 3.40.50.1100 3.40.50.1100
Bound ligand:   Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
1wkv
Crystal structure of o-phosphoserine sulfhydrylase
Source: Aeropyrum pernix. Organism_taxid: 272557. Strain: k1. Gene: ape1586. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (382 residues) CATH domains: Unassigned 3.40.50.1100 3.40.50.1100
Bound ligands:   Het Group ACT corresponds to enzyme product acetate
  Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
1z7y
Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Gene: oasa1, oas1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (320 residues) CATH domains: 3.40.50.1100 3.40.50.1100
Bound ligand:   Het Group AA5 is 60.00% similar to enzyme cofactor pyridoxal
2egu
Crystal structure of o-acetylserine sulfhydrase from geobaci kaustophilus hta426
Source: Geobacillus kaustophilus. Organism_taxid: 235909. Strain: hta426. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (271 residues) CATH domains: 3.40.50.1100 3.40.50.1100
2jc3
Structure of o-acetylserine sulfhydrylase b from salmonella typhimurium
Source: Salmonella typhimurium. Organism_taxid: 602. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: B, C, D, E, F, G, H ( residues)
Bound ligand:   Het Group PLP is 93.00% similar to enzyme cofactor pyridoxal 5'-phosphate
2q3b
1.8 a resolution crystal structure of o-acetylserine sulfhyd (oass) holoenzyme from mycobacterium tuberculosis
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Strain: rv2334. Gene: cysk. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (300 residues) CATH domains: 3.40.50.1100 3.40.50.1100
Bound ligand:   Het Group MPD is 41.18% similar to enzyme cofactor pyridoxal
2q3c
2.1 a resolution crystal structure of o-acetylserine sulfhyd (oass) holoenzyme from mycobacterium tuberculosis in comple inhibitory peptide dfsi
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Strain: rv2334. Gene: cysk. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 1773
Chain: A (300 residues) CATH domains: 3.40.50.1100 3.40.50.1100
Bound ligands:   Het Group SER is 60.00% similar to enzyme reactant O-acetyl-L-serine
  Het Group ILE is 60.00% similar to enzyme product L-cysteine
  Het Group ASP is 50.00% similar to enzyme product acetate
  Het Group PHE is 42.00% similar to enzyme cofactor pyridoxal 5'-phosphate
3fca
Genetic incorporation of a metal-ion chelating amino acid into proteins as biophysical probe
Source: Thermus thermophilus. Organism_taxid: 274. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (291 residues) CATH domains: 3.40.50.1100 3.40.50.1100
3iqi
Structure of o-acetylserine sulfhydrylase in complex with peptide mneni
Source: Haemophilus influenzae. Organism_taxid: 727. Gene: cysk, hi1103. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: X (310 residues) CATH domains: 3.40.50.1100 3.40.50.1100
Bound ligands:   Het Group ILE is 60.00% similar to enzyme product L-cysteine
  Het Group GLU is 44.00% similar to enzyme product acetate
3vsc
Crystal structure of the k127a mutant of o-phosphoserine sul complexed with external schiff base of pyridoxal 5'-phospha phospho-l-serine
Source: Aeropyrum pernix. Organism_taxid: 272557. Strain: k1. Gene: ape1586, ape_1586, cyso. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (382 residues) CATH domains: Unassigned 3.40.50.1100 3.40.50.1100
3vsd
Crystal structure of the k127a mutant of o-phosphoserine sul complexed with external schiff base of pyridoxal 5'-phospha acetyl-l-serine
Source: Aeropyrum pernix. Organism_taxid: 272557. Strain: k1. Gene: ape1586, ape_1586, cyso. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (382 residues) CATH domains: Unassigned 3.40.50.1100 3.40.50.1100
4air
Leishmania major cysteine synthase
Source: Leishmania major. Organism_taxid: 5664. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (304 residues) CATH domains: 3.40.50.1100 3.40.50.1100
Bound ligand:   Het Group FGA is 60.00% similar to enzyme product L-cysteine
4i1x
Crystal structure of cysteine synthase from helicobacter pyl
Source: Helicobacter pylori. Organism_taxid: 85962. Strain: atcc 700392 / 26695. Gene: cysk, cysm, cysteine synthase, hp_0107. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (306 residues) CATH domains: 3.40.50.1100 3.40.50.1100
4i1y
The structure of cysteine synthase from mycobacterium ulcera
Source: Mycobacterium ulcerans. Organism_taxid: 362242. Strain: agy99. Gene: cysk1, mul_1465. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: B, C, D ( residues)
4li3
Crystal structure of o-acetylserine sulfhydrylase from haemo influenzae in complex with high affinity inhibitory peptide serine acetyl transferase of salmonella typhimurium
Source: Haemophilus influenzae. Organism_taxid: 727. Strain: atcc 51907. Gene: cysk, hi_1103. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Salmonella typhimurium. Organism_taxid: 990282.
Chain: X (311 residues) CATH domains: Unassigned Unassigned
Bound ligands:   Het Group THR is 54.55% similar to enzyme reactant O-acetyl-L-serine
  Het Group ILE is 60.00% similar to enzyme product L-cysteine
  Het Group GLU is 44.44% similar to enzyme product acetate
  Het Group PHE is 42.11% similar to enzyme cofactor pyridoxal
4lma
Crystal structure analysis of o-acetylserine sulfhydrylasE C microcystis aeruginosa 7806
Source: Microcystis aeruginosa. Organism_taxid: 267872. Strain: pcc 7806. Gene: cysk, ipf_2058. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (318 residues) CATH domains: Unassigned Unassigned
Bound ligand:   Het Group PLP is 93.75% similar to enzyme cofactor pyridoxal
4mh9
Crystal structure of o-acetylserine sulfhydrylase from haemo influenzae in complex with pre-reactive o-acetyl serine and inhibitor
Source: Haemophilus influenzae. Organism_taxid: 71421. Strain: atcc51907. Gene: cysk, hi_1103. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Chain: X (309 residues) CATH domains: Unassigned Unassigned
Bound ligands:   Het Group OAS corresponds to enzyme reactant O-acetyl-L-serine
  Het Group GLU is 44.44% similar to enzyme product acetate
  Het Group PLP corresponds to enzyme cofactor pyridoxal
4r2v
Crystal structure of cysteine synthase from helicobacter pyl
Source: Helicobacter pylori 26695. Organism_taxid: 85962. Strain: atcc 700392 / 26695. Gene: c694_00530, cysk, cysm, cysteine synthase, hp_0107. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (306 residues) CATH domains: Unassigned 3.40.50.1100